Evaluation of Drought Tolerance and Trehalose Response in Auricularia heimuer DOI Creative Commons
Jian Sun,

Fangjie Yao,

Lixin Lu

et al.

Horticulturae, Journal Year: 2024, Volume and Issue: 10(12), P. 1312 - 1312

Published: Dec. 10, 2024

Auricularia heimuer is drought tolerant, but the mechanism underlying its physiological response to has not been systematically studied. We selected 13 strains of A. and simulated stress using a complete yeast medium (CYM) containing 20% polyethylene glycol (PEG), while used for control treatments did contain PEG. Strains were cultured on shaker incubator at 25 °C 120 rpm 15 days under dark conditions. The contents soluble sugars (SS) proteins (SP), activities superoxide dismutase (SOD) catalase (CAT), content malondialdehyde (MDA), biomass measured. Between PEG treatment control, as well among different strains, there significant differences in all indices. tested classified into following four categories according their membership function values: first category consisted highly drought-tolerant strain A; second A127 C; third moderately A124, A14, A386, A462, A184, A496, A125, B; fourth drought-sensitive A356 A508. Transcriptome analysis was performed C before after treatment, 1762 differentially expressed genes (DEGs) obtained, including 798 up- 964 down-regulated genes. Through KEGG enrichment analysis, it found time that synthesis pathway trehalose phosphate synthase–trehalose phosphatase (TPS-TPP), which involved stress. In addition, two key enzyme synthesis, namely trehalose-6-phosphate synthase (AhTPS) (AhTPP), significantly up-regulated increased 11 treatment. This study discovered, time, edible fungi stress, thus providing reference genetic improvement selection laying theoretical foundation resistance breeding other fungi.

Language: Английский

Adaptation of the psychrophilic Mucor psychrophilus (Mucorales, Mucoromycota) to lower temperatures and under conditions of heat and osmotic shocks DOI
О. А. Данилова, Elena A. Ianutsevich, G. А. Kochkina

et al.

Fungal Biology, Journal Year: 2025, Volume and Issue: 129(2), P. 101532 - 101532

Published: Jan. 10, 2025

Language: Английский

Citations

0
Application of UHPLC-ESI-MS/MS-Based Metabonomic Techniques to Analyze the Cordyceps cicadae Metabolic Profile Changes to the CO(NH2)2 Response Mechanism in the Process of Ergosterol Synthesis DOI Creative Commons
Miaomiao Wang, Zhicai Zhang,

Fenghui SHI

et al.

Fermentation, Journal Year: 2025, Volume and Issue: 11(2), P. 90 - 90

Published: Feb. 11, 2025

Nitrogen source is a necessary nutrient factor in the process of mycelial growth and metabolite synthesis. To improve yield ergosterol, previous study, we used transcriptome technology to explore difference gene expression Cordyceps cicadae by CO(NH2)2 (urea) synthesizing ergosterol. In present was examine its effects on cell membrane permeability metabolic flow C. fermentation ergosterol Metabonomic results showed that supplementation caused significant changes five aspects: (1) notably increased biomass extracellular decreased intracellular concentration; (2) boosted level inositol 1,4-bisphosphate, which implied acceleration decomposition, weakening integrity, increase change regionalization; (3) changed fluxes speed, including increasing levels amino acids, vitamins, hormones, nucleotides, exhibited an elevated growth, promoting synthesis flavonoids, alkaloids, terpenes, facilitating decreasing degradation ergosterol; (4) anaphase, inhibition 6-phosphogluconate dehydrogenase α-ketoglutarate activities, NADPH, NADH, ATP synthesis, finally inhibited Collectively, metabonomics valuable strategy study regulatory medium composition incubation conditions cicadae.

Language: Английский

Citations

0
Structural Optimization and Discovery of High Effectiveisopropanolamine-Based Tps1 Inhibitors as Promising Broad-Spectrum Fungicide Candidates DOI
Zhiyang Jiang, Na Wang,

Jinxiu Chen

et al.

Published: Jan. 1, 2025

Language: Английский

Citations

0
Structural Optimization and Discovery of High Effective Isopropanolamine-Based TPS1 Inhibitors as Promising Broad-Spectrum Fungicide Candidates DOI
Zhiyang Jiang, Na Wang,

Jinxiu Chen

et al.

European Journal of Medicinal Chemistry, Journal Year: 2025, Volume and Issue: 290, P. 117553 - 117553

Published: March 24, 2025

Language: Английский

Citations

0
Maize–Fusarium associations and their mycotoxins: Insights from South Africa DOI Creative Commons
Cobus M. Visagie, Hannalien Meyer, Neriman Yılmaz

et al.

