Frontiers in Microbiology,
Journal Year:
2024,
Volume and Issue:
15
Published: July 31, 2024
A
cruise
was
conducted
in
the
summer
of
2023
from
Pearl
River
Estuary
(PRE)
to
adjacent
waters
Xisha
Islands
northern
South
China
Sea
(NSCS)
investigate
distribution,
community
structure,
and
assembly
patterns
eukaryotic
prokaryotic
phytoplankton
using
high-throughput
sequencing
(HTS)
microscopic
observation.
Dinophyta
were
most
abundant
phylum
based
on
HTS,
accounting
for
92.17%
total
amplicon
sequence
variants
(ASVs).
Syndiniales
order
among
phytoplankton,
whereas
Prochlorococcus
genus
within
cyanobacteria.
The
alpha
diversity
showed
lowest
values
PRE
area
decreased
gradually
with
depth,
while
cyanobacteria
exhibited
higher
indices
at
depths
ranging
75
m
750
m.
morphological
results
different
data
HTS.
Diatoms
(37
species)
dominated
community,
an
average
abundance
3.01
×
10
4
cells
L
−1
,
but
only
six
species
dinoflagellate
observed.
Spearman
correlation
analysis
redundancy
(RDA)
that
distribution
structure
largely
influenced
by
geographical
location
environmental
parameters
NSCS.
neutral
model
(NCM)
null
indicated
deterministic
processes
played
a
significant
role
heterogeneous
selection
homogeneous
47.27
29.95%,
respectively.
However,
stochastic
(over
60%)
undominated
accounted
63.44%.
In
summary,
formation
mainly
factors
geographic
location,
shaped
both
processes,
which
over
60%,
Ecology and Evolution,
Journal Year:
2023,
Volume and Issue:
13(5)
Published: May 1, 2023
Harmful
algal
blooms
(HABs)
have
occurred
more
frequently
in
recent
years.
In
this
study,
to
investigate
their
potential
impact
the
Beibu
Gulf,
short-read
and
long-read
metabarcoding
analyses
were
combined
for
annual
marine
phytoplankton
community
HAB
species
identification.
Short-read
showed
a
high
level
of
biodiversity
area,
with
Dinophyceae
dominating,
especially
Gymnodiniales.
Multiple
small
phytoplankton,
including
Prymnesiophyceae
Prasinophyceae,
also
identified,
which
complements
previous
lack
identifying
those
unstable
after
fixation.
Of
top
20
genera
15
HAB-forming
genera,
accounted
47.3%-71.5%
relative
abundance
phytoplankton.
Based
on
metabarcoding,
total
147
OTUs
(PID
>
97%)
belonging
identified
at
level,
118
species.
Among
them,
37
belonged
species,
98
reported
first
time
Gulf.
Contrasting
two
approaches
class
they
both
predominance
Dinophyceae,
included
abundances
Bacillariophyceae,
Prymnesiophyceae,
but
contents
classes
varied.
Notably,
results
quite
different
below
genus
level.
The
diversity
probably
due
special
life
history
multiple
nutritional
modes.
Annual
variation
revealed
study
provided
basis
evaluating
aquaculture
even
nuclear
power
plant
safety
Marine Environmental Research,
Journal Year:
2023,
Volume and Issue:
191, P. 106175 - 106175
Published: Sept. 12, 2023
Phytoplankton
monitoring
is
essential
for
the
global
understanding
of
aquatic
ecosystems.
The
present
research
studies
phytoplankton
community
Urdaibai
estuary,
combining
microscopy
and
eDNA
metabarcoding
first
time
in
area.
main
aims
were
to
describe
composition
relation
environmental
conditions
compare
two
methods
used.
Diatoms
Minutocellus
polymorphus
Chaetoceros
tenuissimus
dominated
outer
being
replaced
by
Teleaulax
acuta
(cryptophyte),
Kryptoperidinium
foliaceum
(dinoflagellate)
Cyclotella
spp.
(diatom)
towards
inner
This
change
was
mainly
prompted
salinity
nutrients.
Metabarcoding
revealed
presence
223
species
that
not
observed
previous
estuary.
However,
several
characteristic
(e.g.,
K.
foliaceum)
only
detected
with
microscopy.
Additionally,
covered
limitations
concerning
quantification.
Thus,
give
a
full
insight,
combination
techniques
recommended.
Scientific Reports,
Journal Year:
2024,
Volume and Issue:
14(1)
Published: March 18, 2024
Two
gene
regions
commonly
used
to
characterise
the
diversity
of
eukaryotic
communities
using
metabarcoding
are
18S
ribosomal
DNA
V4
and
V9
regions.
We
assessed
effectiveness
these
two
for
characterising
diverisity
coastal
microalgae
(EMCs)
from
tropical
temperate
sites.
binned
amplicon
sequence
variants
(ASVs)
into
high
level
taxonomic
groups:
dinoflagellates,
pennate
diatoms,
radial
centric
polar
chlorophytes,
haptophytes
'other
microalgae'.
When
generated
ASV
abundances
were
compared,
region
a
higher
number
raw
reads,
captured
more
all
groups
was
closely
aligned
with
community
composition
determined
light
microscopy.
The
did
resolve
ASVs
deeper
resolution
within
but
not
effectively
other
major
divisions.
via
metabarcoding,
use
multiple
is
recommended,
can
be
in
isolation
provide
high-level
biodiversity
reflect
relative
groups.
