Journal of Applied Microbiology,
Journal Year:
2024,
Volume and Issue:
135(7)
Published: July 1, 2024
Abstract
Aim
In
this
study,
we
investigated
culturable
yeast
community,
present
in
grape
must
sampled
from
vineyards
with
apiaries
on
the
borders,
and
honey
bees
collected
these
apiaries.
Methods
results
To
aim,
yeasts
isolated
spontaneously
fermented
grapes
randomly
two
(P1
P2)
borders
(A1
A2)
were
compared
to
those
a
vineyard
without
apiary
(P4).
At
same
time,
community
was
analyzed
each
placed
vineyards,
comparison
an
(A3)
located
far
vineyards.
The
analysis
performed
for
consecutive
years
(2021
2022).
identified
by
restriction
of
amplified
ITS
region,
followed
sequencing
fragment.
Our
research
showed
that
presence
seems
increase
counts
must,
particular
Saccharomyces
cerevisiae;
furthermore,
permanence
allowed
recovering
also
bees.
Conclusions
findings
seem
corroborate
role
as
vectors
reservoirs
oenologically
relevant
yeasts,
such
source
non-conventional
potential
biotechnological
applications.
Makara Journal of Science,
Journal Year:
2024,
Volume and Issue:
28(1)
Published: March 25, 2024
Mead,
an
ancient
alcoholic
beverage,
relies
on
the
indigenous
microbial
community
in
honey
for
alcohol
fermentation.
Nevertheless,
data
use
of
bee
flora
mead
production
Thailand
are
scarce.
This
investigation
involved
isolation
alcohol-producing
yeast
strains
from
Thai
stingless
species
Heterotrigona
itama.
Subsequently,
these
isolated
were
employed
fermentation
with
sourced
same
species.
Findings
revealed
that
strain
denoted
as
P03b,
which
was
identified
molecularly
Saccharomyces
cerevisiae,
exhibited
highest
13.53%
±
0.76%.
Furthermore,
P03b
demonstrated
notable
stress
tolerance,
including
resistance
to
elevated
osmotic
pressure
(30%
w/v
glucose),
extreme
pH
conditions
(pH
2),
and
high
ethanol
content
(20%
w/v).
In
fermentation,
observed
concentration
reached
10.20%
0.26%.
The
resultant
had
a
3.54%
0.04%
total
reducing
sugar
0.04
0.00
mg/mL.
study
provides
new
knowledge
using
combs
beverage
production.
Sociobiology,
Journal Year:
2024,
Volume and Issue:
71(2), P. e10359 - e10359
Published: May 31, 2024
The
interaction
between
stingless
bees
and
fungi
has
garnered
recent
interest
due
to
potential
mutual
benefits.
In
Brazil,
Melipona
scutellaris
stands
out
ecologically
economically.
We
investigated
its
microbiota,
focusing
on
yeast
species
in
pot-honey,
pot-pollen,
nest
surfaces.
Samples
from
the
Atlantic
Forest
(Pernambuco
state,
Brazil)
were
analyzed
through
morphological
physiological
methods
D1/D2
LSU
rDNA
sequence
analysis.
identified
20
species,
comprising
15
ascomycetes
five
basidiomycetes,
representing
11
genera,
respectively.
All
except
Blastobotrys
meliponae,
reported
for
first
time
association
with
M.
scutellaris.
Honey
exhibited
highest
richness.
this
study,
Meyerozyma
caribbica,
Wickerhamomyces
anomalus,
Moniliella
carnis
exclusively
isolated
honey.
understanding
of
ecological
yeasts,
as
revealed
our
research,
can
significantly
aid
insect
conservation
programs.
present
report
yeasts
associated
substrates
living
Brazilian
Forest.
Journal of Applied Microbiology,
Journal Year:
2024,
Volume and Issue:
135(7)
Published: July 1, 2024
Abstract
Aim
In
this
study,
we
investigated
culturable
yeast
community,
present
in
grape
must
sampled
from
vineyards
with
apiaries
on
the
borders,
and
honey
bees
collected
these
apiaries.
Methods
results
To
aim,
yeasts
isolated
spontaneously
fermented
grapes
randomly
two
(P1
P2)
borders
(A1
A2)
were
compared
to
those
a
vineyard
without
apiary
(P4).
At
same
time,
community
was
analyzed
each
placed
vineyards,
comparison
an
(A3)
located
far
vineyards.
The
analysis
performed
for
consecutive
years
(2021
2022).
identified
by
restriction
of
amplified
ITS
region,
followed
sequencing
fragment.
Our
research
showed
that
presence
seems
increase
counts
must,
particular
Saccharomyces
cerevisiae;
furthermore,
permanence
allowed
recovering
also
bees.
Conclusions
findings
seem
corroborate
role
as
vectors
reservoirs
oenologically
relevant
yeasts,
such
source
non-conventional
potential
biotechnological
applications.
