LGR4 (GPR48): The Emerging Inter-Bridge in Osteoimmunology

Biomedicines, Journal Year: 2025, Volume and Issue: 13(3), P. 607 - 607

Published: March 2, 2025

Leucine-rich repeat-containing G-protein-coupled receptor 4 (LGR4), a member of the (GPCR) family, has been implicated in various regulatory functions across multiple differentiation stages and numerous target sites bone diseases. Therefore, LGR4 is potential regulator nuclear factor-κB ligand (RANKL) during osteoclast differentiation. However, comprehensive investigation its applications immunology lacking. This review discusses molecular characteristics, signaling pathways, role osteoimmunology, with particular focus on interactions RANKL differentiation, while identifying gaps that warrant further research.

Language: Английский

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The conserved molecular mechanism of erectile dysfunction in type 2 diabetes rats and mice by cross-species transcriptomic comparisons

Sexual Medicine, Journal Year: 2025, Volume and Issue: 13(1)

Published: Feb. 1, 2025

The poor clinical situation of type 2 diabetes-induced erectile dysfunction (T2DMED) creates an urgent need for new therapeutic targets. To reveal the conserved molecular mechanism T2DMED across species. rat and mouse models were constructed to extract mRNA from corpus cavernosum high-throughput sequencing. differentially expressed genes (DEGs) analyzed Kyoto Encyclopedia Genes Genomes (KEGG), Gene Ontology (GO), Protein-Protein Interaction Networks performed by bioinformatics methods. Immunohistochemistry, immunofluorescence, hematoxylin- eosin Masson staining used subsequent verification. Cross-species transcriptomics rats mice validated. expression patterns in normal showed a strong correlation (r = 0.75, P < 2.2 × 10-16), with total 15 691 homologous identified. In both species, 553 down-regulated DEGs identified, mainly enriched pathways related smooth muscle mitochondrial functions, as revealed KEGG GO analyses. Immunohistochemistry immunofluorescence confirmed decreased α-smooth actin Uqcr10 tissues rats. Additionally, 239 up-regulated which Wnt signaling pathway extracellular matrix composition. Subsequent experiments increased β-catenin significant collagen accumulation, indicating fibrosis T2DMED. provide direction improving ability patients main strength is that cross-species transcriptomic sequencing has mechanisms limitation lack further validation patients. comparisons may offer novel strategy uncovering underlying identifying targets

Language: Английский

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Bioprospecting Marine Fungi from the Plastisphere: Osteogenic and Antiviral Activities of Fungal Extracts

Marine Drugs, Journal Year: 2025, Volume and Issue: 23(3), P. 115 - 115

Published: March 7, 2025

Marine microplastics (MPs) represent a novel ecological niche, populated by fungi with high potential for pharmaceutical discovery. This study explores the bioactivity of fungal strains isolated from MPs in Mediterranean sediments, focusing on their osteogenic and antiviral activities. Crude extracts prepared via solid-state submerged-state fermentation were tested effects extracellular matrix mineralization vitro bone growth zebrafish larvae, activity against respiratory syncytial virus (RSV) herpes simplex type 2 (HSV-2). Several exhibited significant mineralogenic activities, Aspergillus jensenii MUT6581 Cladosporium halotolerans MUT6558 being most performing ones. Antiviral assays identified A. Bjerkandera adusta MUT6589 as effective RSV HSV-2 at different extents, no cytotoxic effect. Although chemical profiling extract led to isolation decumbenones A B, they did not reproduce observed bioactivities, suggesting involvement other active compounds or synergistic effects. These results highlight plastisphere valuable resource bioactive suggest need further fractionation characterization identify molecules responsible these promising

Language: Английский

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Molecular architecture of human tooth development: A network-based analysis of gene expression

Human Gene, Journal Year: 2025, Volume and Issue: unknown, P. 201398 - 201398

Published: March 1, 2025

Language: Английский

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Non-coding RNAs in thoracic disease: Barrett’s esophagus and esophageal adenocarcinoma

Clinica Chimica Acta, Journal Year: 2025, Volume and Issue: unknown, P. 120242 - 120242

Published: March 1, 2025

Language: Английский

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Infection of human induced pluripotent stem cells by an oncogenic herpesvirus

Frontiers in Cellular and Infection Microbiology, Journal Year: 2025, Volume and Issue: 15

Published: March 13, 2025

Objective As one of the major human oncogenic viruses, Kaposi’s Sarcoma-associated Herpesvirus (KSHV) is closely related to several cancers such as sarcoma (KS) and primary effusion lymphoma (PEL). KSHV can infect a broad tropism cells in vitro vivo . Embryonic stem cell-like pluripotent be generated by simultaneous introduction factors, into somatic cells, yielding induced (iPS) cells. However, it remains unclear whether (hiPSCs) are permissive how this virus infection may affect cellular gene profile. Methods In current study, we examined hiPSCs were infection. The flow cytometry was used assess impacts on viability apoptosis. Illumina RNA-Sequencing determine profile changed KSHV-infected lytically Results We report that successfully establishes latent hiPSCs, which completely lytic reactivation release infectious virions. de novo arrests growth through inducing cell Transcriptomic analysis revealed significant changes global expression well Conclusion Our findings demonstrate powerful tool explore potential functions pathogenesis differentiated

