Theoretical analysis of low-power deep synergistic sono-optogenetic excitation of neurons by co-expressing light-sensitive and mechano-sensitive ion-channels

Communications Biology, Journal Year: 2025, Volume and Issue: 8(1)

Published: March 6, 2025

The present challenge in neuroscience is to non-invasively exercise low-power and high-fidelity control of neurons situated deep inside the brain. Although, two-photon optogenetic excitation can activate millimeter depth with sub-cellular specificity millisecond temporal resolution, it also cause heating targeted tissue. On other hand, sonogenetics modulate cellular activity expressed mechano-sensitive proteins deeper areas brain less spatial selectivity. We a theoretical analysis synergistic sono-optogenetic method overcome these limitations by co-expressing (MscL-I92L) ion-channel light-sensitive (CoChR/ChroME2s/ChRmine) hippocampal neurons. It shown that presence low-amplitude subthreshold ultrasound pulses, threshold for neural spiking reduces drastically 73% MscL-I92L-CoChR (0.021 mW/µm2), 66% MscL-I92L-ChroME2s (0.029 64% MscL-I92L-ChRmine (0.013 mW/µm2) at 5 Hz. allows up 1.2 cm combination. useful design new experiments multimodal neuroprosthetic devices circuits.

Language: Английский

Ultrasound‐Enabled Sonogenetics: Pioneering Advances in Cancer Theranostics

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 10, 2024

Abstract The diagnosis and treatment of cancer face significant challenges. Thus, precise controllable methods for effective tumor management are required. Sonogenetics which combines ultrasound technology with genetic engineering provides a promising solution. This approach utilizes the non‐invasive nature deep tissue penetration capabilities to enable diagnostics targeted therapies cancer. review explores roles sonogenetics‐mediated in theranostics. fundamental properties mechanisms underlying sonogenetics first introduced. Then, this work summarizes representative applications therapy, including cell‐based therapies, bacteria‐based eradication cells. Additionally, diagnosis, focusing on engineered gas vesicles (GVs) situ generation GVs diagnostic purposes presented. Finally, challenges clinical translation potential well discussed.

Language: Английский

Potential of ultrasound stimulation and sonogenetics in vision restoration: a narrative review

Neural Regeneration Research, Journal Year: 2024, Volume and Issue: 20(12), P. 3501 - 3516

Published: Dec. 16, 2024

Vision restoration presents a considerable challenge in the realm of regenerative medicine, while recent progress ultrasound stimulation has displayed potential as non-invasive therapeutic approach. This narrative review offers comprehensive overview current research on ultrasound-stimulated neuromodulation, emphasizing its treatment modality for various nerve injuries. By examining efficacy different types modulating peripheral and optic nerves, we can delve into their underlying molecular mechanisms. Furthermore, underscores sonogenetics vision restoration, which involves leveraging pharmacological genetic manipulations to inhibit or enhance expression related mechanosensitive channels, thereby strength response. We also address how methods such viral transcription be utilized render specific neurons organs highly responsive ultrasound, leading significantly improved outcomes.

Language: Английский