Metabolic activities are selective modulators for individual segmentation clock processes DOI Creative Commons
Mitsuhiro Matsuda, Jorge Lázaro, Miki Ebisuya

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: June 6, 2024

Abstract A sequence of cellular and molecular processes unfolding during embryonic development prompts fundamental questions how the tempo multiple is coordinated whether a common global modulator exists. The oscillation segmentation clock well-studied model developmental tempo. While period known to scale with kinetics gene expression degradation core Hes7 across mammalian species, these key are remains unclear. In this study, we investigated if metabolic activities act as for clock, finding that they rather selective modulators. several inhibitions extended period, their effects on varied. Inhibition glycolysis decelerated protein production delay but did not influence intron delay. Electron transport chain inhibition without influencing other two processes. Combinations distinct exhibited synergistic effects. By contrast, temperature changes affected all three simultaneously. These results highlight processes, hinting scaled species may be achieved through combinations

Language: Английский

Metabolic activities are selective modulators for individual segmentation clock processes DOI Creative Commons
Mitsuhiro Matsuda, Jorge Lázaro, Miki Ebisuya

et al.

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: Jan. 20, 2025

Abstract Numerous cellular and molecular processes during embryonic development prompt the fundamental question of how their tempos are coordinated whether a common global modulator exists. While segmentation clock tempo scales with kinetics gene expression degradation core Hes7 across mammals, coordination these remains unclear. This study examines metabolic activities serve as for clock, finding them to be selective instead. Several inhibitions extend period but affect key differently: glycolysis inhibition slows protein production delay without altering intron delay, while electron transport chain extends influencing other processes. Combinations distinct exhibit synergistic effects. We propose that scaled species may result from combined modulators shaped by evolutionary constraints, rather than single modulator.

Language: Английский

Citations

0
Metabolic activities are selective modulators for individual segmentation clock processes DOI Creative Commons
Mitsuhiro Matsuda, Jorge Lázaro, Miki Ebisuya

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: June 6, 2024

Abstract A sequence of cellular and molecular processes unfolding during embryonic development prompts fundamental questions how the tempo multiple is coordinated whether a common global modulator exists. The oscillation segmentation clock well-studied model developmental tempo. While period known to scale with kinetics gene expression degradation core Hes7 across mammalian species, these key are remains unclear. In this study, we investigated if metabolic activities act as for clock, finding that they rather selective modulators. several inhibitions extended period, their effects on varied. Inhibition glycolysis decelerated protein production delay but did not influence intron delay. Electron transport chain inhibition without influencing other two processes. Combinations distinct exhibited synergistic effects. By contrast, temperature changes affected all three simultaneously. These results highlight processes, hinting scaled species may be achieved through combinations

Language: Английский

Citations

2