Differential Cytotoxicity of Surface-Functionalized Silver Nanoparticles in Colorectal Cancer and Ex-Vivo Healthy Colonocyte Models DOI Open Access
Marianna Barbalinardo, Emilia Benvenuti, Denis Gentili

et al.

Cancers, Journal Year: 2025, Volume and Issue: 17(9), P. 1475 - 1475

Published: April 27, 2025

Background/Objectives: Engineered nanomaterials, particularly silver nanoparticles (AgNPs), have emerged as promising tools in oncology due to their ability enhance tumor targeting and minimize off-target effects. This study investigates the cytotoxic effects of two different types AgNPs-citrate-coated (AgNPs-cit) EG6OH-coated (AgNPs-EG6OH)-on colorectal cancer (CRC) cell lines healthy colonocytes, aiming assess potential selective therapeutic agents. Methods: AgNPs-cit AgNPs-EG6OH were synthesized characterized for size surface properties. LoVo (microsatellite instability-high) HT-29 stable) CRC lines, along with primary colonocyte cultures from mucosal tissues, exposed these nanoparticles. Cytotoxicity was assessed through MTT assays, while morphological changes observed using fluorescence microscopy. Internalization evaluated by confocal Results: exhibited significant cytotoxicity cells, reducing viability inducing indicative programmed death, especially after 48 h exposure. In contrast, showed minimal on cells no toxicity or colonocytes. Confocal microscopy confirmed nanoparticle internalization, functionalization influencing distribution patterns within cells. Conclusions: demonstrates that significantly influences AgNPs, citrate-coated showing microsatellite instability-high These findings underscore surface-modified targeted therapy highlight importance tailoring design optimize efficacy minimizing

Language: Английский

Differential Cytotoxicity of Surface-Functionalized Silver Nanoparticles in Colorectal Cancer and Ex-Vivo Healthy Colonocyte Models DOI Open Access
Marianna Barbalinardo, Emilia Benvenuti, Denis Gentili

et al.

Cancers, Journal Year: 2025, Volume and Issue: 17(9), P. 1475 - 1475

Published: April 27, 2025

Background/Objectives: Engineered nanomaterials, particularly silver nanoparticles (AgNPs), have emerged as promising tools in oncology due to their ability enhance tumor targeting and minimize off-target effects. This study investigates the cytotoxic effects of two different types AgNPs-citrate-coated (AgNPs-cit) EG6OH-coated (AgNPs-EG6OH)-on colorectal cancer (CRC) cell lines healthy colonocytes, aiming assess potential selective therapeutic agents. Methods: AgNPs-cit AgNPs-EG6OH were synthesized characterized for size surface properties. LoVo (microsatellite instability-high) HT-29 stable) CRC lines, along with primary colonocyte cultures from mucosal tissues, exposed these nanoparticles. Cytotoxicity was assessed through MTT assays, while morphological changes observed using fluorescence microscopy. Internalization evaluated by confocal Results: exhibited significant cytotoxicity cells, reducing viability inducing indicative programmed death, especially after 48 h exposure. In contrast, showed minimal on cells no toxicity or colonocytes. Confocal microscopy confirmed nanoparticle internalization, functionalization influencing distribution patterns within cells. Conclusions: demonstrates that significantly influences AgNPs, citrate-coated showing microsatellite instability-high These findings underscore surface-modified targeted therapy highlight importance tailoring design optimize efficacy minimizing

Language: Английский

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