Genome-wide Identification of the Laccase Gene Family in White Jute (Corchorus capsularis): Potential Targets for Lignin Engineering in Bast Fiber DOI Creative Commons

Subhadarshini Parida,

Deepak Kumar Jha,

Khushbu Kumari

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 19, 2024

Abstract The industrial bast fibre crop jute ( Corchorus sp.) is known for its long lignocellulosic multi-utility fibres. Information on lignification limited, and many enzymes in the lignin pathway are not well documented. One such enzyme laccase (EC 1.10.3.2), involved final polymerization step of lignification. A whole-genome search white capsularis ) revealed 34 putative CcaLAC genes. Phylogenetic analysis categorized these genes into six groups, with 17 predominantly expressed phloem tissue, 9 leaf, 4 xylem roots. steady increase gene expression, from plantlets to harvest, was observed several s. Some were selected further based homology Arabidopsis pathway-modifying laccases AtLAC s). Transcriptomics data confirmed their expression tissues, some showing significantly lower dlpf , a low-lignin fibre-containing mutant. Changes under abiotic stresses like ABA hormone copper heavy metal. Target sites Ath-miR397a Ath-miR397b predicted 11 s, respectively, suggesting possible post-transcriptional modification via microRNA. Subcellular localization showed s multiple plant cell compartments. Protein structure predictions up 10 motifs CcaLACs, 18 containing transmembrane helices. Overall, CcaLAC28 CcaLAC32 likely process (bast) jute. Modifying could enhance our understanding potentially lead development fibres, meeting high demands globally. Highlights Jute identified mainly tissue. key candidates Laccase changes stresses. Key Message Identified jute, engineering

Language: Английский

Harnessing the power of bacterial laccases for xenobiotic degradation in water: A 10-year overview DOI
Mujeeb Ur Rahman, Muhammad Wajid Ullah, Junaid Ali Shah

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 918, P. 170498 - 170498

Published: Feb. 1, 2024

Language: Английский

Citations

18
Research progress on the functions and biosynthesis of theaflavins DOI
Yufeng Liu,

Dongyang Wang,

Jing Li

et al.

Food Chemistry, Journal Year: 2024, Volume and Issue: 450, P. 139285 - 139285

Published: April 9, 2024

Language: Английский

Citations

8
Biochemical Characteristics of Laccases and Their Practical Application in the Removal of Xenobiotics from Water DOI Creative Commons
Agnieszka Gałązka, Urszula Jankiewicz, Andrzej Szczepkowski

et al.

Applied Sciences, Journal Year: 2023, Volume and Issue: 13(7), P. 4394 - 4394

Published: March 30, 2023

The rapid growth of the human population in recent decades has resulted intensive development various industries, urban agglomerations and increased production medicines for animals humans, plant protection products fertilizers on an unprecedented scale. Intensive agriculture, expanding areas newly established industrial plants release huge amounts pollutants into environment, which, nature, are very slowly degraded or not decomposed, which leads to their accumulation water terrestrial ecosystems. Researchers scouring extremely contaminated environments identify organisms that have ability degrade resistant xenobiotics, such as PAHs, some pharmaceuticals, plasticizers dyes. These a potential source enzymes could be used bioremediation municipal wastewater. Great hopes pinned oxidoreductases, including laccase, called by green biocatalyst because end product oxidation wide range substrates this enzyme is other compounds, most often dimers, trimers polymers. Laccase immobilization techniques use systems together with adsorption separation found application enzymatic

Language: Английский

Citations

16
miR397-LACs mediated cadmium stress tolerance in Arabidopsis thaliana DOI
Shahid Ali,

Shili Huang,

Jiajie Zhou

et al.

Plant Molecular Biology, Journal Year: 2023, Volume and Issue: 113(6), P. 415 - 430

Published: Aug. 11, 2023

Language: Английский

Citations

14
Unravelling the nexus of plant response to non-microbial biostimulants under stress conditions DOI Creative Commons
Michele Ciriello, Giovanna Marta Fusco, Pasqualina Woodrow

et al.

Plant Stress, Journal Year: 2024, Volume and Issue: 11, P. 100421 - 100421

Published: March 1, 2024

Contemporary challenges facing the agricultural sector have garnered interest of all stakeholders on novel toolset biostimulants. These products could serve as pivotal actors in forthcoming transition toward ever more essential sustainable production practices. Regardless their type, biostimulants potential to enhance resource efficiency while concurrently fortifying plant resilience adverse abiotic stress factors. Recent research advances fundamentally focused assessing quantifiable parameters, largely overlooking numerous and intricate biochemical, cellular, metabolic interactions between plants It is consequently not surprising that, date, mechanisms action basic biochemical processes underlying biostimulants' effects remain enigmatic. Concerning non-microbial biostimulants, which are subject in-depth exploration this review, inherently diverse nature, comprising formulations containing a plethora distinct bioactive molecules, significantly complicates investigation implicated mode action. for reason that we rather elected meticulously examine effects, particularly suboptimal environments, a) protein hydrolysates; b) algal extracts; c) humic acids; d) silicon. The objective analysis gain comprehensive understanding how these substances operate within by interpreting both genetic impacts. Comprehensive substantially underpin reliability agents usher identification effective formulations.

