Research Square (Research Square),
Journal Year:
2024,
Volume and Issue:
unknown
Published: Feb. 27, 2024
Abstract
Epigenetic
patterns
including
DNA
methylation
are
known
to
vary
between
distantly
related
species,
but
it
is
not
clear
how
these
differ
at
an
intraspecific
level.
The
sweetpotato
whitefly,
Bemisia
tabaci
(Gennadius)
(Aleyrodidae;
Hemiptera),
encompasses
several
cryptic
species.
These
species
possess
highly
similar
genomes
exhibit
substantial
biological
and
physiological
differences.
B.
invasive,
polyphagous,
transmit
array
of
plant
infecting
single
stranded
viruses
(ssDNA)
–begomoviruses.
In
this
study,
around
genes
genomic
features
two
prominent
were
investigated
following
acquisition
a
monopartite
ssDNA
virus
–tomato
yellow
curl
virus.
included:
B
(also
as
Middle
East
Asia
Minor
1)
Q
Mediterranean).
Genomic
features,
such
promoters,
gene
bodies,
transposable
elements
assessed
for
levels
in
both
Differentially
methylated
regions
within
predominantly
unique
identified
respectively.
All
differentially
differential
expression
alternative
splicing
events
with
without
acquisition.
expressed
further
grouped
into
hyper-
hypomethylated
clusters.
clusters
included
implications
virus-vector
interactions
immune
functions
xenobiotics’
detoxification.
observed
pattern
differences
each
could,
part,
explain
some
the
them.
Animals,
Journal Year:
2025,
Volume and Issue:
15(2), P. 122 - 122
Published: Jan. 7, 2025
Monopterus
albus
is
a
protogynous
hermaphroditic
fish
that
changes
from
female
to
male,
but
the
underlying
sex
change
mechanism
remains
as-yet
unknown.
In
this
study,
we
firstly
cloned
and
characterized
sequence
protein
structure
of
unc-13d
M.
albus.
We
found
genomic
was
different
other
species.
Expression
detected
in
developing
gonad
by
applying
qRT-PCR
situ
hybridization.
expression
ovotestis
higher
than
ovary
testes.
A
strong
signal
oocytes
granulosa
cells
spermatogonia
primary
spermatocytes
promoter
methylation
negatively
correlated
with
gene
gonads,
especially
at
site
114.
dual-luciferase
assay
designed
revealed
dmrt1
regulates
activity
opposite
foxl2.
summary,
during
reversal,
DNA
affects
binding
transcription
factor
foxl2
region
through
demethylation
interactions
regulate
gonadal
development.
Genes,
Journal Year:
2025,
Volume and Issue:
16(5), P. 513 - 513
Published: April 28, 2025
Background/Objectives:
The
Pacific
oyster
Crassostrea
gigas
has
emerged
as
a
promising
model
system
for
sex
determination
studies
due
to
its
complex
reproduction
strategy
and
reversal.
Transcription
factors
(TFs)
play
crucial
roles
in
gonadal
differentiation.
Despite
previous
research
revealing
functions
of
several
conserved
sex-determining
pathway
genes,
such
Dmrt1,
Foxl2,
SoxH,
little
is
known
about
the
other
essential
TF
regulators
driving
C.
differentiation
development.
Methods:
In
this
study,
systematic
identification
TFs
revealed
1167
genes
genome.
Comparative
transcriptome
analysis
female
male
gonads
demonstrated
123
differentially
expressed
genes.
Results:
majority
these
sex-related
were
up-regulated
or
from
inactive
stage
mature
stage.
Moreover,
gene
set
was
deeply
showed
similar
regulation
Kumamoto
sikamea
gonads,
suggesting
their
important
regulatory
development
oysters.
Furthermore,
two
BTB
clusters
identified
genome,
both
which
specifically
gonad.
Gene
numbers
each
cluster
significant
variations
among
six
species.
Conclusions:
To
best
our
knowledge,
study
provides
first
report
whole
family
gigas.
will
be
valuable
resource
further
aimed
at
uncovering
networks
Genomics,
Journal Year:
2025,
Volume and Issue:
117(3), P. 111052 - 111052
Published: April 29, 2025
Estrogen
is
widely
distributed
in
the
aquatic
environment,
causing
feminization,
changes
of
gender
ratio
and
reduced
fecundity
organisms.
17β-estradiol
a
potent
estrogen
with
strong
estrogenic
activity
pseudo-persistence,
which
poses
significant
risks
to
global
ecosystems.
In
this
study,
effects
long-term
exposure
Manila
clam
(Ruditapes
philippinarum)
were
investigated.
The
clams
treated
(10
μg/L)
for
2
months
then
subjected
dissection.
Gonadal
tissue
sections
prepared
observation,
sex
was
calculated.
results
showed
that
female
male
control
group
0.93,
close
1.
Nevertheless,
17β-estradiol-treated
1.39,
nearly
50
%
higher
than
group.
Furthermore,
group,
no
hermaphrodites
observed.
However,
17β-estradiol,
them
hermaphroditic,
natural
environment
(approximately
one
thousandth).
It
implies
might
affect
sexual
differentiation
as
well
reversal
R.
Philippinarum,
promoting
individuals
reverse
into
individuals.
Comparative
transcriptomics
conducted
identify
genes
related
Philippinarum.
Gene
expression
profiles
estrogen-treated
females
(EF)
compared
those
males
(EM),
3751
differentially
expressed
(DEGs)
identified,
among
1512
up-regulated
females,
whereas
2239
males.
Meanwhile,
transcriptome
(DF)
(DM)
compared,
1718
601
upregulated
1117
GO
(gene
ontology)
KEGG
(Kyoto
encyclopedia
genomes)
analyses
above
mentioned
DEGs
identified
both
treatment
enriched
ubiquitination
pathway,
ribosome,
phagosome,
cytochrome
P450
metabolic
pathways.
noteworthy
some
gender-related
comparison
combination
DF
vs
DM
ubiquitination.
conculsion,
indicate
exogenous
estradiol
influence
enriching
knowledge
on
molecular
mechanisms
underlying
mollusks.
