Wastewater Surveillance of SARS-CoV-2 in Zambia: An Early Warning Tool DOI Open Access
Doreen Mainza Shempela, Walter Muleya, Steward Mudenda

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(16), P. 8839 - 8839

Published: Aug. 14, 2024

Wastewater-based surveillance has emerged as an important method for monitoring the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). This study investigated presence of SARS-CoV-2 in wastewater Zambia. We conducted a longitudinal Copperbelt and Eastern provinces Zambia from October 2023 to December during which 155 samples were collected. The subjected three different concentration methods, namely bag-mediated filtration, skimmed milk flocculation, polythene glycol-based assays. Molecular detection nucleic acid was using real-time Polymerase Chain Reaction (PCR). Whole genome sequencing Illumina COVIDSEQ assay. Of samples, 62 (40%) tested positive SARS-CoV-2. these, 13 sequences sufficient length determine lineages obtained phylogenetically analyzed. Various Omicron subvariants detected including BA.5, XBB.1.45, BA.2.86, JN.1. Some these have been clinical cases Interestingly, phylogenetic analysis positioned sequence Province B.1.1.529 clade, suggesting that earlier variants late 2021 could still be circulating may not wholly replaced by newer subvariants. stresses need integrating into mainstream strategies circulation

Language: Английский

Wastewater-Based Epidemiology for SARS-CoV-2 in Northern Italy: A Spatiotemporal Model DOI Open Access

Matilde Fondriest,

Lorenzo Vaccari,

Federico Aldrovandi

et al.

International Journal of Environmental Research and Public Health, Journal Year: 2024, Volume and Issue: 21(6), P. 741 - 741

Published: June 6, 2024

The study investigated the application of Wastewater-Based Epidemiology (WBE) as a tool for monitoring SARS-CoV-2 prevalence in city northern Italy from October 2021 to May 2023. Based on previously used deterministic model, this proposed variation account population characteristics and virus biodegradation sewer network. model calculated loads corresponding COVID-19 cases over time different areas was validated using healthcare data while considering viral mutations, vaccinations, testing variability. correlation between predicted reported high across three waves that occurred during period considered, demonstrating ability predict relevant fluctuations number cases. did not substantially influence infection rates. Conversely, significantly reduced load reaching wastewater treatment plant, resulting 30% reduction total produced area. This approach can be applied compare values cities with demographics network structures, improving comparability WBE effective surveillance intervention strategies.

Language: Английский

Citations

2
Assessment of environmental factors influencing SARS-CoV-2 in Vietnam's surface water across two years of clinical data DOI Creative Commons

Yadpiroon Siri,

Bikash Malla, Le Thanh Thao

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 957, P. 177449 - 177449

Published: Nov. 19, 2024

Wastewater-based epidemiology (WBE) is an effective, non-invasive method for monitoring the spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) by tracking viral prevalence in water. This study aimed to investigate presence SARS-CoV-2 surface water Vietnam over two years. One-step quantitative reverse transcription polymerase chain reaction (qRT-PCR) assays were employed quantify and its variant-specific mutation sites (G339D/E484A) pepper mild mottle virus (PMMoV) from a total 315 samples (105 per type) compare with reported Coronavirus disease 2019 (COVID-19) cases environmental factors. was detected 38 % (40/105), 43 (45/105), 39 (41/105) Sites A, B, C, respectively, concentrations 3.0-5.6 log

Language: Английский

Citations

2
Wastewater-Based Epidemiology for SARS-CoV-2 in Northern Italy: A Spatiotemporal Model DOI Open Access

Matilde Fondriest,

Lorenzo Vaccari,

Federico Aldrovandi

et al.

Published: April 8, 2024

The study investigated the application of Wastewater-Based Epidemiology (WBE) as a tool for monitoring SARS-CoV-2 prevalence in city Northern Italy from October 2021 to May 2023. Starting widely applied deterministic model, this proposed variation take into account population characteristics and virus biodegradation sewer network. model calculated loads corresponding COVID-19 cases over time different areas was validated with healthcare data while considering viral mutations, vaccinations, testing variability. correlation between predicted reported high across three waves that occurred considered period, demonstrating model’s capacity predict relevant fluctuations number cases. Population did not substantially influence infection rates. Conversely, significantly reduced load reaching wastewater treatment plant, resulting 30% reduction total produced area. This approach can be comparing values cities varying demographics network structures, enhancing comparability WBE effective surveillance intervention strategies.

Language: Английский

Citations

1
Wastewater Surveillance of SARS-CoV-2 in Zambia: An Early Warning Tool DOI Open Access
Doreen Mainza Shempela, Walter Muleya, Steward Mudenda

et al.

