First report of toxigenic Amphidoma languida (Amphidomataceae, Dinophyceae) from the Pacific, with reference to intracellular ultrastructure and azaspiracid compounds DOI Creative Commons
Koyo Kuwata, David U. Hernández‐Becerril, Mayu Ozawa

et al.

Phycological Research, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 29, 2024

SUMMARY Morphology, phylogeny and azaspiracid (AZA) production of a strain Amphidoma languida isolated from the subtropical Mexican Pacific were examined. Scanning electron microscopy showed thecal plate arrangement Am . Mexico as Po, cp, X, 6′, 6′′, 6C, 5S, 6′′′, 2′′′′, with prominent apical pore complex, ventral on anterior 1′ near suture to 6′ plate, antapical 2′′′′ which are congruent those reported Atlantic. The depression was occasionally observed end Sa plate. Cells had dinokaryotic nucleus positioned in posterior part cell parietal yellowish chloroplasts spherical pyrenoid episome. Transmission crystalline vesicles tubular cytoplasmic invaginations pyrenoid, resembled most related non‐toxigenic species fulgens intracellular components, different , oil droplets lipid‐associated membranous structures. AZA‐2 detected by liquid chromatography‐tandem mass spectrometry quota 30.9 fg −1 Three other AZA‐related compounds also detected. This is first report AZA‐producing Ocean.

Language: Английский

The combination of microscopic and metabarcoding analyses revealed a high diversity of algal bloom species in the Taiwan Strait DOI
Yuning Zhang,

Lanping Lin,

Zheng Hu

et al.

Marine Pollution Bulletin, Journal Year: 2025, Volume and Issue: 216, P. 118027 - 118027

Published: April 22, 2025

Language: Английский

Citations

0
Optimization and evaluation of 18S rDNA V4-based metabarcoding analysis of Tripos species DOI
Yingchao Li, Nansheng Chen

Journal of Applied Phycology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 4, 2025

Language: Английский

Citations

0
Phylogeny and ultrastructure of a non-toxigenic dinoflagellate Amphidoma fulgens sp. nov. (Amphidomataceae, Dinophyceae), with a wide distribution across Asian Pacific DOI Creative Commons
Koyo Kuwata, Wai Mun Lum, Kazuya Takahashi

et al.

Harmful Algae, Journal Year: 2024, Volume and Issue: 138, P. 102701 - 102701

Published: July 26, 2024

Amphidoma languida, a marine thecate dinoflagellate that produces the lipophilic toxin azaspiracids (AZAs), is primarily found in Atlantic. Although this species has not been recorded Asian Pacific, environmental DNAs related to Am. languida have widely detected region by metabarcoding analysis. Their morphology and AZA production remain unclear. In study, morphology, ultrastructure, phylogeny, of nine strains isolated from Japan, Malaysia, Philippines were investigated. Phylogenetic trees inferred rDNAs (SSU, ITS, LSU rDNA) showed monophyly Pacific sister clade, including toxigenic Cells ellipsoid, 8.7-16.7 µm length 7.4-14.0 width, with conspicuous apical pore complex. A large nucleus hyposome, parietal chloroplast spherical pyrenoid episome, refractile bodies observed. Thecal tabulation was typical Amphidoma, Po, cp, X, 6', 6'', 6C, 5S, 6''', 2''''. ventral located on anterior 1' plate, beside suture 6' plate. The presence depression, sulcal different languida. antapical pore, containing approximately 10 small pores, apparently smaller than trioculata, possessing three pores (ventral pore). TEM crystalline structures, resembling guanine crystals, cytoplasmic invaginations into matrix. Flagellar apparatus lacking striated root connective similar peridinioids dinoflagellates. AZAs LC-MS/MS. This non-toxigenic species, here we propose as fulgens sp. nov., distributed Pacific. Moreover, molecular comparison also suggested most DNA sequences previously reported or attributable fulgens.

Language: Английский

Citations

3
A new dinoflagellate Gonyaulax pospelovana with resting cysts resembling Spiniferites delicatus and its biogeography and ecology revealed by DNA metabarcoding DOI
Haifeng Gu, Jing Zheng,

Shuning Huang

et al.

