Migration of heavy metals and microbial metabolic regulation mechanisms in the co-fermentation of coal slime and sawdust DOI

Yongxin Guan,

Hongyu Guo, Daping Xia

et al.

Environmental Technology, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 15

Published: March 27, 2025

Heavy metals (HMs) significantly affect the anaerobic fermentation of coal slime (CS), while sawdust serves as a promising substrate for methane bioconversion and an effective adsorbent HMs. To explore migration HMs improve conversion efficiency CS to biomethane, experiments were conducted on co-fermentation with different mass ratios. FT-IR, ICP-MS, SEM-EDS, metagenomic sequencing employed elucidate regulation mechanism microorganisms after adding in altering toxicological environment. The results revealed that optimum ratio was 2:1. Oxygen-containing functional groups removed, fatty structure degraded. Addition promoted further degradation various Metal elements such Fe, Mg, Co, Cd had also been detected its surface. Bacillota (39.05%) enriched, which effectively improved microbial community structure. abundance species metal detoxification functions increased. types transporters related toxic ions increased families ATP-binding Cassette Superfamily (ABC) Resistance-Nodulation-Cell Division (RND), would aid cellular homeostasis. Moreover, addition expression resistance genes (MRGs). Multi-MRGs primarily linked Cu, As, Zn, bacterial contributing key MRGs copA, ziaA, actP. These findings offer valuable insights support clean efficient utilization sawdust.

Language: Английский

Migration of heavy metals and microbial metabolic regulation mechanisms in the co-fermentation of coal slime and sawdust DOI

Yongxin Guan,

Hongyu Guo, Daping Xia

et al.

Environmental Technology, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 15

Published: March 27, 2025

Heavy metals (HMs) significantly affect the anaerobic fermentation of coal slime (CS), while sawdust serves as a promising substrate for methane bioconversion and an effective adsorbent HMs. To explore migration HMs improve conversion efficiency CS to biomethane, experiments were conducted on co-fermentation with different mass ratios. FT-IR, ICP-MS, SEM-EDS, metagenomic sequencing employed elucidate regulation mechanism microorganisms after adding in altering toxicological environment. The results revealed that optimum ratio was 2:1. Oxygen-containing functional groups removed, fatty structure degraded. Addition promoted further degradation various Metal elements such Fe, Mg, Co, Cd had also been detected its surface. Bacillota (39.05%) enriched, which effectively improved microbial community structure. abundance species metal detoxification functions increased. types transporters related toxic ions increased families ATP-binding Cassette Superfamily (ABC) Resistance-Nodulation-Cell Division (RND), would aid cellular homeostasis. Moreover, addition expression resistance genes (MRGs). Multi-MRGs primarily linked Cu, As, Zn, bacterial contributing key MRGs copA, ziaA, actP. These findings offer valuable insights support clean efficient utilization sawdust.

Language: Английский

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