Published: June 26, 2024

Wastewater-based surveillance has emerged as an important method for monitoring SARS-CoV-2. This study investigated the presence of SARS-CoV-2 in wastewater Zambia. We conducted a longitudinal Copperbelt and Eastern provinces Zambia from October 2023 to December during which 155 samples were collected. The subjected three different concentration methods, namely bag-mediated filtration, skimmed milk flocculation, polythene glycol-based assays. Molecular detection nucleic acid was using real-time PCR. Whole genome sequencing Illumina COVIDSEQ assay. Of samples, 62 (40%) tested positive these, 13 sequences sufficient length determine lineages obtained two phylogenetically analyzed. Various Omicron subvariants detected including BA.5, XBB.1.45, BA.2.86, JN.1. Some these have been clinical cases Interestingly, phylogenetic analysis positioned sequence Province B.1.1.529 clade, suggesting that earlier variants late 2021 could still be circulating may not wholly replaced by newer subvariants. stresses need integrating into mainstream strategies circulation

Language: Английский

Citations

1
Wastewater Monitoring During the COVID-19 Pandemic in the Veneto Region, Italy: Longitudinal Observational Study DOI Creative Commons
Honoria Ocagli,

Marco Zambito,

Filippo Da Re

et al.

JMIR Public Health and Surveillance, Journal Year: 2024, Volume and Issue: 11, P. e58862 - e58862

Published: Sept. 2, 2024

As the COVID-19 pandemic has affected populations around world, there been substantial interest in wastewater-based epidemiology (WBE) as a tool to monitor spread of SARS-CoV-2. This study investigates use WBE anticipate trends by analyzing correlation between viral RNA concentrations wastewater and reported cases Veneto region Italy. We aimed evaluate effectiveness cumulative sum (CUSUM) control chart method detecting changes SARS-CoV-2 its potential an early warning system for outbreaks. Additionally, we validate these findings over different time periods ensure robustness. analyzed temporal clinical outcomes, including confirmed cases, hospitalizations, intensive care unit (ICU) admissions, from October 2021 August 2022 region, Wastewater samples were collected weekly 10 treatment plants using reverse transcription-quantitative polymerase chain reaction. The CUSUM was used detect significant shifts data, with initial analysis conducted February 2022, followed validation second period 2022. found that peaks consistently preceded 5.2 days. Hospitalizations delay 4.25 days, while ICU admissions exhibited lead approximately 6 Notably, certain health districts stronger correlations, notable values anticipating average 13.5 9.5 days 2 specific districts. charts effectively identified load, indicating outbreaks before increased. Validation during consistency findings, reinforcing robustness this context. WBE, combined method, offers valuable insight into level community, asymptomatic thus acting precious infectious disease potential.

Language: Английский

Citations

0
Environmental Detection and Monitoring of SARS-CoV-2 for COVID-19 Risk Prediction DOI
Rita R. Colwell, Kyle D. Brumfield, Moiz Usmani

et al.

Springer eBooks, Journal Year: 2024, Volume and Issue: unknown, P. 65 - 77

Published: Jan. 1, 2024

Language: Английский

Citations

0
Wastewater Monitoring During COVID-19 Epidemic: the Experience in the Veneto Region (Italy) (Preprint) DOI
Honoria Ocagli,

Marco Zambito,

Filippo Da Re

et al.

Published: June 27, 2024

BACKGROUND As the COVID-19 pandemic has affected populations around world, researchers are turning to wastewater-based epidemiology (WBE) testing as a potential tool monitor spread of virus. SARS-CoV-2 concentrations in wastewater thought possibly anticipating epidemic curve. OBJECTIVE The study aims examine this association and its use tracking trends. METHODS temporal series confirmed cases was compared with RNA concentration (in terms genomic copies/µL) between 05/10/2021 14/02/2022 Veneto Region, NE Italy. Data from regional databases for cases, hospitalizations, intensive care units (ICU) admissions were used. Cumulative sum (CUSUM) charts used compare series. RESULTS found that an increase viral load samples December 2021 January 2022 followed by positive 5-day delay. peaks anticipated case average 5.2 days. also 4.25-day time detecting notable values certain healthcare districts exhibiting stronger correlations than others. observed limited ICU admissions. However, exhibited correlations, 13.5 9.5 days two specific districts. CONCLUSIONS Wastewater can provide valuable insights into level community, including asymptomatic thus providing precious early-warning infectious disease outbreaks potential.

Language: Английский

Citations

0
Wastewater Surveillance of SARS-CoV-2 in Zambia: An Early Warning Tool DOI Open Access
Doreen Mainza Shempela, Walter Muleya, Steward Mudenda

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(16), P. 8839 - 8839

Published: Aug. 14, 2024

Wastewater-based surveillance has emerged as an important method for monitoring the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). This study investigated presence of SARS-CoV-2 in wastewater Zambia. We conducted a longitudinal Copperbelt and Eastern provinces Zambia from October 2023 to December during which 155 samples were collected. The subjected three different concentration methods, namely bag-mediated filtration, skimmed milk flocculation, polythene glycol-based assays. Molecular detection nucleic acid was using real-time Polymerase Chain Reaction (PCR). Whole genome sequencing Illumina COVIDSEQ assay. Of samples, 62 (40%) tested positive SARS-CoV-2. these, 13 sequences sufficient length determine lineages obtained phylogenetically analyzed. Various Omicron subvariants detected including BA.5, XBB.1.45, BA.2.86, JN.1. Some these have been clinical cases Interestingly, phylogenetic analysis positioned sequence Province B.1.1.529 clade, suggesting that earlier variants late 2021 could still be circulating may not wholly replaced by newer subvariants. stresses need integrating into mainstream strategies circulation

Language: Английский

Citations

0