Single-cell multiome uncovers differences in glycogen metabolism underlying species-specific speed of development DOI Creative Commons
Alexandra de la Porte,

Julia Schröder,

Moritz Thomas

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 3, 2024

Abstract Embryos from different mammalian species develop at characteristic timescales. These timescales are recapitulated during the differentiation of pluripotent stem cells in vitro . Specific genes and molecular pathways that modulate cell speed between remain to be determined. Here we use single-cell multi-omic analysis neural mouse, cynomolgus human identify regulators for speed. We demonstrate species-specific transcriptome dynamics mirrored chromatin level, but is insensitive manipulations growth cycling. Exploiting resolution our data, glycogen storage levels regulated by UDP-glucose pyrophosphorylase 2 (UGP2) as a species-dependent trait cells, show lowered UGP2 mutant associated with accelerated differentiation. The control energy could general strategy regulation

Language: Английский

Compensation of gene dosage on the mammalian X DOI Creative Commons
Daniela Cecalev, Beatriz Viçoso, Rafael Galupa

et al.

Development, Journal Year: 2024, Volume and Issue: 151(15)

Published: Aug. 1, 2024

Changes in gene dosage can have tremendous evolutionary potential (e.g. whole-genome duplications), but without compensatory mechanisms, they also lead to dysregulation and pathologies. Sex chromosomes are a paradigmatic example of naturally occurring differences their compensation. In species with chromosome-based sex determination, individuals within the same population necessarily show 'natural' for chromosomes. this Review, we focus on mammalian X chromosome discuss recent new insights into dosage-compensation mechanisms that evolved along emergence chromosomes, namely X-inactivation X-upregulation. We evolution genetic loci molecular players involved, as well regulatory diversity potentially different requirements compensation across species.

Language: Английский

Citations

3

Ethical assessment of genome resource banking (GRB) in wildlife conservation DOI Creative Commons
Pierfrancesco Biasetti, Elena Mercugliano, Lisa Schrade

et al.

Cryobiology, Journal Year: 2024, Volume and Issue: 117, P. 104956 - 104956

Published: Sept. 13, 2024

Genome Resources Banks (GRBs) represent vital repositories for the systematic collection, storage, and management of genetic material across various taxa, with a primary objective safeguarding diversity research practical applications. Alongside development assisted reproductive techniques (ART), GRBs have evolved into indispensable tools in conservation, offering opportunities species preservation, mitigating inbreeding risks, facilitating fragmented populations. By preserving information suspended state, serve as backups against population vulnerabilities, potentially aiding restoration endangered extending their lifespan. While evidence demonstrates efficacy GRBs, ethical considerations surrounding biobanking procedures wildlife conservation remain largely unexplored. In this article, we will discuss possible issues related to need ethically monitor conservation. We then propose methodological tool, ETHAS, already use self-assessment reproduction techniques, assess also procedures. ETHAS can make it GRB from its design phase actual operation, helping build that meet high standards.

Language: Английский

Citations

0

Single-cell multiome uncovers differences in glycogen metabolism underlying species-specific speed of development DOI Creative Commons
Alexandra de la Porte,

Julia Schröder,

Moritz Thomas

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 3, 2024

Abstract Embryos from different mammalian species develop at characteristic timescales. These timescales are recapitulated during the differentiation of pluripotent stem cells in vitro . Specific genes and molecular pathways that modulate cell speed between remain to be determined. Here we use single-cell multi-omic analysis neural mouse, cynomolgus human identify regulators for speed. We demonstrate species-specific transcriptome dynamics mirrored chromatin level, but is insensitive manipulations growth cycling. Exploiting resolution our data, glycogen storage levels regulated by UDP-glucose pyrophosphorylase 2 (UGP2) as a species-dependent trait cells, show lowered UGP2 mutant associated with accelerated differentiation. The control energy could general strategy regulation

Language: Английский

Citations

0