Language: Английский

Citations

6
Exploring laccase: a sustainable enzymatic solution for the paper recycling domain DOI
Sheetal Pandey, Sarika Gupta

Archives of Microbiology, Journal Year: 2024, Volume and Issue: 206(5)

Published: April 11, 2024

Language: Английский

Citations

6
The Laccase Family Gene CsLAC37 Participates in Resistance to Colletotrichum gloeosporioides Infection in Tea Plants DOI Creative Commons

Dangqiang Li,

Hongxiu Zhang,

Qianqian Zhou

et al.

Plants, Journal Year: 2024, Volume and Issue: 13(6), P. 884 - 884

Published: March 19, 2024

Fungal attacks have become a major obstacle in tea plantations. Colletotrichum gloeosporioides is one of the most devastating fungal pathogens plantations that can severely affect yield and quality. However, molecular mechanism resistance genes involved anthracnose still largely unknown plants. Here, we found laccase gene CsLAC37 was response to infection based on transcriptome analysis. The full-length CDS cloned, its protein sequence had closest relationship with Arabidopsis AtLAC15 compared other AtLACs. Tissue-specific expression analysis showed higher levels mature leaves stems than tissues. Subcellular localization predominantly localized cell membrane. were upregulated at different time points under cold, salt, SA, ABA treatments. qRT-PCR confirmed responded both Pestalotiopsis-like species C. infections. Functional validation hydrogen peroxide (H2O2) content increased significantly, POD activity decreased after antisense oligonucleotide (AsODN) treatment controls. results demonstrated may play an important role anthracnose, findings provide theoretical foundation for breeding varieties diseases.

Language: Английский

Citations

5
The Analysis, Description, and Examination of the Maize LAC Gene Family’s Reaction to Abiotic and Biotic Stress DOI Open Access

Tonghan Wang,

Yang Liu,

Kunliang Zou

et al.

Genes, Journal Year: 2024, Volume and Issue: 15(6), P. 749 - 749

Published: June 6, 2024

Laccase (LAC) is a diverse group of genes found throughout the plant genome essential for growth and response to stress by converting monolignin into intricate lignin formations. However, comprehensive investigation maize laccase has not yet been documented. A bioinformatics approach was utilized in this research conduct thorough examination (

Language: Английский

Citations

4
Changes in hemicellulose metabolism in banana peel during fruit development and ripening DOI

Zan Yi,

Rahat Sharif,

Shazma Gulzar

et al.

Plant Physiology and Biochemistry, Journal Year: 2024, Volume and Issue: 215, P. 109025 - 109025

Published: Aug. 8, 2024

Language: Английский

Citations

4
Genome-wide associations of sweetpotato metabolites enhance genomic prediction and identify genes in metabolic and regulatory pathways DOI Creative Commons

Tara Rickman,

Alison K. Adams, Phillip A. Wadl

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: March 20, 2025

Global sweetpotato production is increasing due to its health benefits, including high levels of complex carbohydrates and bioactive compounds. To explore the genetic basis carotenoids, we conducted a genome-wide association study (GWAS) using diverse accessions, two decades phenotypic data, 252,975 dosage-based SNPs INDELs. Our findings confirmed negative correlation between dry matter β-carotene identified interconnected metabolic pathways regulating multiple traits. Notably, phytoene synthase, involved in carotene biosynthesis, was associated with matter. Other linked these traits include carbohydrate metabolism, cell wall modification, phosphate starvation, stress response, flowering regulation. evaluate breeding potential GWAS-assisted genomic prediction (GWABLUP), found that 500 top GWAS hits used for significantly enhanced predictive ability (PA) six out nine traits, improving PA by up 6.7% 15.9% compared Genomic Best Linear Unbiased Prediction (GBLUP), which utilized 41,551 markers, respectively. The best across ranged from 20.9% 60.6%, both additive dominance effects playing an important role. Model selection, guided resample model inclusion probability (RMIP), during GWABLUP after each iteration typically yielded highest PA. These results provide valuable insights strategies aimed at optimizing agronomic addressing market demands value-added products.

Language: Английский

Citations

0