Role of m6A Methylation Regulators in the Diagnosis and Subtype Classification of COPD Based on the GEO Database DOI Creative Commons

Ping-An Zhang,

Na Gao, Xiaoning Li

et al.

Journal of Cellular and Molecular Medicine, Journal Year: 2024, Volume and Issue: 28(22)

Published: Nov. 1, 2024

ABSTRACT N6‐methyladenosine (m6A) is a prevalent mRNA modifier, yet its role in chronic obstructive pulmonary disease (COPD) remains unexplored. We sourced expression levels of m6A methylation regulators from the GSE76925 dataset. These regulators' differential (DEMs) predicted COPD risk via random forest and support vector machine models. Additionally, nomogram model using DEMs estimated prevalence. employed consistent cluster analysis to categorise samples into distinct subtypes. Analyses immune cell infiltration these subtypes gene (DEGs) across were conducted. A validated several their associated pathways. Fifteen showed used Eleven selected for model, which decision curve suggested could benefit patients. Consensus divided two subtypes: Cluster B. B was with neutrophil eosinophil‐dominated immunity, while linked monocyte‐dominated immunity. Validation some research findings achieved through experiments. appear instrumental diagnosing classifying COPD.

Language: Английский

Role of m6A Methylation Regulators in the Diagnosis and Subtype Classification of COPD Based on the GEO Database DOI Creative Commons

Ping-An Zhang,

Na Gao, Xiaoning Li

et al.

Journal of Cellular and Molecular Medicine, Journal Year: 2024, Volume and Issue: 28(22)

Published: Nov. 1, 2024

ABSTRACT N6‐methyladenosine (m6A) is a prevalent mRNA modifier, yet its role in chronic obstructive pulmonary disease (COPD) remains unexplored. We sourced expression levels of m6A methylation regulators from the GSE76925 dataset. These regulators' differential (DEMs) predicted COPD risk via random forest and support vector machine models. Additionally, nomogram model using DEMs estimated prevalence. employed consistent cluster analysis to categorise samples into distinct subtypes. Analyses immune cell infiltration these subtypes gene (DEGs) across were conducted. A validated several their associated pathways. Fifteen showed used Eleven selected for model, which decision curve suggested could benefit patients. Consensus divided two subtypes: Cluster B. B was with neutrophil eosinophil‐dominated immunity, while linked monocyte‐dominated immunity. Validation some research findings achieved through experiments. appear instrumental diagnosing classifying COPD.

Language: Английский

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