PHI-base – the multi-species pathogen–host interaction database in 2025

Nucleic Acids Research, Journal Year: 2024, Volume and Issue: 53(D1), P. D826 - D838

Published: Nov. 26, 2024

The Pathogen-Host Interactions Database (PHI-base) has, since 2005, provided manually curated genes from fungal, bacterial and protist pathogens that have been experimentally verified to important pathogenicity, virulence and/or effector functions during different types of interactions involving human, animal, plant, invertebrate fungal hosts. PHI-base provides phenotypic annotation genotypic information for both native model host interactions, including gene alterations do not alter the phenotype interaction. In this article, we describe major updates PHI-base. latest version PHI-base, 4.17, contains a 19% increase in 23% relative 4.12 (released September 2022). We also unification data 4 with new curation workflow (PHI-Canto), which forms first complete release 5.0. Additionally, adding support Frictionless Data framework datasets, ways sharing interaction Ensembl database, an analysis conserved orthologous increasing variety research studies make use 4.17 is freely available at www.phi-base.org 5.0 phi5.phi-base.org.

Language: Английский

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A conserved fungal Knr4/Smi1 protein is crucial for maintaining cell wall stress tolerance and host plant pathogenesis

PLoS Pathogens, Journal Year: 2025, Volume and Issue: 21(1), P. e1012769 - e1012769

Published: Jan. 9, 2025

Filamentous plant pathogenic fungi pose significant threats to global food security, particularly through diseases like Fusarium Head Blight (FHB) and Septoria Tritici Blotch (STB) which affects cereals. With mounting challenges in fungal control increasing restrictions on fungicide use due environmental concerns, there is an urgent need for innovative strategies. Here, we present a comprehensive analysis of the stage-specific infection process graminearum wheat spikes by generating dual weighted gene co-expression network (WGCN). Notably, contained mycotoxin-enriched module (F12) that exhibited correlation with detoxification gene-enriched (W12). This expression was validated quantitative PCR. By examining genes highly expressed during early symptomless correlated enriched oxidative stress genes, identified encoding FgKnr4, protein containing Knr4/Smi1 disordered domain. Through analysis, confirmed pivotal role FgKnr4 various biological processes, including tolerance, cell cycle morphogenesis, growth, pathogenicity. Further studies observed phenotypes are partially involvement regulating wall integrity pathway modulating phosphorylation MAP-kinase MGV1. Orthologues widespread across kingdom but absent other Eukaryotes, suggesting has potential as promising intervention target. Encouragingly, restricted growth reduced virulence ΔFgknr4 were replicated upon deletion orthologous pathogen Zymoseptoria tritici . Overall, this study demonstrates utility integrated network-level analytical approach pinpoint high interest pathogenesis disease control.

Language: Английский

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Gut bacterial and fungal communities of François’ langur (Trachypithecus francoisi) changed coordinate to different seasons

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: March 5, 2025

François' langur (Trachypithecus francoisi), an endangered primate endemic to limestone forests in Vietnam and China, relies on gut microbiota maintain gastrointestinal stability adapt dietary shifts. While microbial communities are dynamic sensitive seasonal resource variations, their specific responses langurs remain poorly characterized. This study investigates variations the composition diversity of bacterial fungal this species enhance understanding its ecological adaptations. Fresh fecal samples from 22 Mayanghe National Nature Reserve, were collected across four seasons. Bacterial analyzed using high-throughput sequencing assess taxonomic α-diversity. Statistical comparisons conducted evaluate differences at phylum genus levels. Significant shifts occurred both communities. α-diversity peaked warmer seasons, whereas was higher colder months. At level, Akkermansia (1.3% relative abundance summer), a mucin-degrading bacterium linked health, dominated In contrast, Cercophora, associated with plant biomass degradation, enriched during Seasonal factors strongly influenced structure, distinct community assemblages observed all The inverse patterns suggest complementary roles nutrient extraction under constraints. Akkermansia's summer prevalence may reflect enhanced mucin utilization fruit-rich periods, while Cercophora's cold-season dominance likely aids cellulose breakdown leaf-heavy diets. These dynamics highlight microbiota's role optimizing energy harvest seasonally variable By elucidating plasticity, provides critical insights for developing conservation strategies tailored nutritional ecology langurs.

Language: Английский

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Wheat resistance to Fusarium head blight and breeding strategies

Crop Health, Journal Year: 2025, Volume and Issue: 3(1)

Published: April 8, 2025

Language: Английский

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TheFusarium graminearumeffector protease FgTPP1 suppresses immune responses and facilitates Fusarium Head Blight Disease

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 2, 2024

ABSTRACT Most plant pathogens secrete effector proteins to circumvent host immune responses, thereby promoting pathogen virulence. One such is the fungus Fusarium graminearum , which causes Head Blight (FHB) disease on wheat and barley. Transcriptomic analyses revealed that F. expresses many candidate during early phases of infection process, some are annotated as proteases. However, contributions these proteases virulence remains poorly defined. Here, we characterize a endopeptidase, FgTPP1 (FGSG_11164), highly upregulated spikelet secreted from fungal cells. To elucidate potential role in virulence, generated deletion mutants ( ΔFgtpp1 ) performed FHB assays. While number completely bleached spikes infected by F . wild-type reached 50% total spikes, fully was 25%, suggesting contributes Transient expression green fluorescent protein (GFP)-tagged localizes, part, chloroplasts attenuates chitin-mediated activation mitogen-activated kinase (MAPK) signaling, reactive oxygen species production, cell death induced an autoactive resistance when expressed planta Notably, conserved across Ascomycota phylum, making it core among ascomycete pathogens. These properties make ideal for decoy substrate engineering, with goal engineering FHB, likely other crop diseases caused fungi.

Language: Английский

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Characterization of Fusarium falciforme inciting wilt in peace lily (Spathiphyllum wallisii)

Scientia Horticulturae, Journal Year: 2024, Volume and Issue: 339, P. 113834 - 113834

Published: Nov. 29, 2024

Language: Английский

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