Allopatric populations of Drosophila ananassae exhibit different degree of trade-off for evolving immune response against bacterial infection DOI Creative Commons
Pallab Paul, Goutam Mukherjee, Sayarneel Mandal

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 29, 2023

Abstract We conducted laboratory based selection experiment with two allopatric populations of Drosophila ananassae collected from isolated geographic regions India namely, Mysuru and Port Blair subsequently inbred for several generations at laboratory. The were infected enteritically the bacteria Pseudomonas aeruginosa fifty-five followed by ten under relaxed selection. tested development immune-competence selected flies through inquired if elicitation immune responses needed to pay cost trading-off life history morphological traits. observed gradual progressive increase in longevity, reproductive span, post developmental time, fecundity rate, fat content reduction body length water content. Under regime there was a reversion all traits but not basal level. Responses exhibited infected-selected differed, former group more length, content, greater antioxidant enzyme activity, longer infection period, survival than later group. On other side, showed deposition, faster oviposition time flies. Interestingly, exhibited, ovipository period contrast that decrease same. inferred D. show different trade-off evolving response

Language: Английский

Automated survival monitoring system: exploring starvation resistance in newly hatched larvae DOI Creative Commons

Hiroto Ohki,

Ki‐Hyeon Seong, Takahiro Suzuki

et al.

Applied Entomology and Zoology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 11, 2025

Language: Английский

Citations

0
Latitudinal Clines in Climate and Sleep Patterns Shape Disease Outcomes in Drosophila melanogaster Infected by Metarhizium anisopliae DOI Creative Commons

Mintong Nan,

Jonathan B. Wang,

Michail Siokis

et al.

Ecology and Evolution, Journal Year: 2025, Volume and Issue: 15(3)

Published: Feb. 28, 2025

ABSTRACT Major latitudinal clines have been observed in Drosophila melanogaster , a human commensal that originated tropical Africa and has subsequently dispersed globally to colonize temperate habitats. However, despite the crucial role pathogens play species distribution, our understanding of how geographical factors influence disease susceptibility remains limited. This investigation explored effects biomes on resistance using common fly pathogen Metarhizium anisopliae 43 global populations. The findings revealed correlations between gradients sleep duration, temperature, humidity. Although enhanced defenses may be driven by fungal diversity at latitudes, most disease‐resistant males also showed highest desiccation. suggests potential trade‐offs abiotic stress resistance, necessary for survival habitats, resistance. Furthermore, study uncovered interactions sex, mating status, sleep, stresses, affecting Notably, longer‐sleeping virgin flies survived infections longer, with additional daytime post‐infection being protective, particularly resistant lines. These observations support hypothesis defense are intertwined traits linked organismal fitness subject joint clinal evolution.

Language: Английский

Citations

0
Allopatric populations of Drosophila ananassae exhibit different degrees of trade-off for evolving immune response against bacterial infection DOI
Pallab Paul, Goutam Mukherjee, Sayarneel Mandal

et al.

Ethology Ecology & Evolution, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 47

Published: Feb. 4, 2025

Language: Английский

Citations

0
3D chromatin structures associated with ncRNA roX2 for hyperactivation and coactivation across the entire X chromosome DOI Creative Commons
Simon Zhongyuan Tian, Yang Yang, Duo Ning

et al.

Science Advances, Journal Year: 2024, Volume and Issue: 10(30)

Published: July 26, 2024

The three-dimensional (3D) organization of chromatin within the nucleus is crucial for gene regulation. However, 3D architectural features that coordinate activation an entire chromosome remain largely unknown. We introduce omics method, RNA-associated DNA-DNA interactions, integrates RNA polymerase II (RNAPII)–mediated regulome with stochastic optical reconstruction microscopy to investigate landscape noncoding roX2 -associated topology equalization achieve dosage compensation. Our findings reveal anchors target transcription end sites (TESs) and spreads in a distinctive boot-shaped configuration, promoting more open state hyperactivation. Furthermore, arches TES start enhance transcriptional loops, potentially facilitating RNAPII convoying connecting proximal promoter-promoter hubs synergistic These TESs cluster as compartments, surrounded by inactive domains coactivation multiple genes territory. In addition, structures gradually form scaffold stepwise

Language: Английский

Citations

1
The impact of social partners: investigating mixed-strain housing effects on aging in female mice DOI

Chih-Lin Lee,

Yu-Chiao Lin, Tsung-Han Kuo

et al.

Biogerontology, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 11, 2024

Language: Английский

Citations

0
IMPACT OF THE TEMPERATURE ON LARVAL STAGE OF DEVELOPMENT ON THE EXPRESSION LEVELS OF HSP70, INR, SIRT1, MTOR AND FOXO GENESIN MALES AND FEMALES OF DROSOPHILA MELANOGASTER DOI Creative Commons
H. S. Karaman, Alexander Vaiserman, K. S. Afanasieva

et al.

