OTORHINOLARYNGOLOGY,
Journal Year:
2023,
Volume and Issue:
unknown, P. 91 - 100
Published: Jan. 1, 2023
The
structure
of
the
hearing
organ
combines
simplicity
and
depth,
remaining
in
many
issues
subject
active
scientific
research.
ontogenesis
this
exquisite
mechanism
is
regulated
by
complex
still
incompletely
understood
mechanisms.
technological
tools
modern
biomedical
science
allow
not
only
to
study,
but
also
work
at
cellular
molecular
level.
In
article,
we
review
data
on
realism
restoring
population
human
auditory
receptors
promising
ways
solve
problem.
addition,
offer
an
answer
a
fundamental
question
–
why,
with
small
number
relatively
simply
arranged
receptor
cells
inner
ear,
their
self-restored.
Key
words:
spiral
organ,
stem
cells,
progenitors,
impairments,
regeneration,
bioprosthetics,
bioengineering.
Smart Medicine,
Journal Year:
2024,
Volume and Issue:
3(1)
Published: Jan. 16, 2024
More
than
6%
of
the
world's
population
is
suffering
from
hearing
loss
and
balance
disorders.
The
inner
ear
organ
that
senses
sound
balance.
Although
disorders
are
common,
there
limited
ways
to
intervene
restore
its
sensory
functions.
development
establishment
biologically
therapeutic
interventions
for
auditory
require
clarification
basics
signaling
pathways
control
endogenous
or
exogenous
cell-based
methods.
In
vitro
models
ear,
such
as
organoid
systems,
can
help
identify
new
protective
regenerative
drugs,
develop
gene
therapies,
be
considered
potential
tools
future
clinical
applications.
Advances
in
stem
cell
technology
culture
offer
unique
opportunities
modeling
diseases
developing
personalized
therapies
loss.
Here,
we
review
discuss
mechanisms
applications
organoids.
Development,
Journal Year:
2023,
Volume and Issue:
150(19)
Published: Aug. 10, 2023
ABSTRACT
Our
molecular
understanding
of
the
early
stages
human
inner
ear
development
has
been
limited
by
difficulty
in
accessing
fetal
samples
at
gestational
stages.
As
an
alternative,
previous
studies
have
shown
that
morphogenesis
can
be
partially
recapitulated
using
induced
pluripotent
stem
cells
directed
to
differentiate
into
organoids
(IEOs).
Once
validated
and
benchmarked,
these
systems
could
represent
unique
tools
complement
refine
our
otic
differentiation
model
developmental
defects.
Here,
we
provide
first
direct
comparisons
embryonic
otocyst
sensory
organs
with
IEOs.
We
use
multiplexed
immunostaining
single-cell
RNA-sequencing
characterize
IEOs
three
key
steps,
providing
a
new
signature
vitro-derived
placode,
epithelium,
neuroblasts
epithelia.
In
parallel,
evaluate
expression
localization
crucial
markers
equivalent
embryos.
Together,
data
indicate
current
state-of-the-art
protocol
enables
specification
bona
fide
tissue,
supporting
further
application
inform
biology
disease.
Journal of the Association for Research in Otolaryngology,
Journal Year:
2024,
Volume and Issue:
25(1), P. 5 - 11
Published: Feb. 9, 2024
Abstract
Inner
ear
organoids
derived
from
differentiation
of
human
pluripotent
stem
cells
have
recently
gained
momentum
as
tools
to
study
inner
development
and
developmental
defects.
An
additional
exciting
aspect
about
this
technology
is
represented
by
its
translational
potential,
specifically,
the
use
validate
therapeutics
for
hearing
balance
restoration
on
human/patient-specific
cells.
This
latter
will
be
briefly
discussed
here
including
opportunities
current
limitations.
Advanced Science,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 24, 2025
Abstract
The
auditory
circuit
primarily
consists
of
peripheral
organs
and
neural
pathways.
Hearing
loss
(HL),
as
reported
by
the
World
Health
Organization,
affects
more
than
one
in
eight
people
worldwide,
often
leading
to
severe
dysfunction
that
detrimentally
impacts
patients'
quality
life.
Therefore,
reconstruction
has
emerged
a
persistent
research
hotspot
challenge
within
biomedical
field.
Traditional
methods
for
reconstruction,
such
drug
therapy,
hearing
aids,
cochlear
implants
(CIs),
so
on.
To
certain
extent,
it
can
help
patients
with
HL
improve
their
status.
However,
they
possess
inherent
advantages
limitations.
Recent
advancements
biomaterials,
gene
editing,
stem
cells,
organoids,
other
technologies
have
ushered
new
prospects
treatment
impairment.
This
review
focuses
on
potential
innovative
editing
recovery.
It
reviews
current
status
inner
ear
hair
cell
(HC)
regeneration,
spiral
ganglion
neuron
(SGN)
organoid
construction
loop.
Furthermore,
discusses
challenges
associated
these
approaches
explores
future
directions,
aiming
furnish
comprehensive
reference
both
clinical
applications
domain
restoration.
Development,
Journal Year:
2023,
Volume and Issue:
150(19)
Published: Oct. 1, 2023
ABSTRACT
Inner
ear
development
requires
the
coordination
of
cell
types
from
distinct
epithelial,
mesenchymal
and
neuronal
lineages.
Although
we
have
learned
much
animal
models,
many
details
about
human
inner
remain
elusive.
We
recently
developed
an
in
vitro
model
organogenesis
using
pluripotent
stem
cells
a
3D
culture,
fostering
growth
sensorineural
circuit,
including
hair
neurons.
Despite
previously
characterizing
some
types,
undefined.
