Genetic Screening Reveals Cone Cell-Specific Factors as Common Genetic Targets Modulating Rival-Induced Prolonged Mating in male Drosophila melanogaster DOI Creative Commons
Yanying Sun, Xiaoli Zhang, Zekun Wu

et al.

G3 Genes Genomes Genetics, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 4, 2024

Abstract Male-male social interactions exert a substantial impact on the transcriptional regulation of genes associated with aggression and mating behavior in male Drosophila melanogaster. Throughout our comprehensive genetic screening aggression-related genes, we identified that majority mutants for these are rival-induced visually-oriented behavior, longer-mating-duration (LMD). The upregulated single-housed males significantly altered LMD but not copulation latency, suggesting primary duration. Single-cell RNA sequencing revealed LMD-related predominantly co-expressed male-specific like dsx Cyp6a20 specific cell populations, especially cone cells. Functional validation confirmed roles mediating LMD. Expression Cyp6a20, Cyp4d21, CrzR was enriched cells, disruptions cell-specific expression Cyp4d21 leading to disrupted We also novel gene, CG10026/Macewindu, reversed when overexpressed These findings underscore critical role cells as pivotal site involved behavior. This study provides valuable insights into intricate mechanisms underlying complex sexual behaviors Drosophila.

Language: Английский

Genetic Screening Reveals Cone Cell-Specific Factors as Common Genetic Targets Modulating Rival-Induced Prolonged Mating in male Drosophila melanogaster DOI Creative Commons
Yanying Sun, Xiaoli Zhang, Zekun Wu

et al.

G3 Genes Genomes Genetics, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 4, 2024

Abstract Male-male social interactions exert a substantial impact on the transcriptional regulation of genes associated with aggression and mating behavior in male Drosophila melanogaster. Throughout our comprehensive genetic screening aggression-related genes, we identified that majority mutants for these are rival-induced visually-oriented behavior, longer-mating-duration (LMD). The upregulated single-housed males significantly altered LMD but not copulation latency, suggesting primary duration. Single-cell RNA sequencing revealed LMD-related predominantly co-expressed male-specific like dsx Cyp6a20 specific cell populations, especially cone cells. Functional validation confirmed roles mediating LMD. Expression Cyp6a20, Cyp4d21, CrzR was enriched cells, disruptions cell-specific expression Cyp4d21 leading to disrupted We also novel gene, CG10026/Macewindu, reversed when overexpressed These findings underscore critical role cells as pivotal site involved behavior. This study provides valuable insights into intricate mechanisms underlying complex sexual behaviors Drosophila.

Language: Английский

Citations

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