Structure and function of the soil microbiome underlying N2O emissions from global wetlands

Nature Communications, Journal Year: 2022, Volume and Issue: 13(1)

Published: March 17, 2022

Abstract Wetland soils are the greatest source of nitrous oxide (N 2 O), a critical greenhouse gas and ozone depleter released by microbes. Yet, microbial players processes underlying N O emissions from wetland poorly understood. Using in situ measurements determining structure potential functional communities 645 soil samples globally, we examined role archaea, bacteria, fungi nitrogen (N) cycling emissions. We show that higher drained warm soils, correlated with diversity further provide evidence despite their much lower abundance compared to nitrifying archaeal is key factor explaining globally. Our data suggest ongoing global warming intensifying environmental change may boost nitrifiers, collectively transforming greater O.

Language: Английский

---

Quantifying nitrous oxide production rates from nitrification and denitrification under various moisture conditions in agricultural soils: Laboratory study and literature synthesis

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 13

Published: Jan. 12, 2023

Biogenic nitrous oxide (N2O) from nitrification and denitrification in agricultural soils is a major source of N2O the atmosphere, its flux changes significantly with soil moisture condition. However, quantitative relationship between production different pathways (i.e., vs. denitrification) content remains elusive, limiting our ability predicting future emissions under changing environment. This study quantified rates various conditions using laboratory incubation combined literature synthesis. 15N labeling approach was used to differentiate eight contents ranging 40 120% water-filled pore space (WFPS) study, while 80 groups data 17 studies across global were collected Results showed that as increased, first increased then decreased, peak occurring 95% WFPS. By contrast, dominant pathway switched 60 70% Furthermore, synthetic elucidated driver controlling relative contributions production, NH4 + NO3 - concentrations mainly determined each pathway. The treatments broad narrow gradient required capture comprehensive response rate change, essential for accurately emission climate change scenarios.

Language: Английский

---

Nitrogen, manganese, iron, and carbon resource acquisition are potential functions of the wild rice Oryza rufipogon core rhizomicrobiome

Microbiome, Journal Year: 2022, Volume and Issue: 10(1)

Published: Nov. 22, 2022

Abstract Background The assembly of the rhizomicrobiome, i.e., microbiome in soil adhering to root, is influenced by conditions. Here, we investigated core rhizomicrobiome a wild plant species transplanted an identical type with small differences chemical factors and impact these chemistry on after long-term cultivation. We sampled three natural reserve populations rice (i.e., situ) situ grown ex for more than 40 years determine rhizomicrobiome. Results Generalized joint attribute modeling (GJAM) identified total 44 amplicon sequence variants (ASVs) composing including 35 bacterial ASVs belonging phyla Actinobacteria, Chloroflexi, Firmicutes, Nitrospirae 9 fungal Ascomycota, Basidiomycota, Rozellomycota. Nine genera Haliangium , Anaeromyxobacter Bradyrhizobium Bacillus were abundant rhizosphere rice. main ecological functions nitrogen fixation, manganese oxidation, aerobic chemoheterotrophy, iron respiration, suggesting roles improving nutrient resource acquisition growth. function community was significantly ( p < 0.05) shaped electrical conductivity, nitrogen, available phosphorus present roots. Conclusion discovered that manganese, iron, carbon are potential Oryza rufipogon . Our findings suggest further utilization should consider effects properties abundances different genera.

Language: Английский

---

Salinity and high pH reduce denitrification rates by inhibiting denitrifying gene abundance in a saline-alkali soil

Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)

Published: Feb. 7, 2023

Abstract Denitrification, as the main nitrogen (N) removal process in farmland drainage ditches coastal areas, is significantly affected by saline-alkali conditions. To elucidate effects of conditions on denitrification, incubation experiments with five salt and salt-alkali gradients three addition levels were conducted a soil followed determination denitrification rates associated functional genes (i.e., nirK / nirS nosZ Clade I) via N 2 /Ar technique combination q PCR. The results showed that decreased 23.83–50.08%, 20.64–57.31% 6.12–54.61% gradient increasing from 1 to 3‰, 8‰, 15‰ under 0.05‰, 0.10‰ 0.15‰ urea conditions, respectively. Similarly, 44.57–63.24% an increase 0.5 8‰. abundance sharply saline condition, while high level . In addition, concentration attenuated reduction , gene abundance. Partial least squares regression (PLSR) models demonstrated salinity, dissolved oxygen (DO) overlying water, concentration, denitrifying key determinants rate environment, pH was additional determinant environment. Taken together, our suggest salinity inhibiting nirS, whereas could alleviate this effect. Our study provides helpful information better understanding reactive fertilizer application areas.

