Vaginal microbiota changes of persistent human papillomavirus infection after cervical conization DOI Creative Commons

Lingyun Liang,

Cailing Ma, Yan Li

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2025, Volume and Issue: 15

Published: April 14, 2025

Objective We investigated the changes in vaginal microbiota among females with persistent human papillomavirus (HPV) infection following cervical conization Xinjiang, China. Methods A total of 108 female participants were enrolled study, including 37 HPV-positive without (Group P1), after P2), and 34 HPV-negative N). DNA was extracted from secretions, V3-V4 regions bacterial 16S rDNA amplified sequenced using NovaSeq technology. The diversity analysis conducted QIIME2 R software, while phenotypic performed Bugbase software. Results Lactobacillus predominant genus women HPV Xinjiang. Following partial resection, α-diversity decreased, particularly patients who had cleared HPV. Bacterial vaginosis-associated anaerobes common environment, their relative abundance increasing cases infection. Postoperative found to be correlated not only pathogens linked vaginosis but also those associated aerobic vaginitis. Gardnerella Atopobium, as well Bifidobacterium Streptococcus, demonstrated a symbiotic synergy. Both exhibited negative correlations many pathogenic bacteria. Anaerobic biofilm formation most evident phenotypes individuals conization. Conclusion is characterized by coexistence dominance increased microbial diversity. bacteria may play significant role persistence post-surgery, flora under an HPV-infected state warrants further study.

Language: Английский

Vaginal microbiota changes of persistent human papillomavirus infection after cervical conization DOI Creative Commons

Lingyun Liang,

Cailing Ma, Yan Li

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2025, Volume and Issue: 15

Published: April 14, 2025

Objective We investigated the changes in vaginal microbiota among females with persistent human papillomavirus (HPV) infection following cervical conization Xinjiang, China. Methods A total of 108 female participants were enrolled study, including 37 HPV-positive without (Group P1), after P2), and 34 HPV-negative N). DNA was extracted from secretions, V3-V4 regions bacterial 16S rDNA amplified sequenced using NovaSeq technology. The diversity analysis conducted QIIME2 R software, while phenotypic performed Bugbase software. Results Lactobacillus predominant genus women HPV Xinjiang. Following partial resection, α-diversity decreased, particularly patients who had cleared HPV. Bacterial vaginosis-associated anaerobes common environment, their relative abundance increasing cases infection. Postoperative found to be correlated not only pathogens linked vaginosis but also those associated aerobic vaginitis. Gardnerella Atopobium, as well Bifidobacterium Streptococcus, demonstrated a symbiotic synergy. Both exhibited negative correlations many pathogenic bacteria. Anaerobic biofilm formation most evident phenotypes individuals conization. Conclusion is characterized by coexistence dominance increased microbial diversity. bacteria may play significant role persistence post-surgery, flora under an HPV-infected state warrants further study.

Language: Английский

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