A Lateral Line Specific Mucin Involved in Cupula Growth and Vibration Detection in Zebrafish DOI Open Access
Ziyue Ma,

Yixuan Tian,

Yingying Wang

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(2), P. 708 - 708

Published: Jan. 15, 2025

The lateral line system in fish is crucial for detecting water flow, which facilitates various behaviors such as prey detection, predator avoidance, and rheotaxis. cupula, a gelatinous structure overlaying the hair cells neuromasts, plays key role transmitting mechanical stimuli to cells. However, molecular composition of cupula matrix remains poorly understood. In this study, we found that Mucin-5AC, novel family mucin proteins, composed 2–27 cysteine-rich domains, presents cartilaginous bony fishes. Using situ hybridization transgenic reporter assays, demonstrated zebrafish muc5AC specifically expressed support neuromasts. Knockdown via antisense morpholino resulted shorter cupulae line. Additionally, generated mutants using CRISPR/Cas9 were significantly than wild-types, but cell number neuromasts was not changed obviously. Furthermore, mutant larvae displayed compromised sensitivity vibration compared wild-type larvae. This study provides first evidence linking gene development detection zebrafish. Our findings suggest Mucin-5AC likely critical component matrix, offering an important clue fish.

Language: Английский

A Lateral Line Specific Mucin Involved in Cupula Growth and Vibration Detection in Zebrafish DOI Open Access
Ziyue Ma,

Yixuan Tian,

Yingying Wang

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(2), P. 708 - 708

Published: Jan. 15, 2025

The lateral line system in fish is crucial for detecting water flow, which facilitates various behaviors such as prey detection, predator avoidance, and rheotaxis. cupula, a gelatinous structure overlaying the hair cells neuromasts, plays key role transmitting mechanical stimuli to cells. However, molecular composition of cupula matrix remains poorly understood. In this study, we found that Mucin-5AC, novel family mucin proteins, composed 2–27 cysteine-rich domains, presents cartilaginous bony fishes. Using situ hybridization transgenic reporter assays, demonstrated zebrafish muc5AC specifically expressed support neuromasts. Knockdown via antisense morpholino resulted shorter cupulae line. Additionally, generated mutants using CRISPR/Cas9 were significantly than wild-types, but cell number neuromasts was not changed obviously. Furthermore, mutant larvae displayed compromised sensitivity vibration compared wild-type larvae. This study provides first evidence linking gene development detection zebrafish. Our findings suggest Mucin-5AC likely critical component matrix, offering an important clue fish.

Language: Английский

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