bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2024,
Volume and Issue:
unknown
Published: Nov. 12, 2024
The
blood-brain
barrier
(BBB)
is
a
highly
specialized
system
that
critical
for
regulating
transport
between
the
blood
and
central
nervous
system.
In
brain
tumors,
vasculature
compromised,
referred
to
as
blood-tumor
(BTB).
ability
precisely
model
unique
physiological
properties
of
BTB
essential
decipher
its
role
in
tumor
pathophysiology
rational
design
efficacious
therapeutics.
Here,
we
introduce
robust
high-throughput
vitro
3D
human
organoid
recapitulates
various
key
features
observed
vivo
clinical
GBM
samples.
organoids
are
composed
patient-derived
glioblastoma
stem
cells
(GSCs),
endothelial
(EC),
astrocytes
pericytes,
which
formed
through
self-assembly.
Transcriptomic
functional
analyses
reveal
GSCs
exhibit
enhanced
level
stemness,
mesenchymal
signature,
invasiveness
angiogenesis,
this
further
confirmed
studies.
We
demonstrate
therapeutic
delivery
drug
efficacy
on
cells.
Collectively,
our
findings
show
has
broad
utility
clinically
representative
studying
evaluating
therapies.
Genomics,
Journal Year:
2024,
Volume and Issue:
116(3), P. 110799 - 110799
Published: Jan. 28, 2024
Malignant
gliomas,
characterized
by
pronounced
heterogeneity,
a
complex
microenvironment,
and
propensity
for
relapse
drug
resistaniguree,
pose
significant
challenges
in
oncology.
This
study
aimed
to
investigate
the
prognostic
value
of
Ligand
Receptor
related
genes
(LRRGs)
within
glioma
microenvironment.
An
intersection
71
ligand-related
(LRGs)
2628
receptor-related
(RRGs)
yielded
total
69
LRRGs.
Utilizing
least
absolute
shrinkage
selection
operator
(LASSO)
regression
analysis,
RiskScore
model
comprising
28
LRRGs
was
constructed.
The
demonstrated
robust
value,
further
validated
TCGA-GBMLGG
dataset.
Subsequent
analyses
included
differential
gene
expression,
Gene
Ontology
(GO),
Kyoto
Encyclopedia
Genes
Genomes
(KEGG),
set
enrichment
(GSEA),
variation
(GSVA)
groups.
Additionally,
evaluations
PPI,
mRNA-RBP,
mRNA-TF,
mRNA-drug
interaction
networks
were
conducted.
Four
hub
identified
through
expression
analysis
across
various
GSE
datasets.
A
multivariate
Cox
constructed
nomogram
mutation
distribution.
underscores
role
intercellular
communication
microenvironment
identifies
four
crucial
assessment
clinical
patients.
These
findings
offer
potential
evaluation
framework
patients,
enhancing
our
understanding
disease
informing
future
therapeutic
strategies.
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2024,
Volume and Issue:
unknown
Published: Nov. 12, 2024
The
blood-brain
barrier
(BBB)
is
a
highly
specialized
system
that
critical
for
regulating
transport
between
the
blood
and
central
nervous
system.
In
brain
tumors,
vasculature
compromised,
referred
to
as
blood-tumor
(BTB).
ability
precisely
model
unique
physiological
properties
of
BTB
essential
decipher
its
role
in
tumor
pathophysiology
rational
design
efficacious
therapeutics.
Here,
we
introduce
robust
high-throughput
vitro
3D
human
organoid
recapitulates
various
key
features
observed
vivo
clinical
GBM
samples.
organoids
are
composed
patient-derived
glioblastoma
stem
cells
(GSCs),
endothelial
(EC),
astrocytes
pericytes,
which
formed
through
self-assembly.
Transcriptomic
functional
analyses
reveal
GSCs
exhibit
enhanced
level
stemness,
mesenchymal
signature,
invasiveness
angiogenesis,
this
further
confirmed
studies.
We
demonstrate
therapeutic
delivery
drug
efficacy
on
cells.
Collectively,
our
findings
show
has
broad
utility
clinically
representative
studying
evaluating
therapies.