Fungal Biology, Journal Year: 2024, Volume and Issue: 128(8), P. 2408 - 2421

Published: March 28, 2024

For maize, a staple food in South Africa, there is lack of comprehensive knowledge on the mycotoxin-producing fungal diversity. In this study, community profile was established using culture-dependent methods for 56 maize seed samples that were also analysed 13 mycotoxins. The isolates identified by morphology and DNA sequencing. A total 723 from 21 genera 99 species obtained characterised. Fusarium most common genus (isolated 52 samples), followed Cladosporium (n = 45), Aspergillus 41), Talaromyces 40), Penicillium 38). communities dominated fujikuroi complex, which includes such as verticillioides temperatum, while awaxy mirum are reported here first time Africa. As deoxynivalenol (DON) producing species, only boothii graminearum isolated to lesser extent. DON 37), fumonisins (FUM) 32), zearalenone (ZEA) 6) detected. presence particular did not guarantee corresponding mycotoxins, inverse true. occurrence and/or FUM African remains health concern, so continuous monitoring both their mycotoxins important.

Language: Английский

Citations

1
Trehalose Protects against Superoxide Dismutase 1 Proteinopathy in an Amyotrophic Lateral Sclerosis Model DOI Creative Commons
Rayne Stfhany Silva Magalhães, José Raphael Monteiro Neto,

Gabriela D. Ribeiro

et al.

Antioxidants, Journal Year: 2024, Volume and Issue: 13(7), P. 807 - 807

Published: July 3, 2024

This work aimed to study the effect of trehalose in protecting cells against Sod1 proteinopathy associated with amyotrophic lateral sclerosis (ALS). Humanized yeast which native was replaced by wild-type human or an ALS mutant (WT-A4V heterodimer) were used as experimental model. Cells treated 10% (p/v) before after appearance hSod1 induced oxidative stress. In both conditions, reduced number inclusions, increased activity, and decreased levels intracellular oxidation, demonstrating that avoids misfolding loss function response The survival rates stressed presence 60% higher than their absence. Treatment inclusions expressing WT doubled longevity; 5 days, non-treated did not survive, but 15% sugar still alive. Altogether, our results emphasize potential a novel therapy, might be applied preventively patients family history disease diagnosis who discover following first symptoms.

Language: Английский

Citations

1
MaPom1, a Dual-Specificity Tyrosine Phosphorylation-Regulated Kinase, Positively Regulates Thermal and UV-B Tolerance in Metarhizium acridum DOI Open Access
Yu Zhang, Lei Song, Yuxian Xia

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(22), P. 11860 - 11860

Published: Nov. 5, 2024

Fungi play irreplaceable roles in the functioning of natural ecosystems, but global warming poses a significant threat to them. However, mechanisms underlying fungal tolerance thermal and UV-B stresses remain largely unknown. Dual-specificity tyrosine phosphorylation-regulated kinase (DYRK) Pom1 is crucial for growth, conidiation, virulence. its role stress within kingdom fungi has not been explored. In this study, we analyzed function

Language: Английский

Citations

0
Effect of modified trehalose on regulation of gluten protein structure and steamed bread quality DOI
Tongchao Su,

Youtong Zhang,

Wenkai Du

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: unknown, P. 137969 - 137969

Published: Nov. 1, 2024

Language: Английский

Citations

0
Evaluation of Drought Tolerance and Trehalose Response in Auricularia heimuer DOI Creative Commons
Jian Sun,

Fangjie Yao,

Lixin Lu

et al.

Horticulturae, Journal Year: 2024, Volume and Issue: 10(12), P. 1312 - 1312

Published: Dec. 10, 2024

Auricularia heimuer is drought tolerant, but the mechanism underlying its physiological response to has not been systematically studied. We selected 13 strains of A. and simulated stress using a complete yeast medium (CYM) containing 20% polyethylene glycol (PEG), while used for control treatments did contain PEG. Strains were cultured on shaker incubator at 25 °C 120 rpm 15 days under dark conditions. The contents soluble sugars (SS) proteins (SP), activities superoxide dismutase (SOD) catalase (CAT), content malondialdehyde (MDA), biomass measured. Between PEG treatment control, as well among different strains, there significant differences in all indices. tested classified into following four categories according their membership function values: first category consisted highly drought-tolerant strain A; second A127 C; third moderately A124, A14, A386, A462, A184, A496, A125, B; fourth drought-sensitive A356 A508. Transcriptome analysis was performed C before after treatment, 1762 differentially expressed genes (DEGs) obtained, including 798 up- 964 down-regulated genes. Through KEGG enrichment analysis, it found time that synthesis pathway trehalose phosphate synthase–trehalose phosphatase (TPS-TPP), which involved stress. In addition, two key enzyme synthesis, namely trehalose-6-phosphate synthase (AhTPS) (AhTPP), significantly up-regulated increased 11 treatment. This study discovered, time, edible fungi stress, thus providing reference genetic improvement selection laying theoretical foundation resistance breeding other fungi.

Language: Английский

Citations

0