Hesperidin Facilitating Gastrointestinal Motility by “Gut-brain axis” and “SCF/C-Kit Signaling Pathways” DOI Creative Commons
Yunfei Li, Xinying Zhou,

Yusong Du

et al.

Poultry Science, Journal Year: 2024, Volume and Issue: 103(12), P. 104390 - 104390

Published: Oct. 9, 2024

Hesperidin shows promising results as a potential feed additive for enhancing gastrointestinal motility in animals. Gastrointestinal function plays pivotal role animal growth and the digestibility of dietary nutrients, with motor serving crucial component. However, limited research has been conducted on application hesperidin to promote motility. The present study aims assess efficacy promoting elucidating its underlying mechanism. A total 200 newly hatched (1-day-old) broilers similar body weight were randomly allocated into 4 groups follows: control group receiving only basal diet, other 3 supplemented 50, 100, 150 mg per kg respectively. Each consisted 5 replicates ten replicate, feeding trial lasted duration 21 d. At d age, 5% w/v Evans Blue solution distilled water was utilized measure intestinal transit rates (ITR). Gastric emptying (GE) evaluated by administering phenol red at concentration 0.05% (1 mL/broiler). Fifteen from each euthanized immediately dissected obtain gizzard, hypothalamus, duodenum, jugular blood samples. Jugular samples collected brain-gut peptide content analysis, while duodenum used immunohistochemical analysis. Real-time qPCR performed gizzard demonstrated significant improvement GE ITR all treatment compared (P < 0.05), particularly 100mg/Kg 150mg/Kg group. Incorporation broilers' diet significantly enhances serum levels ghrelin, encompassing serotonin (5-HT), motilin (MTL), cholecystokinin (CCK), Stem Cell Factor (SCF) well substance P (SP) duodenal tissues reducing vasoactive (VIP) 0.05). administered dosage exhibited most pronounced effect.Immunohistochemistry analysis revealed that supplementation up-regulated SP protein down-regulated VIP effect illustrated Furthermore, addition broiler resulted up-regulation expression gene related SCF Receptor tyrosine kinase (C-Kit) upregulated group, C-Kit 50 mg/Kg In conclusion, exhibits broilers, improves through modulation both "gut-brain axis" signaling pathways "SCF/C-Kit pathways" within chicken's digestive system. Notably, demonstrates superior efficacy.

Language: Английский

Rapid-release and user-friendly costunolide/dehydrocostuslactone hydrophilic nanofibers: Therapeutic effects on acute gastric ulcers DOI
Linzhi Li,

Huifa Ouyang,

Jiaying Wu

et al.

International Journal of Pharmaceutics, Journal Year: 2025, Volume and Issue: 670, P. 125194 - 125194

Published: Jan. 10, 2025

Language: Английский

Citations

0
Comprehensive Investigation of Homology and Heterogeneity Between Aurantii Fructus and Aurantii Fructus Immaturus Using Chemometrics Combined with Network Pharmacology DOI
Jie Gao, Meiqi Liu,

Zijie Yang

et al.

Journal of Medicinal Food, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 30, 2024

Aurantii Fructus (AF) and Immaturus (AFI) are distinct herbs outlined by the Chinese Pharmacopoeia. They sourced from same plant but harvested at different times, resulting in differences efficacy. It is important to avoid mixing them clinically distinguish between two. Furthermore, dissimilar cultivation conditions may cause variability quality of herbs, so it vital differentiate drugs origins. In this study, two plants, AF AFI provinces, were comparatively analyzed based on High Performance Liquid Chromatography (HPLC) fingerprints classified using chemometric methods. The results indicate that medicines can be clearly distinguished. Also, grown locations Ten chemical markers screened, their variations determined, including eriocitrin, narirutin, naringin, meranzin hydrate, naringenin, hesperidin, nobiletin, tangeretin, neohesperidin, poncirin. Subsequent network pharmacology correlated screened components with biological organism. material basis difference efficacy homologous was explored perspective changes composition. This study provides a reference for formulating evaluation standards lays foundation efficacy-related research

Language: Английский

Citations

1
Hesperidin Facilitating Gastrointestinal Motility by “Gut-brain axis” and “SCF/C-Kit Signaling Pathways” DOI Creative Commons
Yunfei Li, Xinying Zhou,

Yusong Du

et al.

Poultry Science, Journal Year: 2024, Volume and Issue: 103(12), P. 104390 - 104390

Published: Oct. 9, 2024

Hesperidin shows promising results as a potential feed additive for enhancing gastrointestinal motility in animals. Gastrointestinal function plays pivotal role animal growth and the digestibility of dietary nutrients, with motor serving crucial component. However, limited research has been conducted on application hesperidin to promote motility. The present study aims assess efficacy promoting elucidating its underlying mechanism. A total 200 newly hatched (1-day-old) broilers similar body weight were randomly allocated into 4 groups follows: control group receiving only basal diet, other 3 supplemented 50, 100, 150 mg per kg respectively. Each consisted 5 replicates ten replicate, feeding trial lasted duration 21 d. At d age, 5% w/v Evans Blue solution distilled water was utilized measure intestinal transit rates (ITR). Gastric emptying (GE) evaluated by administering phenol red at concentration 0.05% (1 mL/broiler). Fifteen from each euthanized immediately dissected obtain gizzard, hypothalamus, duodenum, jugular blood samples. Jugular samples collected brain-gut peptide content analysis, while duodenum used immunohistochemical analysis. Real-time qPCR performed gizzard demonstrated significant improvement GE ITR all treatment compared (P < 0.05), particularly 100mg/Kg 150mg/Kg group. Incorporation broilers' diet significantly enhances serum levels ghrelin, encompassing serotonin (5-HT), motilin (MTL), cholecystokinin (CCK), Stem Cell Factor (SCF) well substance P (SP) duodenal tissues reducing vasoactive (VIP) 0.05). administered dosage exhibited most pronounced effect.Immunohistochemistry analysis revealed that supplementation up-regulated SP protein down-regulated VIP effect illustrated Furthermore, addition broiler resulted up-regulation expression gene related SCF Receptor tyrosine kinase (C-Kit) upregulated group, C-Kit 50 mg/Kg In conclusion, exhibits broilers, improves through modulation both "gut-brain axis" signaling pathways "SCF/C-Kit pathways" within chicken's digestive system. Notably, demonstrates superior efficacy.

Language: Английский

Citations

1