Language: Английский

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Efficient Fabrication of Human Corneal Stromal Cell Spheroids and Promoting Cell Stemness Based on 3D-Printed Derived PDMS Microwell Platform

Biomolecules, Journal Year: 2025, Volume and Issue: 15(3), P. 438 - 438

Published: March 19, 2025

Spherical culture could promote the plasticity and stemness of human corneal stromal cells (hCSCs). Here, we introduce a novel three-dimensional (3D) cell system based on polydimethylsiloxane (PDMS) microwell platform composed many V-bottom microcavities to generate spheroids stemness. We isolated hCSCs from SMILE-derived lenticules maintained their physiological phenotype by culturing them in medium supplemented with extract (hCSE). Utilizing PDMS fabricated through 3D printing technology, successfully generated (3D-CSC) uniform size stable structure, exhibiting increased expression pluripotency factors, including OCT4, NANOG, SOX2, KLF4, PAX6. Furthermore, iPS supernatant E8-conditioned (E8-CM) significantly enhanced properties these cells. RNA sequencing proteomics analyses revealed that 3D-CSCs exhibited superior proliferation, differentiation, adhesion, migration, neurogenesis compared traditional monolayer cultures, underscoring role biophysical cues promoting In summary, this study presents an effective mimics vivo microenvironment, facilitating enhancement providing valuable insights into tissue engineering regenerative medicine, particularly for treating opacities diseases.

Language: Английский

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Anti-Tumor Potential of Frankincense Essential Oil and Its Nano-Formulation in Breast Cancer: An In Vivo and In Vitro Study

Pharmaceutics, Journal Year: 2025, Volume and Issue: 17(4), P. 426 - 426

Published: March 27, 2025

Background/Objective: Breast cancer remains the most common malignancy among women worldwide, contributing to high morbidity and mortality rates. Many anti-cancer drugs have been derived from medicinal plants, frankincense Boswellia carterii is notable for its anti-inflammatory, anti-neoplastic, anti-carcinogenic properties. Using gas chromatography/mass spectrometry (GC/MS), 48 components were identified in B. essential oil, major constituent was α-pinene (35.81%). Method: In this study, we investigated anti-tumor effects of oil (FEO) nano-formulation with chitosan (FEO-CSNPs) using vitro breast models (MCF-7, MDA-MB-231, 4T1 cells) vivo mouse mammary carcinoma (4T1) (Balb/c). Results: The results showed significant reductions cell viability. At 10 μg/mL, FEO highest reduction C-166 cells, while at 100 exhibited a stronger cytotoxicity MDA-MB-231 cells compared FEO-CSNPs CSNPs. growth arrest S, G2/M, G1/S phases MCF-7, lines (36.91%, 23.12%, 33.58%), addition increased apoptosis rates (33.04%, 36.39%, 42.19%). wound healing assays revealed decreased migratory ability treated cells. experiments balb/c mice demonstrated tumor volume, histopathological analysis confirming extensive necrosis. Moreover, antioxidant arginase activity. gene expression via qPCR indicated upregulation suppressor genes downregulation oncogenes. Conclusions: These findings suggest that nano-formulation, particularly form as an oral formulation, display enhanced efficacy, warranting further preclinical clinical research develop innovative treatment strategies.

Language: Английский

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Deciphering the interplay: circulating cell-free DNA, signaling pathways, and disease progression in idiopathic pulmonary fibrosis

3 Biotech, Journal Year: 2025, Volume and Issue: 15(4)

Published: March 29, 2025

Language: Английский

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Antioxidants in cancer therapy mitigating lipid peroxidation without compromising treatment through nanotechnology

Discover Nano, Journal Year: 2025, Volume and Issue: 20(1)

Published: April 24, 2025

Cancer treatments often exploit oxidative stress to selectively kill tumour cells by disrupting their lipid peroxidation membranes and inhibiting antioxidant enzymes. However, plays a dual role in cancer progression, acting as both promoter suppressor. Balancing through therapy remains challenge, excessive activity may compromise the efficacy of chemotherapy radiotherapy. This review explores antioxidants mitigating while maintaining treatment efficacy. It highlights recent advancements nanotechnology-based targeted delivery optimize therapeutic outcomes. A comprehensive literature was conducted using reputable databases, including PubMed, Scopus, Web Science, ScienceDirect. The search focused on publications from past five years (2020-2025), supplemented relevant studies earlier years. Keywords such "antioxidants," "lipid peroxidation," "nanotechnology therapy," "oxidative stress" were utilized. Relevant articles critically analysed, graphical illustrations created. Emerging evidence suggests that nanoparticles, liposomes, polymeric metal-organic frameworks, others, can effectively encapsulate control release minimizing systemic toxicity. Stimuli-responsive carriers with tumour-specific targeting mechanisms further enhance delivery. Studies indicate these strategies help preserve normal cells, mitigate stress-related damage, improve challenges bioavailability, stability, potential interactions standard therapies remain. Integrating nanotechnology antioxidant-based interventions presents promising approach for optimizing therapy. Future research should focus refining modulation strategies, assessing profiles during treatment, employing biomarkers determine optimal dosing. balanced use adverse effects.

Language: Английский

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