Basic mechanism of mobilizing cell movement during invasion of glioblastoma and target selection of targeted therapy DOI Creative Commons
Jie Xu, Pengfei Shi, Liqun Yang

et al.

Journal of Advanced Research, Journal Year: 2025, Volume and Issue: unknown

Published: May 1, 2025

Glioblastoma (GBM), also known as glioblastoma multiforme, is a rapidly growing and highly invasive malignant tumor. Due to the inability clearly distinguish between normal tissue, surgery cannot achieve safe resection, often leading poor patient prognosis inevitable tumor recurrence. According previous studies, GBM invasion related intercellular adhesion, matrix degradation, extracellular its adhesion molecules, well molecular of protein hydrolases in microenvironment cells stromal cells. The aim enhance our understanding mechanisms underlying advance research on targeted therapies for inhibiting invasion. This article describes that may affect cell invasion, changes cytoskeleton during motility, regulatory intracellular signaling pathways In addition, we explored possibility therapy against molecules GBM.

Language: Английский

Constructing a Glioblastoma Prognostic Model Related to Fatty Acid Metabolism Using Machine Learning and Identifying F13A1 as a Potential Target DOI Creative Commons
Yu‐Shu Liu, Hui Deng,

Ping Song

et al.

Biomedicines, Journal Year: 2025, Volume and Issue: 13(2), P. 256 - 256

Published: Jan. 21, 2025

Background: Increased fatty acid metabolism (FAM) is an important marker of tumor metabolism. However, the characterization and function FAM-related genes in glioblastoma (GBM) have not been fully explored. Method: In TCGA-GBM cohort, were divided into three clusters (C1, C2, C3), DEGs between those normal group GBM cohort considered key genes. On basis 10 kinds machine learning methods, we used 101 combinations algorithms to construct prognostic models obtain best model. addition, also validated model GSE43378, GSE83300, CGGA, REMBRANDT datasets. We conducted a multifaceted analysis F13A1, which plays role Results: with worst prognosis, may be associated immunosuppressive phenotype, related positive regulation cell adhesion lymphocyte-mediated immunity. Using multiple identified RSF as model, F13A1 accounts for most contribution. can support malignant cells by promoting macrophages, leading poor prognosis patients. This metabolic reprogramming only enhances survival proliferation but promote growth, invasion, metastasis secreting growth factors cytokines. significantly correlated immune-related molecules, including IL2RA, activate immunity, IL10, suggests immune suppression. interferes recognition killing affecting MHC molecules. Conclusions: The developed here helps us further enhance our understanding FAM provides compelling avenue clinical prediction patient treatment. possibly novel macrophages.

Language: Английский

Citations

0
Molecular Mechanisms of Neutrophil Extracellular Traps in Promoting Gastric Cancer Epithelial–Mesenchymal Transition Through SERPINE‐1 Expression DOI Creative Commons
Zhen Ma, Xiaolin Li,

Shifeng Yang

et al.

Journal of Biochemical and Molecular Toxicology, Journal Year: 2025, Volume and Issue: 39(3)

Published: March 1, 2025

Gastric cancer remains a significant global health concern, with its progression and metastasis often associated epithelial-mesenchymal transition (EMT). This study investigated the role of neutrophil extracellular traps (NETs) in promoting gastric EMT by regulating SERPINE-1 expression, which encodes plasminogen activator inhibitor-1 (PAI-1). Western blot immunohistochemistry were used to detect protein expression. Cell Counting Kit-8 was tested for cell proliferation ability using clones. The gene knocked down lentivirus. Immunofluorescence co-expression proteins, Transwell assay wound-healing investigate migration cells. Experimental conclusions verified vivo nude mouse model. We first demonstrated overexpression PAI-1 tissues lines. Subsequently, we found that NETs significantly enhanced expression EMT-related markers. These changes accompanied increases invasion, migration, tumour sphere formation. To further elucidate mechanism, employed lentivirus-mediated knockdown reverse NET-induced phenotype effectively. Mechanistically, activated transforming growth factor (TGF)-β signalling pathway via as evidenced increased TGF-β1, TGF-βR1, TGF-βR2, phosphorylated Smad2/3 Smad4. Finally, experiments model liver confirmed NET-treated HGC-27 cells exhibited metastatic potential abrogated potential. Our findings reveal novel mechanism promote PAI-1-TGF-β axis. can be target treatment cancer, is closely related prognosis patients cancer. Therapeutic strategies targeting or may help prevent improve clinical outcomes patients.

Language: Английский

Citations

0
Caveolae: Metabolic Platforms at the Crossroads of Health and Disease DOI Open Access

David Stea,

Alessio D’Alessio

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(7), P. 2918 - 2918

Published: March 24, 2025

Caveolae are small flask-shaped invaginations of the plasma membrane enriched in cholesterol and sphingolipids. They play a critical role various cellular processes, including signal transduction, endocytosis, mechanotransduction. Caveolin proteins, specifically Cav-1, Cav-2, Cav-3, addition to their as structural components caveolae, have been found regulate activity signaling molecules. A growing body research has highlighted pivotal caveolae caveolins maintaining metabolic homeostasis. Indeed, studies demonstrated that interact with key insulin signaling, glucose uptake, lipid metabolism, thereby influencing energy production storage. The dysfunction or altered expression associated disorders, obesity, type 2 diabetes, ocular diseases. Remarkably, mutations caveolin genes can disrupt balance, promote oxidative stress, exacerbate dysregulation. This review examines current on molecular mechanisms through which explores involvement pathogenesis discusses potential therapeutic strategies targeting function stabilization restore

Language: Английский

Citations

0
Deep learning assisted identification of SCUBE2 and SLC16 A5 combination in RNA-sequencing data as a novel specific potential diagnostic biomarker in prostate cancer DOI

Saeideh Khorshid Sokhangouy,

Mohsen Zeinali, Sina Fathi

et al.

Medical & Biological Engineering & Computing, Journal Year: 2025, Volume and Issue: unknown

Published: May 8, 2025

Language: Английский

Citations

0
Basic mechanism of mobilizing cell movement during invasion of glioblastoma and target selection of targeted therapy DOI Creative Commons
Jie Xu, Pengfei Shi, Liqun Yang

et al.

Journal of Advanced Research, Journal Year: 2025, Volume and Issue: unknown

Published: May 1, 2025

Glioblastoma (GBM), also known as glioblastoma multiforme, is a rapidly growing and highly invasive malignant tumor. Due to the inability clearly distinguish between normal tissue, surgery cannot achieve safe resection, often leading poor patient prognosis inevitable tumor recurrence. According previous studies, GBM invasion related intercellular adhesion, matrix degradation, extracellular its adhesion molecules, well molecular of protein hydrolases in microenvironment cells stromal cells. The aim enhance our understanding mechanisms underlying advance research on targeted therapies for inhibiting invasion. This article describes that may affect cell invasion, changes cytoskeleton during motility, regulatory intracellular signaling pathways In addition, we explored possibility therapy against molecules GBM.

Language: Английский

Citations

0