Environment International,
Journal Year:
2024,
Volume and Issue:
190, P. 108905 - 108905
Published: July 23, 2024
The
emergence
and
dissemination
of
antibiotic
resistance
genes
(ARGs)
in
the
ecosystem
are
global
public
health
concerns.
One
Health
emphasizes
interconnectivity
between
different
habitats
seeks
to
optimize
animal,
human,
environmental
health.
However,
information
on
within
complex
microbiomes
natural
is
scarce.
We
investigated
prevalence
resistant
bacteria
(ARB)
spread
ARGs
intensive
bullfrog
(Rana
catesbeiana)
farms
Shantou
area
China.
Antibiotic
susceptibilities
361
strains,
combined
with
microbiome
analyses,
revealed
Escherichia
coli,
Edwardsiella
tarda,
Citrobacter
Klebsiella
sp.
as
prevalent
multidrug
these
farms.
Whole
genome
sequencing
95
ARB
identified
250
large
plasmids
that
harbored
a
wide
range
ARGs.
Plasmid
sequences
sediment
metagenomes
an
abundance
tetA,
sul1,
aph(3″)-Ib
Notably,
(against
15
antibiotics)
highly
correlated
plasmid-borne
rather
than
chromosome-borne
Based
sequence
similarities,
most
(62%)
fell
into
32
distinct
groups,
indicating
potential
for
horizontal
plasmid
transfer
(HPT)
frog
farm
microbiome.
HPT
was
confirmed
inter-
intra-species
conjugation
experiments.
Furthermore,
identical
mobile
ARGs,
flanked
by
genetic
elements
(MGEs),
were
found
locations
same
plasmid,
or
residing
hosts.
Our
results
suggest
synergy
MGEs
facilitate
Mining
databases
retrieved
similar
from
bacterial
species
other
niches
globally.
findings
underscore
importance
mediating
ecosystem.
International Journal of Molecular Sciences,
Journal Year:
2024,
Volume and Issue:
25(2), P. 1257 - 1257
Published: Jan. 19, 2024
Early
and
accurate
diagnoses
of
pathogenic
microorganisms
is
essential
to
correctly
identify
diseases,
treating
infections,
tracking
disease
outbreaks
associated
with
microbial
develop
precautionary
measures
that
allow
a
fast
effective
response
in
epidemics
pandemics,
thus
improving
public
health.
Aptamers
are
class
synthetic
nucleic
acid
molecules
the
potential
be
used
for
medical
purposes,
since
they
can
directed
towards
any
target
molecule.
Currently,
use
aptamers
has
increased
because
useful
tool
detection
specific
targets.
We
present
brief
review
detect
bacteria
or
even
some
toxins
clinical
importance.
This
work
describes
advances
technology
aptamers,
purpose
providing
knowledge
new
treatment
different
diseases
caused
by
infectious
microorganisms.
The
study
presented
as
an
object
of
research
the
relationship
between
a
charity
hospital
management
and
public
health
services.The
delimitation
this
aims
to
collect
data
relevant
philanthropy
with
emphasis
on
historical
trajectory
Hospital
its
interaction
services.We
also
intend
reconstruct
that
maintains
service
contracts
state
government
Holy
Spirit;
identify
key
milestones
role
forms
used
by
hospital,
in
social
practices
,
discuss
issue
nonprofit
sector
institution.Based
thematic
analysis
found
largest
source
income
is
through
contractual
which
called
partnership.This
marked
conflict
can
be
attributed
limited
picture
these
organizations
for
their
ability
respond
effectively
funder
regulator
processes.The
results
reach
expected
since
there
increase
supply
improvement
population's
access
institution
field
has
contributed
part
process
inclusion.The
future
durability
still
unclear
because
are
many
questions
about
topic.
The
study
presented
as
an
object
of
research
the
relationship
between
a
charity
hospital
management
and
public
health
services.The
delimitation
this
aims
to
collect
data
relevant
philanthropy
with
emphasis
on
historical
trajectory
Hospital
its
interaction
services.We
also
intend
reconstruct
that
maintains
service
contracts
state
government
Holy
Spirit;
identify
key
milestones
role
forms
used
by
hospital,
in
social
practices
,
discuss
issue
nonprofit
sector
institution.Based
thematic
analysis
found
largest
source
income
is
through
contractual
which
called
partnership.This
marked
conflict
can
be
attributed
limited
picture
these
organizations
for
their
ability
respond
effectively
funder
regulator
processes.The
results
reach
expected
since
there
increase
supply
improvement
population's
access
institution
field
has
contributed
part
process
inclusion.The
future
durability
still
unclear
because
are
many
questions
about
topic.
