A Comparative Study of the In Vitro Intestinal Permeability of Pinnatoxins and Portimine

Marine Drugs, Journal Year: 2025, Volume and Issue: 23(1), P. 26 - 26

Published: Jan. 7, 2025

The pinnatoxins (PnTXs) and portimines, produced by Vulcanodinium rugosum, have been detected in several countries, raising concerns for human health. Although no poisoning from these toxins has reported so far, they shown to distribute throughout the rodent body after oral administration. Therefore, we investigated impact of PnTX analogs (PnTX-A, -E, -F, -G, -H) portimine (8, 16, 32 ng/mL) on intestinal barrier integrity their bioavailability using Caco-2 cell monolayers treated 2, 6, 24 h. Our results demonstrated that all could impair h, with differences observed PnTX-A, as well portimine, most potent all. While PnTX-A -E exhibited poor permeability, other PnTXs were more penetrative, a Papp > 1.5 × 10-6 cm·s-1. Portimine was only toxin displaying both time- concentration-dependent passage, likely involving passive diffusion process. experimental compared predictions obtained QSAR tools. qualitative, our suggest some compounds may be distributed body. Further vivo studies are required estimate potential public health concerns.

Language: Английский

Detection of the Cyclic Imines Pinnatoxin G, 13-Desmethyl Spirolide C and 20-Methyl Spirolide G in Bivalve Molluscs from Great Britian

Marine Drugs, Journal Year: 2024, Volume and Issue: 22(12), P. 556 - 556

Published: Dec. 12, 2024

Harmful algal biotoxins in the marine environment are a threat to human food safety due their bioaccumulation bivalve shellfish. Whilst official control monitoring provides ongoing risk management for regulated toxins live molluscs, no routine system is currently operation UK other non-regulated toxins. To assess potential presence of such compounds, systematic screen shellfish was conducted throughout Great Britain. A rapid dispersive methanolic extraction used with UHPLC-MS/MS analysis test fifteen cyclic imines and seven brevetoxins 2671 samples taken from designated harvesting areas around Britain during 2018. Out 22 incorporated into method, only pinnatoxin G, 13-desmethyl spirolide C 20-methyl G were detected, maximum concentrations 85.4 µg/kg, 13.4 µg/kg 51.4 respectively. follow up study G-positive examined its esterification fatty acids concluded that following hydrolysis, concentration increased by an average 8.6%, tentative identification these esters determined LC-HRMS. This highlights requirement emerging threats toxicological assessment studies.

Language: Английский