Investigating the Variation between Lignin Content and the Fracture Characteristics in Capsicum annuum Mutant Stems DOI Creative Commons
Bánk Pápai, Zsófia Kovács, Zoltán Tóth

et al.

Agriculture, Journal Year: 2024, Volume and Issue: 14(10), P. 1771 - 1771

Published: Oct. 8, 2024

The cultivation of horticultural plants in controlled greenhouse environments is a pivotal practice modern agriculture, offering the potential to enhance crop productivity and mitigate climate change effects. This study investigates biomechanical properties lignin content various Capsicum annuum mutant lines—‘fragile-plant’ (frx), ‘tortuous internodi’ (tti), ‘puffy-structured stem’ (pfi)—in comparison commercially established variety, ‘Garai Fehér’. We employed acetyl bromide method quantify conducted three-point bending tests assess rigidity three distinct regions stem. Gene expression analysis key biosynthetic pathway genes (PAL, C4H, 4CL, CCoAOMT, CAD) was performed using qRT-PCR. results revealed significant differences breaking force among genotypes stem regions. tti mutants exhibited similar control but lower strength, likely due elongated internodes. frx showed uniformly reduced content, correlating with their fragile stems. pfi displayed abnormally high top region yet demonstrated lowest every region. Overexpression CAD CCoAOMT detected specific stem, suggesting alterations biosynthesis; however, we could not confirm correlation between them. Our findings indicate that while generally correlates rigidity, this trait complex influenced by more factors.

Language: Английский

Integrated Transcriptomic and Targeted Metabolomic Analysis Reveals the Key Genes Involved in Triterpenoid Biosynthesis of Ganoderma lucidum DOI Creative Commons
Xiaolan Xu, Chunxia Li, Feilun Wu

et al.

Journal of Fungi, Journal Year: 2025, Volume and Issue: 11(1), P. 57 - 57

Published: Jan. 13, 2025

Ganoderma lucidum is a traditional Chinese medicinal fungus, and ganoderma triterpenoids (GTs) are one of the main bioactive compounds. These compounds have various pharmacological functions, including anti-tumor, antioxidant, anti-inflammatory, liver-protective, immune-regulating effects. However, manner in which they accumulate, their biosynthesis mechanisms remain unclear. To screen for genes that involved biosynthetic pathway GTs, this study analyzed differential metabolites differentially expressed (DEGs) among different growth stages G. lucidum, primordia (P), matured fruiting body (FM), post-spore bodies (FP) using targeted metabolomics transcriptomics analysis, respectively. The results showed total 699 components were detected, lignans, terpenoids, amino acids derivatives, phenolic acids, others. Among them, 112 types triterpenes detected. Compared with primordia, there eight triterpenoids, three decreasing five increasing FM stage. A comparison between stage FP revealed 13 triterpenoids. analysis 371 DEGs P_vs_FM group, 171 down-regulated 200 up-regulated genes. In FM_vs_FP 2567 identified, 1278 1289 Through transcriptome correlation six TFs two CYP450s significantly associated four triterpenoid components. these positively or negatively correlated addition, interestingly, some flavonoids compounds, been reported plants, also detected indicating it has potential to be engineered into strain capable synthesizing flavonoid This provides useful information about key GT biosynthesis, but further exploration in-depth research needed better understand functions

Language: Английский

Citations

1
Calcium chloride enhances phenylpropanoid metabolism, antioxidant ability and phytohormone signaling to effectively alleviate chilling injury in postharvest nectarines DOI
Yudong Liu, Jinlong Wu, Yong Li

et al.

Postharvest Biology and Technology, Journal Year: 2024, Volume and Issue: 217, P. 113122 - 113122

Published: July 26, 2024

Language: Английский

Citations

5
Combined transcriptomic and metabolomic analyses reveal the pharmacognostic mechanism of the metabolism of flavonoids in different parts of Polygonum capitatum DOI Creative Commons
Jie Yang, Yu Zhang,

Bu‐Fa Guo

et al.

The Plant Genome, Journal Year: 2025, Volume and Issue: 18(1)

Published: Jan. 14, 2025

Abstract The plant Polygonum capitatum ( P. ) contains a variety of flavonoids that are distributed differently among different parts. Nevertheless, differentially expressed genes (DEGs) associated with this heterogeneous distribution have not been identified. In study, combined transcriptomic and metabonomic analysis, we identified significant DEGs related to variations in flavonoid composition parts . Subsequently, nontargeted metabolomic analyses revealed phenolic acids were significantly enriched the phenylpropanoid biosynthesis, shikimic acid biosynthesis pathways. expression levels encoding enzymes, including shikimate O ‐hydroxycinnamoyltransferase (HCT), chalcone synthase (CHS), 3′,5′‐hydroxylase (CYP75A), flavones 3‐hydroxylase (F3H), flavonol (FLS), leucoanthocyanidin reductase (LAR), trans‐cinnamate 4‐monooxygenase (CYP73A), kinase (SK), found be lowest leaves via quantitative PCR. Interestingly, these involved quality markers such as gallic acid, quercetin, quercitrin Finally, targeted results reconfirmed contents highest This research provides theoretical basis for further understanding differential regulatory mechanism metabolism , providing novel insights into pharmacognostic

Language: Английский

Citations

0
Genome-Wide Analysis of the 4-Coumarate-CoA Ligase Gene Family in Wheat: Expression Patterns under Salt Stress and Light-Regulated Cold Acclimation DOI Creative Commons
Fatemeh Gholizadeh, Gabriella Szalai, Tibor Janda

et al.

Plant Stress, Journal Year: 2025, Volume and Issue: 16, P. 100879 - 100879

Published: May 5, 2025

Language: Английский

Citations

0
NtWIN1 regulates the biosynthesis of scopoletin and chlorogenic acid by targeting NtF6’H1 and NtCCoAMT genes in Nicotiana tabacum DOI

Shun He,

Junping Gao, Bingyu Li

et al.

Plant Physiology and Biochemistry, Journal Year: 2024, Volume and Issue: 214, P. 108937 - 108937

Published: July 14, 2024

Language: Английский

Citations

3
Investigating the Variation between Lignin Content and the Fracture Characteristics in Capsicum annuum Mutant Stems DOI Creative Commons
Bánk Pápai, Zsófia Kovács, Zoltán Tóth

et al.

Agriculture, Journal Year: 2024, Volume and Issue: 14(10), P. 1771 - 1771

Published: Oct. 8, 2024

The cultivation of horticultural plants in controlled greenhouse environments is a pivotal practice modern agriculture, offering the potential to enhance crop productivity and mitigate climate change effects. This study investigates biomechanical properties lignin content various Capsicum annuum mutant lines—‘fragile-plant’ (frx), ‘tortuous internodi’ (tti), ‘puffy-structured stem’ (pfi)—in comparison commercially established variety, ‘Garai Fehér’. We employed acetyl bromide method quantify conducted three-point bending tests assess rigidity three distinct regions stem. Gene expression analysis key biosynthetic pathway genes (PAL, C4H, 4CL, CCoAOMT, CAD) was performed using qRT-PCR. results revealed significant differences breaking force among genotypes stem regions. tti mutants exhibited similar control but lower strength, likely due elongated internodes. frx showed uniformly reduced content, correlating with their fragile stems. pfi displayed abnormally high top region yet demonstrated lowest every region. Overexpression CAD CCoAOMT detected specific stem, suggesting alterations biosynthesis; however, we could not confirm correlation between them. Our findings indicate that while generally correlates rigidity, this trait complex influenced by more factors.

Language: Английский

Citations

0