Programmed DNA elimination DOI
Kazufumi Mochizuki

Current Biology, Journal Year: 2024, Volume and Issue: 34(18), P. R843 - R847

Published: Sept. 1, 2024

Language: Английский

Distinct satellite DNA composition between core and germline restricted chromosomes inBradysia (Sciara) coprophila DOI Creative Commons

Anne Kerrebrock,

Jullien M. Flynn, Robert B. Baird

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: April 16, 2025

Abstract Programmed DNA elimination (PDE), a phenomenon wherein cells eliminate subset of genetic material during certain stages development, is observed in broad range organisms. The fungus gnat Bradysia (formerly Sciara ) coprophila undergoes series PDE events their including germline-restricted chromosomes (called L chromosomes) soma and paternal male meiosis. However, the underlying mechanisms this are poorly understood. Highly repetitive satellite DNA, which often shows chromosome specific distribution, possible candidate for sequences involved PDE. In study, we utilized recent genomic data genome assemblies to identify new B. . Through characterization distribution on chromosomes, found that X autosomes do not share centromeric sequence with chromosomes. We further provide cytological evidence confirms finding based assembly there two distinct were previously distinguished cytologically. Together, our work lays foundation future studies explore connection between mechanism

Language: Английский

Citations

0
Multiplex DNA fluorescence in situ hybridization to analyze maternal vs. paternal C. elegans chromosomes DOI Creative Commons
Silvia Gutnik, Jia You, Ahilya N. Sawh

et al.

Genome biology, Journal Year: 2024, Volume and Issue: 25(1)

Published: March 14, 2024

Abstract Recent advances in microscopy have enabled studying chromosome organization at the single-molecule level, yet little is known about inherited organization. Here we adapt tracing to distinguish two C. elegans strains (N2 and HI) find that while their similar, N2 influences folding parameters of HI chromosome, particular step size, across generations. Furthermore, homologous chromosomes overlap frequently, but alignment between regions rare, suggesting transvection unlikely. We present a powerful tool investigate architecture track parent origin.

Language: Английский

Citations

2
PSRs: Selfish chromosomes that manipulate reproductive development DOI Creative Commons
Xinmi Zhang, Patrick M. Ferree

Seminars in Cell and Developmental Biology, Journal Year: 2024, Volume and Issue: 159-160, P. 66 - 73

Published: Feb. 22, 2024

Language: Английский

Citations

2
End resection and telomere healing of DNA double-strand breaks during nematode programmed DNA elimination DOI Creative Commons
Brandon Estrem, Richard E. Davis, Jianbin Wang

et al.

Nucleic Acids Research, Journal Year: 2024, Volume and Issue: 52(15), P. 8913 - 8929

Published: July 2, 2024

Abstract Most DNA double-strand breaks (DSBs) are harmful to genome integrity. However, some forms of DSBs essential biological processes, such as meiotic recombination and V(D)J recombination. also required for programmed elimination (PDE) in ciliates nematodes. In nematodes, the healed with telomere addition. While addition sites have been well characterized, little is known regarding that fragment nematode chromosomes. Here, we used embryos from human pig parasitic Ascaris characterize DSBs. Using END-seq, demonstrate introduced before mitosis, followed by extensive end resection. The resection profile unique each break site, generates 3′-overhangs neotelomeres. Interestingly, healing occurs much more frequently on retained DSB ends than eliminated ends. This biased repair may be due sequestration into micronuclei, preventing neotelomere formation at their Additional occur within both Parascaris, ensuring chromosomal breakage providing a fail-safe mechanism PDE. Overall, our data indicate specific responsible

Language: Английский

Citations

1
Essential roles of histone lysine methyltransferases EZH2 and EHMT1 in male embryo development of Phenacoccus solenopsis DOI Creative Commons
Haojie Tong, Mohamed A.A. Omar, Yuan Wang

et al.

Communications Biology, Journal Year: 2024, Volume and Issue: 7(1)

Published: Aug. 20, 2024

Paternal genome elimination (PGE) is an intriguing but poorly understood reproductive strategy in which females are typically diploid, males lose paternal genomes. heterochromatin (PGH) occurs arthropods with germline PGE, such as the mealybug, coffee borer beetles, and booklice. Here, we present evidence that PGH initially during early embryo development at around 15 h post-mating (hpm) cotton Phenacoccus solenopsis Tinsley. Transcriptome analysis followed by qPCR validation indicated six histone lysine methyltransferase (KMT) genes predominantly expressed adult females. We knocked down these five through dsRNA microinjection. found downregulation of two KMT genes, PsEZH2-X1 PsEHMT1, resulted a decrease heterochromatin-related methylations, including H3K27me1, H3K27me3, H3K9me3 ovaries, fewer male embryos, reduced offspring. For further confirmation, obtained strains transgenic tobacco highly expressing targeting respectively. Similarly, embryos offspring were observed when feeding on plants. Overall, PsEHMT1 have essential roles survival regulating formation mealybugs.

Language: Английский

Citations

1
Lack of paternal silencing and ecotype-specific expression in head and body lice hybrids DOI Creative Commons
Hollie Marshall, Andrés G. de la Filia,

R. Cavalieri

et al.

Evolution Letters, Journal Year: 2024, Volume and Issue: 8(3), P. 455 - 465

Published: Feb. 6, 2024

Paternal genome elimination (PGE) is a non-Mendelian inheritance system, described in numerous arthropod species, which males develop from fertilized eggs, but their paternally inherited chromosomes are eliminated before or during spermatogenesis. Therefore, PGE only transmit maternally set of to offspring. In addition the paternal chromosomes, diverse species have also repeatedly evolved transcriptional silencing genome, making effectively haploid. However, it unclear if this chromosome mechanistically linked has at later stage, and so, what drives haploidization under PGE. order understand these questions, here we study human louse,

Language: Английский

Citations

0
End resection and telomere healing of DNA double-strand breaks during nematode programmed DNA elimination DOI Creative Commons
Brandon Estrem, Richard E. Davis, Jianbin Wang

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: March 16, 2024

ABSTRACT Most DNA double-strand breaks (DSBs) are harmful to genome integrity. However, some forms of DSBs essential biological processes, such as meiotic recombination and V(D)J recombination. also required for programmed elimination (PDE) in ciliates nematodes. In nematodes, the healed with telomere addition. While addition sites have been well-characterized, little is known regarding that fragment nematode chromosomes. Here, we used embryos from Ascaris study timing PDE examine their end processing. Using END-seq, characterize DSB ends demonstrate introduced before mitosis, followed by extensive resection. The resection profile unique each break site, generates 3’ overhangs telomeres. Interestingly, healing occurs much more frequently on retained than eliminated ends. This biased repair may be due sequestration into micronuclei, preventing healing. Additional occur within both Parascaris , ensuring chromosomal breakage providing a fail-safe mechanism PDE.

Language: Английский

Citations

0
Programmed DNA elimination DOI
Kazufumi Mochizuki

Current Biology, Journal Year: 2024, Volume and Issue: 34(18), P. R843 - R847

Published: Sept. 1, 2024

Language: Английский

Citations

0