This
approach
reduces
cost
sequencing
whilst
still
providing
important
baseline
ecosystem
function
information.
The ISME Journal,
Journal Year:
2024,
Volume and Issue:
18(1)
Published: Jan. 1, 2024
Abstract
Chirality,
a
fundamental
property
of
matter,
is
often
overlooked
in
the
studies
marine
organic
matter
cycles.
Dihydroxypropanesulfonate
(DHPS),
globally
abundant
organosulfur
compound,
serves
as
an
ecologically
important
currency
for
nutrient
and
energy
transfer
from
phytoplankton
to
bacteria
ocean.
However,
chirality
DHPS
nature
its
transformation
remain
unclear.
Here,
we
developed
novel
approach
using
chiral
phosphorus-reagent
labeling
separate
enantiomers.
Our
findings
demonstrated
that
at
least
one
enantiomer
present
diatoms
coccolithophores,
both
enantiomers
are
widespread
environments.
A
chiral-selective
catabolic
pathway
was
identified
Roseobacteraceae
strains,
where
HpsO
HpsP
dehydrogenases
gateway
catabolism
act
specifically
on
R-DHPS
S-DHPS,
respectively.
also
substrate
dehydrogenase
HpsN.
All
three
generate
stable
hydrogen
bonds
between
chirality-center
hydroxyls
highly
conserved
residues,
form
coordinate–covalent
Zn2+,
which
determines
mechanistic
basis
strict
stereoselectivity.
We
further
illustrated
role
enzymatic
promiscuity
evolution
metabolism
SAR11.
This
study
provides
first
evidence
chirality’s
involvement
phytoplankton-bacteria
metabolic
currencies,
opening
new
avenue
understanding
ocean
cycle.
Ecotoxicology and Environmental Safety,
Journal Year:
2025,
Volume and Issue:
291, P. 117834 - 117834
Published: Feb. 1, 2025
The
diversity
and
spatial-temporal
distribution
of
phytoplankton
HAB
species,
contamination
status
oyster
toxin,
their
sources
were
investigated
in
Jiangmen
farming
area
based
on
morphological
observation
liquid
chromatography-tandem
mass
spectrometry
analysis.
results
revealed
there
28
including
19
harmless
two
toxic
species
(Akashiwo
sanguinea
Karenia
brevis),
seven
toxin-producing
microalgae
(Alexandrium
pacificum,
Dinophysis
caudata,
D.
miles,
fortii,
Gonyaulax
spinifera,
Gymnodinium
catenatum,
K.
mikimotoi).
mean
abundance
total
generally
showed
a
trend
increasing
from
winter
to
autumn.
average
abundances
269,
265,
321
2.6
×
103
cells
L-1
winter,
spring,
summer
autumn,
respectively.
Redundancy
analysis
temperature,
dissolved
oxygen,
silicate
phosphate
the
key
factors
related
with
variations
species.
Only
spring
samples
detected
paralytic
shellfish
toxins
(PSTs)
(1/15),
composition
included
gonyautoxins
(GTX1&2)
decarbamoyl
gonyautoxin
2
(dcGTX2),
toxicity
level
9.96
µg
STXeq
kg-1.
N-sulfocarbamyl
(C1)
3
(dcGTX3)
observed
net-concentrated
samples.
It
is
inferred
that
these
five
types
PSTs
derived
A.
pacificum.
cultured
oysters
low-concentration
lipophilic
marine
(LMTs)
okadaic
acid
(OA),
dinophysis-1
(DTX1),
pectenotoxin-2
(PTX2),
gymnodimine
(GYM),
homo-yessotoxin
(homo-YTX).
Though
levels
LMTs
low,
presence
highlights
potential
threat
safety
products
Research Square (Research Square),
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 27, 2025
Abstract
As
a
state
with
high
shellfish
aquaculture
production
in
the
US,
it
is
essential
that
New
Jersey
(NJ)
managers
identify
phycotoxins
of
concern
and
their
spatiotemporal
distribution.
However,
to
date,
there
are
no
publications
on
phycotoxin
presence
or
concentrations
NJ
estuaries.
Passive
samplers
valuable
for
assessing
emerging
threats
long-term
trends
phycotoxins,
but
levels
phenolic
compounds
from
Pinelands
may
interfere
by
competing
binding
sites
resin.
The
objectives
this
study
were
(1)
establish
baseline
two
estuaries
using
passive
samplers,
(2)
determine
impact
content
waters
samplers.
Seven
detected:
azaspiracids-1
(AZA1),
azaspiracids-2
(AZA2),
okadaic
acid,
dinophysistoxin-1,
pectenotoxin-2,
yessotoxin
(YTX),
goniodomin
A
(GDA).
This
first
publication
YTX
US
East
Coast
farthest
north
GDA,
AZA1
AZA2
have
been
quantified
Coast.
No
microcystins
detected,
adsorption/desorption
resin
strongly
impacted
during
controlled
experiment.
complexity
coastal
water
also
analysis
all
other
as
depicted
elevated
suppression
compared
methanolic
standards.
Phytoxigene™
revealed
cyanobacteria
phycotoxin-producing
genes
cylindrospermopsin
saxitoxin.
Results
indicated
need
extended
annual
monitoring
NJ,
further
research
causative
species
these
caution
when
particular
environmental
conditions,
efficacy
be
compromised.
