Improvement of Technologies for Isolation and Purification of Biologically Active Substances from Plant Raw Materials DOI Creative Commons
Г. Н. Турманидзе, В. В. Сорокин, K. S. Stepanov

et al.

Drug development & registration, Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 12, 2023

Introduction. Improving the technologies for isolating and purifying biologically active substances from plant materials is an important task pharmaceutical, food cosmetic industries. Technology development often requires changes to design of existing equipment. During modernization equipment implementation new technologies, it possible improve its configuration, which makes significantly increase yield at minimal cost. To efficiency process hesperidin materials, we proposed use a number technological solutions. In particular, mixing devices with configuration impellers obtained by computational fluid dynamics, when using Soxhlet extractor heated extraction chamber, adding external coil jacket, regulate temperature inside chamber accelerate processes diffusion mass transfer, select solvent extractant on basis thermodynamic models calculation. Aim. technology modernized Materials methods. Flow distribution in apparatuses was modeled dynamics evaluate results modeling, experiments were carried out, object peel orange (dried flavedo albedo, grinding degree 0.1–0.2 mm, moisture content 3.5 %). The quantitative flavonoid complex terms determined direct spectrophotometry wavelength 290 nm, gravimetrically. Statistical data processing performed Minitab v21 software (Minitab Inc., USA), differences considered statistically significant p < 0.05. Results discussion. Based methods intensify dissolution stages preliminary degreasing raw extraction, six-bladed impeller designed, these creating axial radial flows movement capacitive apparatus maintaining suspended state volume liquid low speeds energy consumption. Equipping coiled jacket made solubility substance extract more target component one cycle. A isolation purification developed, parameters optimized. Conclusion. As result study, shown that selecting method dynamics. isolate poorly soluble compounds dense (roots, bark, etc.), modification proposed. Modernization typical units obtain up 95 % purity 90 single recrystallization.

Language: Английский

Bioactivity screening and molecular identification of Anchusa milleri L. sunflower crud extract for antioxidant, antiviral, antimicrobial, and anticancer properties DOI
Hammad Khalifeh Aldal’in, Nada K. Alharbi, Ameer M. Hadi

et al.

Natural Product Research, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 14

Published: Aug. 24, 2024

The current study evaluates hot water A. milleri sunflower extracts at 40 °C temperature (AMSE40) for their antibacterial, anti-biofilm, antiviral and anticancer activities. AMSE40 exhibited excellent antioxidant activity with an IC50 of 1.17 mg/mL (ascorbic acid) was found to be rich in phytochemical compounds such as alkaloids, flavonoids, phenolics, saponins, tannins, concentrations 1.23%, 351.60 mg/g, 152.50 0.98%, 146.35 respectively. showed strong antibacterial anti-biofilm against four multidrug-resistant isolates, comprising E. faecalis, S. aureus, P. aeruginosa, K. pneumonia minimum bactericidal (25 mg/mL) inhibitory (12.5 all isolates. (62.5 µg/mL) efficacy CoxB4 (9.1%) HSV-1 (34.4%). Additionally, induced DNA fragmentation liver cell lines, indicating death. cytotoxic concentration had a mild impact on Vero (283.2 µg) HepG2 cells (76.4 µg). has the potential serve natural eco-friendly source innovative pharmaceutical medical applications.

Language: Английский

Citations

0
Exploring phytochemicals and pharmacological properties of Populus × tomentiglandulosa DOI Creative Commons

Hak-Dong Lee,

Ji Hyun Kim,

Jong Hee Choi

et al.

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: Aug. 28, 2024

Populus × tomentiglandulosa (PT), a tree endemic to Korea, shows promising potential as natural therapeutic agent owing its potent anti-inflammatory properties. However, the isolation and analysis of phytochemical compounds in PT related species remains underexplored. Therefore, this study aims investigate biochemical profile evaluate extracts fractions for activities. Nine were isolated, including two novel flavonoids (luteolin 7-O- β - d- glucuronide butyl ester chrysoeriol ester) from Salicaceae family first time. The ethyl acetate fraction exhibited significant radical scavenging activity against various radicals, DPPH, ABTS + , • OH, O 2 – radicals. showed minimal cytotoxicity Raw 264.7 macrophages at concentrations below 500 100 μg/mL, respectively. Furthermore, significantly suppressed protein expression proinflammatory mediators (iNOS IL-6) LPS-stimulated macrophages, highlighting their effects. These findings suggest that holds promise valuable intervention oxidative stress inflammation-related disorders, underscoring need further exploration clinical applications.

