New Highly Sensitive Methods for Electroanalysis of the Catalytic Activity of Enzymes of Medical Significance DOI Open Access
Victoria V. Shumyantseva, L.E. Agafonova,

Tatiana V. Bulko

et al.

Biomedical Chemistry Research and Methods, Journal Year: 2024, Volume and Issue: 7(3), P. e00225 - e00225

Published: Jan. 1, 2024

The review is devoted to new highly effective methods for analyzing the catalytic activity of enzymes medical significance, such as cytochromes P450, trypsin, asparaginase, beta-lactamase, and nucleases. are based on registration specific using electroanalytical methods. analyzes experimental data obtained by authors. Two platforms have been developed that allow quantitative measurement electrochemical properties enzyme (cytochrome bactosomes, asparaginase) or substrate (trypsin, nucleases, restriction enzymes, beta-lactamase).

Language: Английский

Quantitative Analysis of Mixed DNA Proportions from Two Donors DOI

Tianli Guo,

Guan Hua

Published: Jan. 10, 2025

Language: Английский

Citations

0
Carbon Quantum Dots in the Electroanalysis of DNA and Albumin DOI

Lyubov E. Agafonova,

Tatiana V. Bulko,

Veronica V. Pronina

et al.

BioNanoScience, Journal Year: 2025, Volume and Issue: 15(3)

Published: May 21, 2025

Language: Английский

Citations

0
Electrochemical profiling of poliovirus particles inactivated by chemical method and ionizing radiation DOI
L.E. Agafonova, Victoria V. Shumyantseva,

Yu.Yu. Ivin

et al.

Biomeditsinskaya Khimiya, Journal Year: 2024, Volume and Issue: 70(3), P. 161 - 167

Published: Jan. 1, 2024

Electrochemical profiling of formaldehyde-inactivated poliovirus particles demonstrated a relationship between the D-antigen concentration and intensity maximum amplitude currents samples. The resultant signal was therefore identified as electrochemical oxidation surface proteins poliovirus. Using registration electrooxidation amino acid residues capsid proteins, comparative analysis inactivated by electrons accelerated with doses 5 kGy, 10 15 25 30 kGy at room temperature carried out. An increase in radiation dose accompanied an signals. A significant signals detected upon irradiation 15-30 kGy. data obtained suggest that change profile are associated degree structural reorganization insufficient preservation under these conditions inactivation.

Language: Английский

Citations

0
New Highly Sensitive Methods for Electroanalysis of the Catalytic Activity of Enzymes of Medical Significance DOI Open Access
Victoria V. Shumyantseva, L.E. Agafonova,

Tatiana V. Bulko

et al.

Biomedical Chemistry Research and Methods, Journal Year: 2024, Volume and Issue: 7(3), P. e00225 - e00225

Published: Jan. 1, 2024

The review is devoted to new highly effective methods for analyzing the catalytic activity of enzymes medical significance, such as cytochromes P450, trypsin, asparaginase, beta-lactamase, and nucleases. are based on registration specific using electroanalytical methods. analyzes experimental data obtained by authors. Two platforms have been developed that allow quantitative measurement electrochemical properties enzyme (cytochrome bactosomes, asparaginase) or substrate (trypsin, nucleases, restriction enzymes, beta-lactamase).

Language: Английский

Citations

0