Mechanical stress facilitates calcium influx and growth of alveolar epithelial cells via activation of the BDKRB1/Ca2+/CaMKII/MEK1/ERK axis

Respiratory Research, Journal Year: 2025, Volume and Issue: 26(1)

Published: April 28, 2025

Mechanical stress and calcium metabolism are associated with lung development various pulmonary diseases. Our previous research demonstrated that BDKRB1/Ca2+ signal transduction may be involved in dysplasia resulting from scoliosis thoracic insufficiency. Therefore, the present study aims to investigate effects of mechanical on growth influx alveolar epithelial cells, as well role signaling these processes. Flow cytometry, CCK-8, EDU staining assay were employed assess cycle, influx, activity, proliferation RLE-6TN cells subjected stresses varying amplitudes (5%, 10% 15%). RT-qPCR western blotting performed evaluate BDKRB1/Ca2+/CaMKII/MEK1/ERK cells. at effectively enhanced viability, positive ratio, S-phase percentage, Ca2+ concentration while reducing G1-phase percentage. Conversely, 15% exerted an inhibitory effect cell proliferation. Additionally, significantly upregulated expression BDKRB1, CaMKIIα/δ, p-MEK1 p-ERK1/2 Notably, BDKRB1 knockdown attenuated stress-induced increase both Moreover, blocked activation Ca2⁺/CaMKII/MEK1/ERK pathway induced by stress. Appropriate levels contribute modulating signaling.

Language: Английский

Validation of the Korean Version of the 24-Item Early-Onset Scoliosis Questionnaire: A Multicenter Study from the Korean Research Society of Spinal Deformity

Clinics in Orthopedic Surgery, Journal Year: 2025, Volume and Issue: 17(1), P. 123 - 123

Published: Jan. 1, 2025

A 24-item early-onset scoliosis questionnaire (EOSQ-24) has been developed as a valid tool for assessing the physical and emotional function of patients with (EOS). Previous studies that conducted transcultural adaptation original EOSQ-24 into other languages have demonstrated high reliability questionnaire. However, Korean version is not available, limiting optimal patient assessment in this nation. Therefore, study was to develop validate EOSQ-24. The English cross-culturally adapted following standard guidelines. final prospectively applied group who were native speakers. Internal consistency evaluated using Cronbach α coefficient item-total correlations. mean, deviation, floor effect, ceiling effect each item also assessed. total 102 caregivers EOS (45 males 57 females) completed patients' average age 6.39 ± 2.16 years. scale 0.942, indicating excellent reliability. corrected correlation coefficients ranged between 0.449 0.788. mean value 3.19. Floor effects all questionnaires below 30%, which could be considered acceptable. Our successfully validated EOSQ-24, providing reliable instrument well-being their context. widespread adoption clinical practice research settings can enhance quality care improve outcomes individuals affected by Korea.

Language: Английский

Thrombospondin-1 Airway Expression and Thrombospondin-1 Gene Variants Are Associated with Bronchopulmonary Dysplasia in Extremely Low-Birth-Weight Infants: A Pilot Study

Children, Journal Year: 2025, Volume and Issue: 12(4), P. 424 - 424

Published: March 28, 2025

Thrombospondin-1 (TSP-1) is an extracellular glycoprotein that mediates the differentiation of pulmonary endothelial cells and specialized stem into alveolar epithelial lineage-specific during repair phase after lung injury. Since bronchopulmonary dysplasia (BPD) involves inhibition development with altered structure vasculature, differential expression THBS-1 gene may impact cell have important role in BPD. This prospective single-center cohort study included ELBW infants without DNA from buccal swabs underwent RT-PCR TaqMan probes, TSP-1 protein was measured tracheal aspirates. Statistical analyses used Chi-square tests, Fisher's exact Wilcoxon Rank Sum t-tests (p < 0.05). BPD had significantly lower gestational ages birth weights compared to those [25 (24,26) 27 (25,28) weeks; median (IQR); p = 0.008] [712 (155) 820 (153) grams; mean (SD); 0.002], respectively. There were significant differences haplotype distributions THBS1 variants rs2664139/rs1478604 0.006) rs1478605/rs1478604 0.008) between no-BPD groups. also airway levels moderate severe patients [(p 0.02) (no BPD: 527 (114-1755); 312 (262-641); 211: (117-352) ng/dL; (IQR)]. Although no individual differed, two haplotypes early varied by severity, suggesting a for pathogenesis infants.

Language: Английский