Tropical Journal of Natural Product Research,
Journal Year:
2024,
Volume and Issue:
8(1)
Published: Feb. 1, 2024
A
substantial
quantity
of
solid
fish
by-products
is
generated
during
the
processing.
These
offer
an
intriguing
opportunity
as
a
source
high
added-value
compounds,
including
digestive
proteases.
Indeed,
trypsin
one
most
beneficial
and
useful
biomolecules
that
can
be
recovered
from
wastes.
Additionally,
protein
hydrolysates
have
emerged
valuable
abundant
high-quality
bioactive
molecules,
which
efficiently
through
enzymatic
hydrolysis.
This
study
aims
to
provide
insights
into
extraction
utilization
trypsin,
well
derived
by-products,
specifically
those
sardine
(Sardina
pilchardus).
Firstly,
viscera
was
purified
with
approximate
fourteen-fold
increase
in
specific
activity
using
ammonium
sulfate
precipitation,
followed
by
DEAE-cellulose
chromatography.
SDS–PAGE
analysis
revealed
single
band
approximately
27
kDa.
Interestingly,
enzyme
exhibited
significant
features,
optimum
temperature
60
°C,
stability
at
low
temperatures,
retaining
respectively
100%
64%
its
initial
20
°C
50
°C.
The
also
demonstrated
excellent
pH
range
5–10
8.
Furthermore,
it
maintained
40%
11.
inhibited
benzamidine
PMSF
partially
EDTA.
Subsequently,
four
(H1,
H2,
H3,
H4)
were
prepared
sardine,
other
bacterial
All
noteworthy
antioxidant
properties
vitro,
indicating
their
promising
potential
for
application
functional
foods
natural
preservatives.
Journal of Clinical Medicine,
Journal Year:
2023,
Volume and Issue:
12(21), P. 6867 - 6867
Published: Oct. 31, 2023
Nanotechnology
has
emerged
as
a
promising
technology
in
the
field
of
hepatocellular
carcinoma
(HCC),
specifically
implementation
diagnosis
and
treatment
strategies.
Nanotechnology-based
approaches,
such
nanoparticle-based
contrast
agents
nanoscale
imaging
techniques,
have
shown
great
potential
for
enhancing
sensitivity
specificity
HCC
detection.
These
approaches
provide
high-resolution
allow
detection
molecular
markers
alterations
cellular
morphology
associated
with
HCC.
In
terms
treatment,
nanotechnology
revolutionized
therapy
by
enabling
targeted
drug
delivery,
therapeutic
efficacy,
minimizing
off-target
effects.
Nanoparticle-based
carriers
can
be
functionalized
ligands
specific
to
cells,
allowing
selective
accumulation
at
tumor
site.
Furthermore,
facilitate
combination
co-encapsulating
multiple
drugs
within
single
nanoparticle,
synergistic
effects
overcoming
resistance.
This
review
aims
an
overview
recent
advances
nanotechnology-based
Further
research
is
needed
optimize
design
functionality
nanoparticles,
improve
their
biocompatibility
stability,
evaluate
long-term
safety
efficacy.
Nonetheless,
integration
management
holds
promise
may
lead
improved
patient
outcomes
future.
Bioengineering,
Journal Year:
2024,
Volume and Issue:
11(8), P. 817 - 817
Published: Aug. 12, 2024
Personalized
orthopedic
devices
are
increasingly
favored
for
their
potential
to
enhance
long-term
treatment
success.
Despite
significant
advancements
across
various
disciplines,
the
seamless
integration
and
full
automation
of
personalized
treatments
remain
elusive.
This
paper
identifies
key
interdisciplinary
gaps
in
integrating
automating
advanced
technologies
treatment.
It
begins
by
outlining
standard
clinical
practices
extent
personalization
achievable.
The
then
explores
recent
innovations
artificial
intelligence,
biomaterials,
genomic
proteomic
analyses,
lab-on-a-chip,
medical
imaging,
image-based
biomechanical
finite
element
modeling,
biomimicry,
3D
printing
bioprinting,
implantable
sensors,
emphasizing
contributions
treatments.
Tentative
strategies
or
solutions
proposed
address
utilizing
innovative
technologies.
findings
highlight
need
non-invasive
quantitative
assessment
bone
quality,
patient-specific
biocompatibility,
device
designs
that
individual
biological
mechanical
conditions.
comprehensive
review
underscores
transformative
these
importance
multidisciplinary
collaboration
integrate
automate
them
into
a
cohesive,
intelligent
system
Tropical Journal of Natural Product Research,
Journal Year:
2024,
Volume and Issue:
8(1)
Published: Feb. 1, 2024
A
substantial
quantity
of
solid
fish
by-products
is
generated
during
the
processing.
These
offer
an
intriguing
opportunity
as
a
source
high
added-value
compounds,
including
digestive
proteases.
Indeed,
trypsin
one
most
beneficial
and
useful
biomolecules
that
can
be
recovered
from
wastes.
Additionally,
protein
hydrolysates
have
emerged
valuable
abundant
high-quality
bioactive
molecules,
which
efficiently
through
enzymatic
hydrolysis.
This
study
aims
to
provide
insights
into
extraction
utilization
trypsin,
well
derived
by-products,
specifically
those
sardine
(Sardina
pilchardus).
Firstly,
viscera
was
purified
with
approximate
fourteen-fold
increase
in
specific
activity
using
ammonium
sulfate
precipitation,
followed
by
DEAE-cellulose
chromatography.
SDS–PAGE
analysis
revealed
single
band
approximately
27
kDa.
Interestingly,
enzyme
exhibited
significant
features,
optimum
temperature
60
°C,
stability
at
low
temperatures,
retaining
respectively
100%
64%
its
initial
20
°C
50
°C.
The
also
demonstrated
excellent
pH
range
5–10
8.
Furthermore,
it
maintained
40%
11.
inhibited
benzamidine
PMSF
partially
EDTA.
Subsequently,
four
(H1,
H2,
H3,
H4)
were
prepared
sardine,
other
bacterial
All
noteworthy
antioxidant
properties
vitro,
indicating
their
promising
potential
for
application
functional
foods
natural
preservatives.
