Unraveling the Regulatory Role of HuR/microRNA Axis in Colorectal Cancer Tumorigenesis DOI Open Access
Vikas Yadav, Tejveer Singh, Deepika Sharma

et al.

Cancers, Journal Year: 2024, Volume and Issue: 16(18), P. 3183 - 3183

Published: Sept. 18, 2024

Colorectal cancer (CRC) remains a significant global health burden with high incidence and mortality. MicroRNAs (miRNAs) are small non-protein coding transcripts, conserved throughout evolution, an important role in CRC tumorigenesis, either upregulated or downregulated various cancers. RNA-binding proteins (RBPs) known as essential regulators of miRNA activity. Human antigen R (HuR) is prominent RBP to drive tumorigenesis pivotal CRC. In this review, we discuss the regulatory HuR/miRNA axis Interestingly, miRNAs can directly target HuR, altering its expression However, HuR also stabilize degrade miRNAs, forming complex feedback loops that activate block CRC-associated signaling pathways. Dysregulation contributes initiation progression. Additionally, HuR-miRNA regulation by other non-coding RNAs, circular RNA (circRNAs), long-non-coding RNAs (lncRNAs) explored here. Understanding interplay could reveal novel biomarkers better diagnostic prognostic accuracy.

Language: Английский

Understanding the roles and regulation patterns of circRNA on its host gene in tumorigenesis and tumor progression DOI Creative Commons
Jianxia Wei, Mengna Li,

Changning Xue

et al.

Journal of Experimental & Clinical Cancer Research, Journal Year: 2023, Volume and Issue: 42(1)

Published: April 15, 2023

Abstract Circular RNAs (circRNAs) are a novel type of endogenous non-coding RNAs, which covalently closed loop structures formed by precursor mRNAs (pre-mRNAs) through back-splicing. CircRNAs abnormally expressed in many tumors, and play critical roles variety tumors as oncogenes or tumor suppressor genes sponging miRNAs, regulating alternative splicing transcription, cis-regulating host genes, interacting with RNA binding proteins (RBPs) encoding polypeptides. Among them, the regulation circRNAs on their corresponding is way for to exit functions. Accumulating evidence suggests that able regulate expression at transcriptional level, post-transcriptional translational post-translational Therefore, this paper mainly summarized association tumorigenesis progression, generalized function synergistically antagonistically elaborated mechanisms mutual between genes. More importantly, review provides specific references revealing potential application combined diagnosis, treatment prognosis.

Language: Английский

Citations

53
The logic of protein post‐translational modifications (PTMs): Chemistry, mechanisms and evolution of protein regulation through covalent attachments DOI Creative Commons
Marcin J. Suskiewicz

BioEssays, Journal Year: 2024, Volume and Issue: 46(3)

Published: Jan. 21, 2024

Abstract Protein post‐translational modifications (PTMs) play a crucial role in all cellular functions by regulating protein activity, interactions and half‐life. Despite the enormous diversity of modifications, various PTM systems show parallels their chemical catalytic underpinnings. Here, focussing on that involve addition new elements to amino‐acid sidechains, I describe historical milestones fundamental concepts support current understanding PTMs. The survey covers selected key research programmes, including study phosphorylation as regulatory switch, ubiquitylation degradation signal histone functional code. contribution techniques for studying PTMs is also discussed. central part essay explores shared principles strategies observed across diverse systems, together with mechanisms substrate selection, reversibility erasers recognition reader domains. Similarities basic mechanism are highlighted implications final dedicated evolutionary trajectories beginning possible emergence context rivalry prokaryotic world. Together, provides unified perspective world major modifications.

Language: Английский

Citations

9
Integration of multi-omics data accelerates molecular analysis of common wheat traits DOI Creative Commons
Ning Zhang, Li Tang, Songgang Li

et al.

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: March 5, 2025

Integration of multi-omics data can provide information on biomolecules from different layers to illustrate the complex biology systematically. Here, we build a atlas containing 132,570 transcripts, 44,473 proteins, 19,970 phosphoproteins, and 12,427 acetylproteins across wheat vegetative reproductive phases. Using this atlas, elucidate transcriptional regulation network, contributions post-translational modification (PTM) transcript level protein abundance, biased homoeolog expression PTM in wheat. The genes/proteins related development disease resistance are systematically analyzed, thus identifying phosphorylation and/or acetylation modifications for seed proteins controlling grain quality resistance-related genes. Lastly, unique module TaHDA9-TaP5CS1, specifying de-acetylation TaP5CS1 by TaHDA9, is discovered, which regulates Fusarium crown rot via increasing proline content. Our holds great promise fast-tracking molecular breeding studies crops.

Language: Английский

Citations

1
Monitoring Functional Posttranslational Modifications Using a Data‐Driven Proteome Informatic Pipeline DOI Creative Commons
Payman Nickchi, Uladzislau Vadadokhau, Mehdi Mirzaie

et al.

PROTEOMICS, Journal Year: 2025, Volume and Issue: unknown

Published: March 18, 2025

ABSTRACT Posttranslational modifications (PTMs) are of significant interest in molecular biomedicine due to their crucial role signal transduction across various cellular and organismal processes. Characterizing PTMs, distinguishing between functional inert modifications, quantifying occupancies, understanding PTM crosstalk challenging tasks any biosystem. Studying each often requires a specific, labor‐intensive experimental design. Here, we present PTM‐centric proteome informatic pipeline for predicting relevant PTMs mass spectrometry‐based proteomics data without prior information. Once predicted, these silico identified can be incorporated into refined database search compared measured data. As practical application, demonstrate how this used study glycoproteomics oral squamous cell carcinoma based on the profile primary tumors. Subsequently, experimentally proteins that differentially expressed cells treated with multikinase inhibitors dasatinib staurosporine using proteomics. Computational enrichment analysis was then employed determine potential induced by both drugs. Finally, conducted an additional round predicted PTMs. Our successfully analyzed enriched detected not initial search. findings support effectiveness searching MS computational analysis, propose integrating approach future engines.