Phycologia, Journal Year: 2023, Volume and Issue: 63(1), P. 74 - 88

Published: Dec. 21, 2023

Extant species of the dinoflagellate genus Gonyaulax are capable producing resting cysts morphologically similar to different cyst-based genera, and their cyst-theca relationships far from resolved. Here we have carried out germination experiments on several living that resemble Spiniferites delicatus subtropical regions China Mexico. Both cyst theca morphology were examined by light scanning electron microscopy. A new species, pospelovana, is described, characterized a cingulum displacement an overhang twice its width, two short antapical spines. The G. pospelovana had granular surface gonal processes with petaloid tips. Maximum likelihood Bayesian inference analyses based LSU SSU rRNA gene sequences revealed strains identified as monophyletic, forming sister clade ellegaardiae presumable spinifera. One Chinese strain Mexican for yessotoxin production using LC-MS/MS, but not found produce detectable amount toxins. Metabarcoding targeting 18S V4 was performed monthly collected samples in Xiamen Bay, China. ZOTU (zero-radius operational taxonomic units) detected consistent pospelovana. Its maximum abundance recorded summer. Additionally, OTU Tara Oceans metabarcoding data, which occurred Indian Pacific oceans at temperatures ranging 28°C 31°C, suggesting it warm water species.

Language: Английский

Citations

4
A new small thecate dinoflagellate Azadinium anteroporum sp. nov. (Amphidomataceae, Dinophyceae) isolated from the Asian Pacific DOI
Koyo Kuwata, Wai Mun Lum, Kazuya Takahashi

et al.

Phycologia, Journal Year: 2023, Volume and Issue: 62(4), P. 303 - 314

Published: May 29, 2023

The marine thecate dinoflagellate Azadinium includes species known to produce the diarrhetic shellfish toxins as azaspiracids (AZAs). In this study, morphology of a isolated from Mutsu Bay, Japan, was examined by LM and SEM, its phylogenetic position inferred ITS LSU rDNA sequences. production AZAs liquid chromatography-triple quadrupole mass spectrometer (LC-MS/MS). Cells were elliptical, 15.9–22.4 µm long 11.1–17.1 wide. A chloroplast positioned peripherally, with pyrenoid near cingulum, nucleus placed in hyposome. hyposome smaller than episome, an antapical spine on plate 2''''. Thecal tabulation Po, cp, X, 4', 3a, 6'', 6C, 5S, 6''', apical pore complex consisted symmetrical (Po), cover (cp) small canal (X), surrounded prominent collar formed plates. ventral located mid-anterior edge 1' contacted X-plate and/or left Po. Plate 1'' contact 1a. AZA not detected cultures. Molecular phylogeny showed that related possess side 1', i.e. A. cuneatum, dalianense, obesum, poporum, spinosum trinitatum, but it included these species. new anteroporum sp. nov. differs (mid-anterior 1') (which have 1'), polongum (with posterior all other right 1').

Language: Английский

Citations

2
First report of toxigenic Amphidoma languida (Amphidomataceae, Dinophyceae) from the Pacific, with reference to intracellular ultrastructure and azaspiracid compounds DOI Creative Commons
Koyo Kuwata, David U. Hernández‐Becerril, Mayu Ozawa

et al.

Phycological Research, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 29, 2024

SUMMARY Morphology, phylogeny and azaspiracid (AZA) production of a strain Amphidoma languida isolated from the subtropical Mexican Pacific were examined. Scanning electron microscopy showed thecal plate arrangement Am . Mexico as Po, cp, X, 6′, 6′′, 6C, 5S, 6′′′, 2′′′′, with prominent apical pore complex, ventral on anterior 1′ near suture to 6′ plate, antapical 2′′′′ which are congruent those reported Atlantic. The depression was occasionally observed end Sa plate. Cells had dinokaryotic nucleus positioned in posterior part cell parietal yellowish chloroplasts spherical pyrenoid episome. Transmission crystalline vesicles tubular cytoplasmic invaginations pyrenoid, resembled most related non‐toxigenic species fulgens intracellular components, different , oil droplets lipid‐associated membranous structures. AZA‐2 detected by liquid chromatography‐tandem mass spectrometry quota 30.9 fg −1 Three other AZA‐related compounds also detected. This is first report AZA‐producing Ocean.

Language: Английский

Citations

0