Language: Английский

Citations

0
Response of Pelargonium graveolens under variance of geographical distribution DOI Creative Commons
Imen Ben Lahmar,

Lyubov Yotova

JOURNAL OF OASIS AGRICULTURE AND SUSTAINABLE DEVELOPMENT, Journal Year: 2024, Volume and Issue: 6(04), P. 10 - 17

Published: Dec. 26, 2024

The present study evaluated the influence of habitat characteristics on phytochemical composition and antioxidant properties Pelargonium graveolens. Among different sites assessed, Mahdia area showed highest protein fat content at 6.8% 3.7%, respectively. Additionally, plants from same region exhibited energy value 412.1 kcal/100g. originating Kairouan displayed elevated levels Mg Ca, followed by those collected Sejnane region. Notably, iron was observed in harvested Mahdia. These variations were attributed to several factors such as geographical location, altitude average rainfall. Extract demonstrated secondary metabolites, with total phenolic 388 mg GAE/g flavonoid 80 QE/g. Regarding activity, graveolens Kairouan, most potent scavenging activity against DPPH ferric reducing power. results underscored species' potential a valuable natural source bioactive compounds within its ecological niche.

Language: Английский

Citations

0
Viral Infections and Medicinal Plants: A Review of Metabolomics Evidence for the Antiviral Properties and Potentials in Plant Sources DOI Open Access

Bamise Wilson Adeosun,

Du Toit Loots

Published: Dec. 14, 2023

Plants have developed unique mechanisms to cope with the harsh environmental conditions compensate for their lack of mobility. A key part coping is synthesis secondary metabolites. In addition role in plants’ defense against pathogens, they also possess therapeutic properties diseases, and use by humans predates written history. Viruses are a class submicroscopic agents, incapable independent existence outside living host. Pathogenic viruses continue pose significant threat on global health, leading unprecedented fatalities yearly basis. The medicinal plants as natural source an-tiviral agents has been widely reported literature past decades. Metabolomics powerful research tool identification plant metabolites antiviral potentials can be used isolate compounds activities study biosynthetic pathways involved viral disease progression. This review discusses special focus metabolomics evidence supporting efficacy. Additionally, suggestions made optimization various methods characterizing bio-active subsequent understanding mechanism operation.

Language: Английский

Citations

1
Improvement of Technologies for Isolation and Purification of Biologically Active Substances from Plant Raw Materials DOI Creative Commons
Г. Н. Турманидзе, В. В. Сорокин, K. S. Stepanov

et al.

Drug development & registration, Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 12, 2023

Introduction. Improving the technologies for isolating and purifying biologically active substances from plant materials is an important task pharmaceutical, food cosmetic industries. Technology development often requires changes to design of existing equipment. During modernization equipment implementation new technologies, it possible improve its configuration, which makes significantly increase yield at minimal cost. To efficiency process hesperidin materials, we proposed use a number technological solutions. In particular, mixing devices with configuration impellers obtained by computational fluid dynamics, when using Soxhlet extractor heated extraction chamber, adding external coil jacket, regulate temperature inside chamber accelerate processes diffusion mass transfer, select solvent extractant on basis thermodynamic models calculation. Aim. technology modernized Materials methods. Flow distribution in apparatuses was modeled dynamics evaluate results modeling, experiments were carried out, object peel orange (dried flavedo albedo, grinding degree 0.1–0.2 mm, moisture content 3.5 %). The quantitative flavonoid complex terms determined direct spectrophotometry wavelength 290 nm, gravimetrically. Statistical data processing performed Minitab v21 software (Minitab Inc., USA), differences considered statistically significant p < 0.05. Results discussion. Based methods intensify dissolution stages preliminary degreasing raw extraction, six-bladed impeller designed, these creating axial radial flows movement capacitive apparatus maintaining suspended state volume liquid low speeds energy consumption. Equipping coiled jacket made solubility substance extract more target component one cycle. A isolation purification developed, parameters optimized. Conclusion. As result study, shown that selecting method dynamics. isolate poorly soluble compounds dense (roots, bark, etc.), modification proposed. Modernization typical units obtain up 95 % purity 90 single recrystallization.

Language: Английский

Citations

0