A pan‐cancer analysis of the oncogenic and immunological roles of THOC3 in human cancer DOI Creative Commons
Jixin Zhang, Qi Zhao, Jidong Zhao

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 14, 2024

Abstract Background: There is a limited number of studies on THO Complex Subunit 3 (THOC3) in tumors. The purpose this study to conduct comprehensive analysis various types tumors determine the role THOC3 tumor progression and investigate its impact immunity. Methods. Retrieved expression data from cancers TCGA database analyzed it using R software (version 3.6.4) related packages; explored differential tumors, correlation with prognosis, functional enrichment, relationship heterogeneity. also aimed uncover between Results. THOC3 differentially expressed normal samples, correlated overall survival progression-free time. found that strongly associated mutational burden, microsatellite deletion, immune response. significantly cell infiltration, can regulate transcription output mRNA splicing. Therefore, we speculate could serve as therapeutic target for future anticancer therapies. Conclusions. may novel specific biomarker prognosis immunotherapy.

Language: Английский

Mechano-mediated M2 macrophage polarization and immune suppression in stiffened tumor microenvironment DOI Open Access
Jiranuwat Sapudom, Paul Sean Tipay, Jeremy Teo

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 29, 2024

Abstract The tumor microenvironment (TME), which is composed of various cell types and the extracellular matrix (ECM), plays crucial roles in cancer progression treatment outcomes. However, impact mechanical properties ECM, specifically collagen fibril alignment crosslinking, on macrophage behavior polarization less understood. To investigate this, we reconstituted 3D matrices to mimic physical characteristics TME. Our results demonstrated that stiffening through or crosslinking fibrils promotes toward anti-inflammatory M2 phenotype. This phenotype characterized by increased expression CD105 CD206 a distinct cytokine secretion profile. stiffness aligned activate mechanotransduction pathways, notably integrin β1 PI3K signaling, leading IL-4 secretion, acts an autocrine manner further promote polarization. Interestingly, these stiffened microenvironments also suppressed proinflammatory response. In coculture experiments with breast lines (MDA-MB-231 MCF-7), macrophages within significantly proliferation invasion. These findings suggest its create more favorable environment for modulating activity. Overall, our study underscores critical role ECM mechanics shaping immune TME, highlighting potential therapies target inhibit enhance efficacy.

Language: Английский

Citations

0
A pan‐cancer analysis of the oncogenic and immunological roles of THOC3 in human cancer DOI Creative Commons
Jixin Zhang, Qi Zhao, Jidong Zhao

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 14, 2024

Abstract Background: There is a limited number of studies on THO Complex Subunit 3 (THOC3) in tumors. The purpose this study to conduct comprehensive analysis various types tumors determine the role THOC3 tumor progression and investigate its impact immunity. Methods. Retrieved expression data from cancers TCGA database analyzed it using R software (version 3.6.4) related packages; explored differential tumors, correlation with prognosis, functional enrichment, relationship heterogeneity. also aimed uncover between Results. THOC3 differentially expressed normal samples, correlated overall survival progression-free time. found that strongly associated mutational burden, microsatellite deletion, immune response. significantly cell infiltration, can regulate transcription output mRNA splicing. Therefore, we speculate could serve as therapeutic target for future anticancer therapies. Conclusions. may novel specific biomarker prognosis immunotherapy.

Language: Английский

Citations

0