MAD2L1-mediated NANOG nuclear translocation: A critical factor in lung cancer chemoresistance

Cellular Signalling, Journal Year: 2025, Volume and Issue: unknown, P. 111811 - 111811

Published: April 1, 2025

Language: Английский

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Reliable Harvest of Injectable Human Mesenchymal Stem Cell Sheets by Modulating Cell‐Substrate Adhesion Strength

Advanced Healthcare Materials, Journal Year: 2025, Volume and Issue: unknown

Published: April 18, 2025

Abstract Cell sheet engineering has emerged as a promising scaffold‐free strategy in cell‐based therapeutics, preserving essential cell‐cell and cell‐extracellular matrix (ECM) interactions. To enable minimally invasive delivery, key challenge relies on making the cell sheets compatible with injection‐based administration without subjecting sensitive cells to physical or thermal stresses. This study addresses reliable method for controlling dimensions by combining differential adhesion‐guided micropatterning along an isothermal detachment method. The surface composition of copolymer, poly(ethylene glycol dimethacrylate‐co‐hydroxyethyl methacrylate) is delicately controlled via initiated chemical vapor deposition ensure intact adhesion rapid under condition. optimized further allows hydrophobic microcontact printing creating micron‐sized sheets. Human mesenchymal stem harvested this show preserved ECM compromising viability after both injection. Moreover, injected substantially enhance angiogenic potential human umbilical vein endothelial cells, demonstrating sustained therapeutic activity It believed that approach great broaden scope engineering, serving robust platform regenerative medicine.

Language: Английский

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Expression of the stem cell markers NANOG and SOX2 in the cervical squamous carcinogenesis

Radiology and Oncology, Journal Year: 2025, Volume and Issue: unknown

Published: April 24, 2025

Abstract Background The aim of the present study was to assess a diagnostic potential stem cell markers NANOG and SOX2 for classifying cervical squamous intraepithelial lesions (SILs)/cervical neoplasia (CIN). Patients methods expression evaluated immunohistochemically on 40 patients: in 10 cases each low-grade SIL (LSIL), high-grade SIL/CIN, grade 2 (HSIL/CIN 2), HSIL/CIN, 3 3), carcinoma (CSCC) their adjacent non-dysplastic epithelium. In addition, human papillomavirus (HPV) genotyping immunohistochemical staining with p16 Ki-67 were done. compared between controls by multiplying intensity (SI) percentage positive cells (P) SI thickness epithelium (T) calculate x P T score. Results gradually increased from via LSIL HSIL CSCC. Expression higher (P < 0.05 Si scores score) lower all scores). HSIL/CIN showed than Conclusions Contrary p16, could be effective distinguishing changes. surrogate differentiating HSIL. Moreover, helpful 3. Further studies larger numbers patients molecular insights are needed.

Language: Английский

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SMAD2 linker phosphorylation impacts overall survival, proliferation, TGFβ1-dependent gene expression and pluripotency-related proteins in NSCLC

British Journal of Cancer, Journal Year: 2025, Volume and Issue: unknown

Published: May 3, 2025

Abstract Background We investigated the impact of SMAD2 linker phosphorylation (pSMAD2L) on overall and disease-free survival, signal transduction, as well cancer-related processes in non-small cell lung cancer (NSCLC). Methods generated A549 cells constitutively lacking pSMAD2L (A549L sub ) to gain mechanistic insights stimulated NSCLC lines with inhibitors against cycle-associated kinases or TGFβ1. In addition, we analysed alveolar epithelial type 2 from tumour-free tissue benign malignant T by Western blotting. Furthermore, pSMAD2L-positive tumours immune were an patient cohort ( n = 316) using multiplex immunofluorescence. Results cells, was expressed a mitosis-dependent manner. Loss had anti-proliferative effect, slowed migration, increased alternatively spliced short (SMAD2 ΔE3 ). The gene signature A549L associated developmental morphogenetic redirected canonical TGFβ1-dependent signalling. By contrast, absent but present lymphoblasts. patients low + tumour density poorer prognosis, whereas favoured survival. Conclusions antagonises TGFβ-signalling redirects expression, loss enhances affects pluripotency-associated proteins vitro. patients, influences survival spatially distinct

Language: Английский

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Combination therapies and novel delivery systems: a new frontier in overcoming TRAIL resistance in gastric cancer

Naunyn-Schmiedeberg s Archives of Pharmacology, Journal Year: 2025, Volume and Issue: unknown

Published: May 10, 2025

Language: Английский

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