Experimental Cell Research, Journal Year: 2024, Volume and Issue: unknown, P. 114388 - 114388
Published: Dec. 1, 2024
Language: Английский
Nature Reviews Urology, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 6, 2025
Language: Английский
Citations
0
Gene, Journal Year: 2025, Volume and Issue: unknown, P. 149355 - 149355
Published: Feb. 1, 2025
Language: Английский
Citations
0
BMC Genomics, Journal Year: 2025, Volume and Issue: 26(1)
Published: April 18, 2025
Hypoxia often has negative effects on testis development and spermatogenesis of mammals. Plateau yaks have lived in the hypoxia environment for generations, but ensured testicular function, which is closely related to their unique response mechanism. Glucose metabolic reprogramming an important way cells respond stressful environments, especially metabolite lactate, energy basis differentiation germ cells. In this study, (5% O2) effectively promoted yak Sertoli cell proliferation decreased autophagy apoptosis. It was found that showed good hypoxic adaptation. Metabolomics results glucose metabolism enhanced hypoxia, 13 metabolites were increased, including production transport level lactic acid (LA), may changed pentose phosphate pathway cells, these changes are conducive support balance under hypoxia. Crucially, when activated GLUT3, GLUT8, MCT4 proteins degraded, while GLUT1 MCT1 not affected, suggesting achieve by selectively regulating expression functional factors metabolism, intake yaks.
Language: Английский
Citations
0
Cells, Journal Year: 2024, Volume and Issue: 13(7), P. 592 - 592
Published: March 29, 2024
Preclinical research has provided compelling evidence indicating that exposure to hypobaric hypoxia (HH) results in a deterioration of spermatogenesis. This adverse effect extends the underlying molecular mechanisms, progressively leading impairments seminiferous epithelium and germ cells alterations semen parameters. Indeed, several studies have demonstrated animals exposed HH, whether natural high-altitude environments or under simulated hypoxic conditions, exhibit damage self-renewal differentiation spermatogenesis, an increase germline cell apoptosis, structural tubules. One primary mechanisms associated with inhibition apoptosis among is elevated level oxidative stress, which been closely HH exposure. Human shown individuals such as mountaineers alpinists, decreased sperm count, reduced motility, diminished viability, increased abnormal morphology their semen. strongly suggests may be considered significant risk factor could elevate prevalence male infertility. literature review aims provide comprehensive description propose potential elucidate infertility processes induced by HH. By doing so, it contributes expanding our understanding challenges posed extreme on human physiology, opening new avenues for this field.
Language: Английский
Citations
3
Biology, Journal Year: 2022, Volume and Issue: 11(12), P. 1791 - 1791
Published: Dec. 9, 2022
Sertoli cells play indispensable roles in spermatogenesis by providing the advanced germ with structural, nutritional, and regulatory support. Lactate is regarded as an essential Sertoli-cell-derived energy metabolite that nurses various types of spermatogenic cells; however, this assumption has not been tested using genetic approaches. Here, we have reported depletion lactate production conditionally deleting dehydrogenase A (Ldha) greatly affected spermatogenesis. Ldha deletion significantly reduced resulted severe defects Spermatogonia spermatocytes did show even mild impairments, but spermiogenesis conditional knockout males was severely disrupted. Further analysis revealed 2456 metabolites were altered sperm animals, specifically, lipid metabolism dysregulated, including choline, oleic acid, myristic acid. Surprisingly, choline supplementation completely rescued disorder caused loss activities. Collectively, these data demonstrated interruption impacted development through a choline-mediated mechanism. The outcomes findings novel function therapeutic applications treating human infertility.
Language: Английский
Citations
13
Journal of Advanced Research, Journal Year: 2023, Volume and Issue: 64, P. 15 - 30
Published: Nov. 11, 2023
Fetal hypoxia has long-term effects on postnatal reproductive functions and the mitochondrial impairments of ovarian granulosa cells may be one causes. Melatonin applied to mitigate dysfunction autophagy in mammalian been reported. However, potential mechanisms by which fetal damages function neonatal female mice melatonin this problem remain unclear.
Language: Английский
Citations
7
Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)
Published: Aug. 27, 2024
Yaks live in the Qinghai-Tibet Plateau for a long time where oxygen is scarce, but can ensure smooth development of testis and spermatogenesis. The key lies functional regulation Sertoli cells under hypoxia. In this study, we sequenced yak cultured normal concentration (Normoxia) treated with low (Hypoxia) by whole transcriptomics, screened out 194 differentially expressed mRNAs (DEmRNAs), 934 LncRNAs (DELncRNAs) 129 miRNAs (DEmiRNAs). GO KEGG analysis showed that these differential genes were mainly concentrated PI3K-AKT, MAPK, RAS, other signaling pathways, associated glucose metabolism, tight junction, steroid hormone synthesis, cell fusion, immunity cells. We constructed gene interaction network hypoxia relationship pairs related to metabolism junction. results suggested changes energy immune might provide favorable conditions This study provides data further on role non-coding RNA spermatogenesis yak.
Language: Английский
Citations
2
Cellular and Molecular Life Sciences, Journal Year: 2024, Volume and Issue: 81(1)
Published: May 9, 2024
Abstract Leydig cells are essential components of testicular interstitial tissue and serve as a primary source androgen in males. A functional deficiency often causes severe reproductive disorders; however, the transcriptional programs underlying fate decisions steroidogenesis these have not been fully defined. In this study, we report that homeodomain transcription factor PBX1 is master regulator cell differentiation testosterone production mice. was highly expressed peritubular myoid adult testis. Conditional deletion Pbx1 caused spermatogenic defects complete sterility. Histological examinations revealed impaired structure led to disorganization seminiferous tubules. Single-cell RNA-seq analysis loss function affected progenitor altered genes associated with synthesis directly regulates play important roles ( Prlr , Nr2f2 Nedd4 ). Further demonstrated leads significant decrease levels, accompanied by increases pregnenolone, androstenedione luteinizing hormone. Collectively, our data indispensable for maintaining function. These findings provide insights into dysgenesis regulation hormone secretion cells. Graphical abstract
Language: Английский
Citations
2
Science China Life Sciences, Journal Year: 2024, Volume and Issue: unknown
Published: Dec. 18, 2024
Language: Английский
Citations
2
iScience, Journal Year: 2023, Volume and Issue: 26(12), P. 108424 - 108424
Published: Nov. 9, 2023
In this study we explored the role of hypoxia and hypoxia-inducible transcription factor EPAS1 in regulating spermatogonial stem cell (SSC) function mouse testis. We have demonstrated that SSCs reside hypoxic microenvironments testis through utilization oxygen-sensing probe pimonidazole, by confirming stable presence EPAS1, which is degraded at >5% O2. Through generation a germline-specific Epas1 knockout line, modulation levels primary cultures spermatogonia with small drug molecule Daprodustat, required for robust SSC regenerative conditions (post-transplantation post-chemotherapy), via regulation key cellular processes such as metabolism. These findings shed light on relationship between male fertility will potentially facilitate optimization vitro culture infertility treatment pipelines using SSCs, those directed pediatric cancer survivors.
Language: Английский
Citations
4