Language: Английский

Citations

1
Regulation of insulin secretion by the post-translational modifications DOI Creative Commons
Chunhua Yang,

Mengna Wei,

Yanpu Zhao

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2023, Volume and Issue: 11

Published: Aug. 4, 2023

Post-translational modification (PTM) has a significant impact on cellular signaling and function regulation. In pancreatic β cells, PTMs are involved in insulin secretion, cell development, viability. The dysregulation of PTM cells is clinically associated with the development diabetes mellitus. Here, we summarized current findings major occurring their roles secretion. Our work provides comprehensive insight into understanding mechanisms secretion potential therapeutic targets for from perspective protein PTMs.

Language: Английский

Citations

12
Substrate and Functional Diversity of Protein Lysine Post-translational Modifications DOI Creative Commons
Bingbing Hao, Kaifeng Chen, Linhui Zhai

et al.

Genomics Proteomics & Bioinformatics, Journal Year: 2024, Volume and Issue: 22(1)

Published: Feb. 1, 2024

Abstract Lysine post-translational modifications (PTMs) are widespread and versatile protein PTMs that involved in diverse biological processes by regulating the fundamental functions of histone non-histone proteins. Dysregulation lysine is implicated many diseases, targeting PTM regulatory factors, including writers, erasers, readers, has become an effective strategy for disease therapy. The continuing development mass spectrometry (MS) technologies coupled with antibody-based affinity enrichment greatly promotes discovery decoding PTMs. global characterization crucial deciphering networks, molecular functions, mechanisms action In this review, we focus on PTMs, provide a summary enzymes proteomics advances MS technologies. We also discuss types crosstalks proteins current druggable targets factors

Language: Английский

Citations

4
Thirty years of molecular dynamics simulations on posttranslational modifications of proteins DOI
Austin T. Weigle, Jiangyan Feng, Diwakar Shukla

et al.

Physical Chemistry Chemical Physics, Journal Year: 2022, Volume and Issue: 24(43), P. 26371 - 26397

Published: Jan. 1, 2022

As of 2022, the protein structural effects induced by posttranslational modifications (PTMs) have been computationally studied for nearly 30 years. We review simulation PTMs given past and present state-of-the-art modeling analysis techniques.

Language: Английский

Citations

17
Understanding HAT1: A Comprehensive Review of Noncanonical Roles and Connection with Disease DOI Open Access
Miguel Á. Ortega,

Diego De León-Oliva,

Cielo García‐Montero

et al.

Genes, Journal Year: 2023, Volume and Issue: 14(4), P. 915 - 915

Published: April 14, 2023

Histone acetylation plays a vital role in organizing chromatin, regulating gene expression and controlling the cell cycle. The first histone acetyltransferase to be identified was 1 (HAT1), but it remains one of least understood acetyltransferases. HAT1 catalyzes newly synthesized H4 and, lesser extent, H2A cytoplasm. However, 20 min after assembly, histones lose marks. Moreover, new noncanonical functions have been described for HAT1, revealing its complexity complicating understanding functions. Recently discovered roles include facilitating translocation H3H4 dimer into nucleus, increasing stability DNA replication fork, replication-coupled chromatin coordination production, damage repair, telomeric silencing, epigenetic regulation nuclear lamina-associated heterochromatin, NF-κB response, succinyl transferase activity mitochondrial protein acetylation. In addition, levels linked many diseases, such as types cancer, viral infections (hepatitis B virus, human immunodeficiency virus viperin synthesis) inflammatory diseases (chronic obstructive pulmonary disease, atherosclerosis ischemic stroke). collective data reveal that is promising therapeutic target, novel approaches, RNA interference use aptamers, bisubstrate inhibitors small-molecule inhibitors, are being evaluated at preclinical level.

Language: Английский

Citations

10
The catalytic domain of free or ligand bound histone deacetylase 4 occurs in solution predominantly in closed conformation DOI Creative Commons

Markus Schweipert,

Thomas Nehls,

Anton Frühauf

et al.

Protein Science, Journal Year: 2024, Volume and Issue: 33(3)

Published: Feb. 15, 2024

Human histone deacetylase 4 (HDAC4) is a key epigenetic regulator involved in number of important cellular processes. This makes HDAC4 promising target for the treatment several cancers and neurodegenerative diseases, particular Huntington's disease. highly regulated by phosphorylation oxidation, which determine its nuclear or cytosolic localization, exerts function through multiple interactions with other proteins, forming multiprotein complexes varying composition. The catalytic domain known to interact SMRT/NCOR corepressor complex when structural zinc-binding (sZBD) intact forms closed conformation. Crystal structures have been reported showing an open conformation bound certain ligands. Here, we investigated relevance this under physiological conditions solution. We show that proper zinc chelation sZBD essential enzyme function. Loss ion not only leads massive decrease activity, but it also has serious consequences overall integrity stability protein. However, Zn

Language: Английский

Citations

4
USP34 regulates PIN1-cGAS-STING axis-dependent ferroptosis in cervical cancer via SUMOylation DOI
Dan Liao, Yumeng Cui,

Lijuan Shi

et al.

International Immunopharmacology, Journal Year: 2025, Volume and Issue: 147, P. 113968 - 113968

Published: Jan. 5, 2025

Language: Английский

Citations

0