Calcium-Induced Regulation of Sanghuangporus baumii Growth and the Biosynthesis of Its Triterpenoids DOI Creative Commons
Zengcai Liu,

Ying Yu,

Shiyuan Wang

et al.

Journal of Fungi, Journal Year: 2025, Volume and Issue: 11(3), P. 238 - 238

Published: March 20, 2025

Sanghuangporus baumii, a fungus used in traditional Chinese medicine, produces important pharmacological compounds such as triterpenoids, but at levels significantly lower than those required for medical use. This study investigated the effects of various concentrations Ca2+ on S. baumii mycelial growth and heterologous biosynthesis triterpenoids. Under induction by 10 mM Ca2+, rate (0.39 cm/d) biomass (4.48 g/L) mycelia were 1.03% 10.05% higher 0 Ca2+-treatment group, respectively. In contrast, 200 inhibited mycelia. Notably, total triterpenoid content reached its peak (17.71 mg/g) with significant increase (3869.97 µg/g) Subsequently, differential metabolic pathways related genes between groups examined using transcriptomic analysis. The results indicated that was primarily due to elevated soluble sugar content, whereas inhibition associated toxic H2O2. observed differences mainly attributed activation terpenoid backbone pathway AACT gene. Finally, gene cloned transformed into yeast cells, thus creating strain Sc-AA1. Upon treatment optimal concentration, squalene Sc-AA1 0.78 mg/g, 2.89-fold control group. These findings are triterpenoids from baumii. Our demonstrates feasibility producing Saccharomyces cerevisiae provides foundation future optimization toward achieving industrially relevant yields.

Language: Английский

Calcium-Induced Regulation of Sanghuangporus baumii Growth and the Biosynthesis of Its Triterpenoids DOI Creative Commons
Zengcai Liu,

Ying Yu,

Shiyuan Wang

et al.

Journal of Fungi, Journal Year: 2025, Volume and Issue: 11(3), P. 238 - 238

Published: March 20, 2025

Sanghuangporus baumii, a fungus used in traditional Chinese medicine, produces important pharmacological compounds such as triterpenoids, but at levels significantly lower than those required for medical use. This study investigated the effects of various concentrations Ca2+ on S. baumii mycelial growth and heterologous biosynthesis triterpenoids. Under induction by 10 mM Ca2+, rate (0.39 cm/d) biomass (4.48 g/L) mycelia were 1.03% 10.05% higher 0 Ca2+-treatment group, respectively. In contrast, 200 inhibited mycelia. Notably, total triterpenoid content reached its peak (17.71 mg/g) with significant increase (3869.97 µg/g) Subsequently, differential metabolic pathways related genes between groups examined using transcriptomic analysis. The results indicated that was primarily due to elevated soluble sugar content, whereas inhibition associated toxic H2O2. observed differences mainly attributed activation terpenoid backbone pathway AACT gene. Finally, gene cloned transformed into yeast cells, thus creating strain Sc-AA1. Upon treatment optimal concentration, squalene Sc-AA1 0.78 mg/g, 2.89-fold control group. These findings are triterpenoids from baumii. Our demonstrates feasibility producing Saccharomyces cerevisiae provides foundation future optimization toward achieving industrially relevant yields.

Language: Английский

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