The
study
presented
as
an
object
of
research
the
relationship
between
a
charity
hospital
management
and
public
health
services.The
delimitation
this
aims
to
collect
data
relevant
philanthropy
with
emphasis
on
historical
trajectory
Hospital
its
interaction
services.We
also
intend
reconstruct
that
maintains
service
contracts
state
government
Holy
Spirit;
identify
key
milestones
role
forms
used
by
hospital,
in
social
practices
,
discuss
issue
nonprofit
sector
institution.Based
thematic
analysis
found
largest
source
income
is
through
contractual
which
called
partnership.This
marked
conflict
can
be
attributed
limited
picture
these
organizations
for
their
ability
respond
effectively
funder
regulator
processes.The
results
reach
expected
since
there
increase
supply
improvement
population's
access
institution
field
has
contributed
part
process
inclusion.The
future
durability
still
unclear
because
are
many
questions
about
topic.
The
study
presented
as
an
object
of
research
the
relationship
between
a
charity
hospital
management
and
public
health
services.The
delimitation
this
aims
to
collect
data
relevant
philanthropy
with
emphasis
on
historical
trajectory
Hospital
its
interaction
services.We
also
intend
reconstruct
that
maintains
service
contracts
state
government
Holy
Spirit;
identify
key
milestones
role
forms
used
by
hospital,
in
social
practices
,
discuss
issue
nonprofit
sector
institution.Based
thematic
analysis
found
largest
source
income
is
through
contractual
which
called
partnership.This
marked
conflict
can
be
attributed
limited
picture
these
organizations
for
their
ability
respond
effectively
funder
regulator
processes.The
results
reach
expected
since
there
increase
supply
improvement
population's
access
institution
field
has
contributed
part
process
inclusion.The
future
durability
still
unclear
because
are
many
questions
about
topic.
The
study
presented
as
an
object
of
research
the
relationship
between
a
charity
hospital
management
and
public
health
services.The
delimitation
this
aims
to
collect
data
relevant
philanthropy
with
emphasis
on
historical
trajectory
Hospital
its
interaction
services.We
also
intend
reconstruct
that
maintains
service
contracts
state
government
Holy
Spirit;
identify
key
milestones
role
forms
used
by
hospital,
in
social
practices
,
discuss
issue
nonprofit
sector
institution.Based
thematic
analysis
found
largest
source
income
is
through
contractual
which
called
partnership.This
marked
conflict
can
be
attributed
limited
picture
these
organizations
for
their
ability
respond
effectively
funder
regulator
processes.The
results
reach
expected
since
there
increase
supply
improvement
population's
access
institution
field
has
contributed
part
process
inclusion.The
future
durability
still
unclear
because
are
many
questions
about
topic.
The
study
presented
as
an
object
of
research
the
relationship
between
a
charity
hospital
management
and
public
health
services.The
delimitation
this
aims
to
collect
data
relevant
philanthropy
with
emphasis
on
historical
trajectory
Hospital
its
interaction
services.We
also
intend
reconstruct
that
maintains
service
contracts
state
government
Holy
Spirit;
identify
key
milestones
role
forms
used
by
hospital,
in
social
practices
,
discuss
issue
nonprofit
sector
institution.Based
thematic
analysis
found
largest
source
income
is
through
contractual
which
called
partnership.This
marked
conflict
can
be
attributed
limited
picture
these
organizations
for
their
ability
respond
effectively
funder
regulator
processes.The
results
reach
expected
since
there
increase
supply
improvement
population's
access
institution
field
has
contributed
part
process
inclusion.The
future
durability
still
unclear
because
are
many
questions
about
topic.
The
study
presented
as
an
object
of
research
the
relationship
between
a
charity
hospital
management
and
public
health
services.The
delimitation
this
aims
to
collect
data
relevant
philanthropy
with
emphasis
on
historical
trajectory
Hospital
its
interaction
services.We
also
intend
reconstruct
that
maintains
service
contracts
state
government
Holy
Spirit;
identify
key
milestones
role
forms
used
by
hospital,
in
social
practices
,
discuss
issue
nonprofit
sector
institution.Based
thematic
analysis
found
largest
source
income
is
through
contractual
which
called
partnership.This
marked
conflict
can
be
attributed
limited
picture
these
organizations
for
their
ability
respond
effectively
funder
regulator
processes.The
results
reach
expected
since
there
increase
supply
improvement
population's
access
institution
field
has
contributed
part
process
inclusion.The
future
durability
still
unclear
because
are
many
questions
about
topic.
The
study
presented
as
an
object
of
research
the
relationship
between
a
charity
hospital
management
and
public
health
services.The
delimitation
this
aims
to
collect
data
relevant
philanthropy
with
emphasis
on
historical
trajectory
Hospital
its
interaction
services.We
also
intend
reconstruct
that
maintains
service
contracts
state
government
Holy
Spirit;
identify
key
milestones
role
forms
used
by
hospital,
in
social
practices
,
discuss
issue
nonprofit
sector
institution.Based
thematic
analysis
found
largest
source
income
is
through
contractual
which
called
partnership.This
marked
conflict
can
be
attributed
limited
picture
these
organizations
for
their
ability
respond
effectively
funder
regulator
processes.The
results
reach
expected
since
there
increase
supply
improvement
population's
access
institution
field
has
contributed
part
process
inclusion.The
future
durability
still
unclear
because
are
many
questions
about
topic.