Language: Английский

---

Domesticated rice alters the rhizosphere microbiome, reducing nitrogen fixation and increasing nitrous oxide emissions

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: Feb. 27, 2025

Crop domestication has revolutionized food production but increased agriculture's reliance on fertilizers and pesticides. We investigate differences in the rhizosphere microbiome functions of wild domesticated rice, focusing nitrogen (N) cycling genes. Shotgun metagenomics real-time PCR reveal a higher abundance N-fixing genes rice microbiomes. Validation through transplanting suspensions shows highest nitrogenase activity soils with suspensions, regardless planted type. Domesticated however, exhibits an number associated nitrous oxide (N2O) production. Measurements N2O emissions are significantly soil compared to rice. Comparative root metabolomics between further show that exudates positively correlate frequency microbial genes, as indicated by metagenomic qPCR, respectively. To confirm, we add metabolites black soil, qPCR maximize gene abundances activity. Collectively, these findings suggest negatively impacts bacteria enriches produce greenhouse gas N2O, highlighting environmental trade-offs crop domestication. Domestication crops boosted dependence This study harbors nitrogen-fixing rhizosphere, while more

Language: Английский

---

Contribution of Ammonium-Induced Nitrifier Denitrification to N₂O in Paddy Fields

Environmental Science & Technology, Journal Year: 2023, Volume and Issue: 57(7), P. 2970 - 2980

Published: Jan. 31, 2023

Paddy fields are one of the most important sources nitrous oxide (N2O), but biogeochemical N2O production mechanisms in soil profile remain unclear. Our study used incubation, dual-isotope (15N-18O) labeling methods, and molecular techniques to elucidate characteristics (0-60 cm) during summer fallow, rice cropping, winter fallow periods. The results pointed out that biotic processes dominated (72.2-100%) from tillage layer accounted for 91.0-98.5% total profile. Heterotrophic denitrification (HD) was main process generating N2O, contributing between 53.4 96.6%, remainder being due ammonia oxidation pathways, which further confirmed by metagenomics quantitative polymerase chain reaction (qPCR) assays. Nitrifier (ND) an source, 0-46.6% production, showed similar trends with emissions. Among physicochemical biological factors, ammonium content ratio organic matter nitrate were driving factors affecting contribution ratios pathways HD pathway, respectively. Moisture pH affect norC-carrying Spirochetes thus rate. These findings confirm importance ND help impact anthropogenic activities, including tillage, fertilization, irrigation, on production.

Language: Английский

---

Response characteristics of denitrifying bacteria and denitrifying functional genes to woody peat during pig manure composting

Bioresource Technology, Journal Year: 2023, Volume and Issue: 374, P. 128801 - 128801

Published: Feb. 25, 2023

Language: Английский

---

Microbial and isotopomer analysis of N2O production pathways in a calcareous film-mulched farmland

Biology and Fertility of Soils, Journal Year: 2023, Volume and Issue: 59(4), P. 407 - 422

Published: March 18, 2023

Language: Английский

---

Microbial separator allied biocathode supports simultaneous nitrification and denitrification for nitrogen removal in microbial electrochemical system

Bioresource Technology, Journal Year: 2021, Volume and Issue: 345, P. 126537 - 126537

Published: Dec. 9, 2021

Language: Английский

---

Biochar derived from spent mushroom substrate reduced N2O emissions with lower water content but increased CH4 emissions under flooded condition from fertilized soils in Camellia oleifera plantations

Chemosphere, Journal Year: 2021, Volume and Issue: 287, P. 132110 - 132110

Published: Aug. 31, 2021

Language: Английский

---