bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown
Published: Aug. 26, 2024
The steroid hormone aldosterone, produced by the zona glomerulosa (zG) of adrenal gland, is a master regulator plasma electrolytes and blood pressure. While aldosterone control renin-angiotensin system well understood, other key regulatory factors have remained elusive. Here, we replicated prior association between non-coding variant in
Language: Английский
Cell Death and Disease, Journal Year: 2025, Volume and Issue: 16(1)
Published: April 26, 2025
Abstract Chaperone proteins constitute a molecular machinery that controls proteostasis. HSPA2 is heat shock-non-inducible member of the human HSPA/HSP70 family, which includes several highly homologous chaperone proteins. exhibits cell type-specific expression pattern in testis, brain, and multilayered epithelia. It crucial male fertility-related factor, but its role somatic cells poorly understood. Previously, we found deficiency can impair epidermal keratinocyte differentiation. In this study, confirmed differentiation by investigating immortalized keratinocytes cultured reconstructed epidermis model. Moreover, uncovered influence on immunomodulation. Transcriptomic analysis revealed total loss affected genes related to interleukin- interferon-mediated signaling. The functional bidirectional changes associated with HSPA2. knockout HaCaT Ker-CT keratinocytes, not overproduction, impaired granular layer development as evidenced reduced levels late markers, filaggrin involucrin, along structural abnormalities upper layer. Differentiation defects were accompanied increased mRNA extracellular secretion keratinocyte-derived pro-inflammatory IL-6 cytokine CCL2, CCL8, CXCL1, CXCL6, CXCL10 chemokines. also led HSPA1 interferon-stimulated immune modulator SLAMF7. Knocking down decreased CCL5 release, suggesting HSPA1’s HSPA2-regulated network. To summarize, complex homeostatic keratinocytes. Our results suggest dysfunction activity could be an important pathogenicity factor potential therapeutic target for inflammatory cutaneous diseases.
Language: Английский
Citations
0
Phytomedicine, Journal Year: 2024, Volume and Issue: 132, P. 155810 - 155810
Published: June 8, 2024
Language: Английский
Citations
3
Published: Jan. 8, 2024
Background: Inhalation exposure to carcinogenic metals like cadmium (Cd) is a significant global health concern linked various cancers. However, understanding their precise mechanism remains elusive. Methods: Using the colon cancer (CC) rat model and implanted intestinal stent, exposed them atomized oxygen nitrogen (O2/N2) gas containing Cd. Results: Atmospheric Cd intensified inflammation in CC cells, heightening nicotinamide adenine dinucleotide phosphate 1 (NOX1) activity, generating increased reactive species (ROS). This escalated ROS production triggered abnormal Wnt protein secretion, activating Wnt/β-catenin pathway, stimulating cell proliferation. No discernible body weight effect was seen mice, possibly due later-stage tumor gain, masking changes. facilitated restructuring migration later. Intestinal stents inhibited superoxide dismutase (SOD1) mice's tumors, with no clear effects on NOX1, SOD2, catalase (CAT) enzymes. Elevated levels didn't substantially impact might slow its imbalance. Stent implantation eased inflammation, reducing proliferation but induced discomfort rats, food intake weight. Conclusion: partially foster tumorigenesis via ROS-mediated pathway. The of invasive cancerous not significant.
Language: Английский
Citations
2
Cancers, Journal Year: 2024, Volume and Issue: 16(16), P. 2861 - 2861
Published: Aug. 16, 2024
Cutaneous melanoma still represents a significant health burden worldwide, being responsible for the majority of skin cancer deaths. Key advances in therapeutic strategies have significantly improved patient outcomes; however, most patients experience drug resistance and tumor relapse. Cancer stem cells (CSCs) are small subpopulation different tumors, including melanoma, endowed with distinctive capacities self-renewal differentiation into bulk cells. Melanoma CSCs characterized by expression specific biomarkers intracellular pathways; moreover, they play pivotal role onset, progression resistance. In recent years, great efforts been made to dissect molecular mechanisms underlying protumor activities provide basis novel CSC-targeted therapies. Herein, we highlight intricate crosstalk between bystander microenvironment (TME), immune cells, endothelial cancer-associated fibroblasts (CAFs), its progression. Specifically, discuss peculiar escape host surveillance, recruit immunosuppressive educate toward an phenotype. We also address currently investigated that could pave way new promising approaches care.
Language: Английский
Citations
2
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown
Published: Aug. 26, 2024
The steroid hormone aldosterone, produced by the zona glomerulosa (zG) of adrenal gland, is a master regulator plasma electrolytes and blood pressure. While aldosterone control renin-angiotensin system well understood, other key regulatory factors have remained elusive. Here, we replicated prior association between non-coding variant in
Language: Английский
Citations
2