Bulletin of Taras Shevchenko National University of Kyiv Series Biology, Journal Year: 2024, Volume and Issue: 96(1), P. 15 - 23

Published: Jan. 1, 2024

Background. Despite the progress in understanding phenomenon of aging, key factors that influence this process remain poorly understood. Aging is a genetically programmed set events, leading to structural and functional changes reduce life expectancy an organism. The relevance study expand impact environmental factors, particular temperature, on early stages development imago, using Drosophila melanogaster as model. aim was determine analyze expression level genes associated with lifespan D. – Hsp70, InR, Sirt1, mTor foxo flies reared at different temperatures larval stage development. Methods. larvae were kept temperatures, after which gene determined by RT-qPCR adult flies. relative calculated 2-ΔΔCt method. data analyzed ANOVA-test followed pairwise multiple comparison post-hoc Tukey HSD test. Differences considered significant p˂0.05. Results. temperature did not significantly affect male adults. Аt same time females imago increase Sirt1 observed individuals took 20°C 30°C, compared control 25°C. Conclusions. increased levels chosen for analysis under critical conditions indicates induction generalized stress response correlate expectancy. finding sex differences patterns requires further investigation uncover molecular mechanisms underlying it.

Language: Английский

Citations

0
Latitudinal clines in climate and sleep patterns shape disease outcomes in Drosophila melanogaster infected by Metarhizium anisopliae DOI Open Access

Mintong Nan,

Jonathan B. Wang,

Michail Siokis

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 14, 2024

Abstract Major latitudinal clines have been observed in Drosophila melanogaster , a human commensal that originated tropical Africa and has spread world-wide to colonize temperate habitats. However, despite the significant impact of pathogens on species distribution, influence geographical factors disease susceptibility remains poorly understood. This study investigated effects biomes resistance using common fly pathogen Metarhizium anisopliae 43 global populations. The results showed was correlated with gradients sleep duration, temperature humidity. While fungal diversity at latitudes may drive enhanced defenses, most disease-resistant males were also susceptible desiccation, indicating potential trade-offs between abiotic stress resistance, necessary for survival habitats, resistance. found sex, mating status, interacted stresses longer-sleeping virgin flies surviving infections longer, extra daytime post-infection being protective, especially resistant lines. These findings promote idea defense against are intertwined traits related organismal fitness subject joint clinal evolution. Figure

Language: Английский

Citations

0
Sexually dimorphic ATF4 expression in the fat confers female stress tolerance inDrosophila melanogaster DOI Creative Commons
Lydia Grmai, Melissa Mychalczuk,

Aditya Arkalgud

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 27, 2024

Abstract Metabolic differences between males and females have been well documented across many species. However, the molecular basis of these how they impact tolerance to nutrient deprivation is still under investigation. In this work, we use Drosophila melanogaster demonstrate that sex-specific in fat tissue metabolism are driven, part, by dimorphic expression Integrated Stress Response (ISR) transcription factor, ATF4. We found female tissues higher ATF4 activity than their male counter parts homeostatic conditions. This dimorphism was partly due a bias transcript abundance specific splice isoforms. canonical sex determinants transformer ( tra ) doublesex dsx drive such abundance. These persist genetic model deprivation, where animals showed greater resistance lethality an ATF4-dependent manner. results suggest confers stress females. Together, our data describe previously unknown facet ISR signaling wherein sexual identity adipose differential Since energy storage mechanisms known be linked regulation, studies provide mechanistic starting point for understanding influences metabolic disease outcomes.

Language: Английский

Citations

0
Investigation of potential cytotoxicity of a water-soluble, red-fluorescent [70]fullerene nanomaterial in Drosophila melanogaster DOI
Magdalena Rost‐Roszkowska, Anna Z. Urbisz, Karol Małota

et al.

Nanotoxicology, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 16

Published: Dec. 30, 2024

Fullerenes (C

Language: Английский

Citations

0
Allopatric populations of Drosophila ananassae exhibit different degree of trade-off for evolving immune response against bacterial infection DOI Creative Commons
Pallab Paul, Goutam Mukherjee, Sayarneel Mandal

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 29, 2023

Abstract We conducted laboratory based selection experiment with two allopatric populations of Drosophila ananassae collected from isolated geographic regions India namely, Mysuru and Port Blair subsequently inbred for several generations at laboratory. The were infected enteritically the bacteria Pseudomonas aeruginosa fifty-five followed by ten under relaxed selection. tested development immune-competence selected flies through inquired if elicitation immune responses needed to pay cost trading-off life history morphological traits. observed gradual progressive increase in longevity, reproductive span, post developmental time, fecundity rate, fat content reduction body length water content. Under regime there was a reversion all traits but not basal level. Responses exhibited infected-selected differed, former group more length, content, greater antioxidant enzyme activity, longer infection period, survival than later group. On other side, showed deposition, faster oviposition time flies. Interestingly, exhibited, ovipository period contrast that decrease same. inferred D. show different trade-off evolving response

Language: Английский

Citations

0