This
study
aimed
to
chart
timeline
organoid
understand
mechanisms
at
play.
Using
single-cell
RNA
sequencing
ten
stages
during
first
36
days
differentiation,
tracked
evolution
pluripotency
various
after
exposure
specific
signaling
modulators.
Our
findings
showcase
gene
expression
that
influences
identifying
plethora
ectodermal
types.
also
discern
aspects
consistent
with
vivo
development,
while
highlighting
potential
discrepancies.
establishes
Ear
Organoid
Developmental
Atlas
(IODA),
offering
deeper
insights
into
biology
improving
tissue
differentiation.
Journal of Biomedical Materials Research Part B Applied Biomaterials,
Journal Year:
2024,
Volume and Issue:
112(7)
Published: June 24, 2024
Sensorineural
hearing
loss
(SNHL)
is
mainly
caused
by
injury
or
of
hair
cells
(HCs)
and
associated
spiral
ganglion
neurons
(SGNs)
in
the
inner
ear.
At
present,
there
still
no
effective
treatment
for
SNHL
clinic.
Recently,
advances
organoid
bring
a
promising
prospect
research
SNHL.
Meanwhile,
three-dimensional
(3D)
printing
provides
tremendous
opportunity
to
construct
versatile
organoids
tissue
engineering
regenerative
medicine.
In
this
study,
gelatin
(Gel),
sodium
alginate
(SA),
polyvinyl
alcohol
(PVA)
were
used
fabricate
biomimetic
scaffold
through
3D
printing.
The
organ
Corti
derived
from
neonatal
mice
ear
was
seeded
on
PVA/Gel/SA
organoid.
Then,
study
potential
protective
effects
berberine
sulfate
neomycin-juried
auditory
HCs
SGNs.
results
showed
that
scaffolds
had
good
cytocompatibilities
mechanical
properties.
constructed
could
maintain
activity
well
vitro.
addition,
intervention
significantly
inhibit
neomycin-induced
HC
SGN
damage.
This
suggests
fabricated
highly
Corti,
which
may
provide
an
model
drug
development,
cell
gene
therapy
Biology,
Journal Year:
2024,
Volume and Issue:
13(10), P. 802 - 802
Published: Oct. 8, 2024
Inner
ear
disorders,
including
sensorineural
hearing
loss,
Meniere’s
disease,
and
vestibular
neuritis,
are
prevalent
conditions
that
significantly
impact
the
quality
of
life.
Despite
their
high
incidence,
underlying
pathophysiology
these
disorders
remains
elusive,
current
treatment
options
often
inadequate.
Emerging
evidence
suggests
pericytes,
a
type
vascular
mural
cell
specialized
to
maintain
integrity
function
microvasculature,
may
play
crucial
role
in
development
progression
inner
disorders.
The
pericytes
present
microvasculature
both
cochlea
system,
where
they
regulate
blood
flow,
blood–labyrinth
barrier,
facilitate
angiogenesis,
provide
trophic
support
neurons.
Understanding
valuable
insights
into
lead
novel
diagnostic
therapeutic
strategies,
improving
standard
living.
This
comprehensive
review
aims
detailed
overview
highlighting
anatomy
physiology
analyzing
mechanisms
contribute
Furthermore,
we
explore
potential
pericyte-targeted
therapies,
antioxidant,
anti-inflammatory,
angiogenic
approaches,
as
well
gene
therapy
strategies.
Frontiers in Cell and Developmental Biology,
Journal Year:
2022,
Volume and Issue:
10
Published: Dec. 13, 2022
Hearing
or
balance
loss
are
disabling
conditions
that
have
a
serious
impact
in
those
suffering
them,
especially
when
they
appear
children.
Their
ultimate
cause
is
frequently
the
of
function
mechanosensory
hair
cells
inner
ear.
Hair
can
be
damaged
by
environmental
insults,
like
noise
chemical
agents,
known
as
ototoxins.
Two
most
common
ototoxins
life-saving
medications:
cisplatin
against
solid
tumors,
and
aminoglycoside
antibiotics
to
treat
infections.
However,
due
their
localization
inside
temporal
bone,
difficult
study
mammals.
As
an
alternative
animal
model,
zebrafish
larvae
similar
mammals,
some
which
located
fish
specific
organ
on
surface
skin,
lateral
line.
This
makes
them
easy
observe
Development,
Journal Year:
2024,
Volume and Issue:
151(23)
Published: Nov. 29, 2024
ABSTRACT
Human
inner
ear
organoids
are
three-dimensional
tissular
structures
grown
in
vitro
that
recapitulate
some
aspects
of
the
fetal
and
allow
differentiation
cell
types.
These
offer
a
system
which
to
study
human
development,
mutations
causing
hearing
loss
vertigo,
new
therapeutic
drugs.
However,
extent
such
mimic
vivo
development
cellular
composition
remains
unclear.
Several
recent
studies
have
performed
single-cell
transcriptomics
on
interrogate
heterogeneity,
reveal
developmental
trajectories
sensory
lineages
compare
organoid-derived
vesicles
developing
ear.
Here,
we
discuss
insights
provided
by
these
analyses
help
define
paths
investigation
understand
development.
ABSTRACT
The
association
between
ear
and
kidney
anomalies
has
long
been
recognized.
However,
little
is
known
about
the
underlying
mechanisms.
In
last
two
decades,
embryonic
development
of
inner
studied
extensively.
Here,
we
describe
developmental
pathways
shared
both
organs
with
particular
emphasis
on
genes
that
regulate
signalling
cross
talk
specification
progenitor
cells
specialised
cell
types.
We
relate
this
to
clinical
features
oto-renal
syndromes
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