An evaluation of a new rapid qPCR test for the detection of 2019-novel coronavirus nucleocapsid (N1) gene in wastewater in Roanoke and Salem VA sewersheds DOI Creative Commons
Lia Willow Lehrer, A Lewis, S. C. Tolliver

et al.

Journal of Water and Health, Journal Year: 2024, Volume and Issue: 22(8), P. 1419 - 1428

Published: Aug. 1, 2024

ABSTRACT The COVID-19 pandemic initiated public interest in wastewater-based epidemiology (WBE). Public and private entities responded to the need produce timely accurate data. LuminUltra Hach partnered provide a rapid, field-based quantitative polymerase chain reaction (qPCR) test for detecting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) wastewater. This study evaluates GeneCount SARS-CoV-2 Wastewater RT-qPCR Assay Kit GeneCount® Q-16 RT-PCR instrument. methods were compared Promega Wizard® Enviro Total Nucleic Acid kit Bio-Rad CFX Opus 96 Real-time PCR Detection System. Over 12-week period, wastewater samples collected weekly from seven locations Roanoke/Salem, VA sewersheds. Concentration extraction of viral RNA followed by qPCR analysis. target gene detection was nucleocapsid (N1) virus. Costs, ease use, time results, sample preparation, data comparisons considered. comparison determined that method instrument more affordable, consumed less time, required technical expertise. While new specific, it had low sensitivity. evaluation suggests should be reserved limited situations requiring onsite field analysis where accuracy is not essential.

Language: Английский

Epidemiological monitoring of sewage sludge and wastewater from an upflow anaerobic sludge blanket reactor using SARS-CoV-2 as a model DOI Creative Commons
J. Wagner,

Maria Fernanda Rech,

Bruno Aschidamini Prandi

et al.

Water Science & Technology, Journal Year: 2025, Volume and Issue: 91(4), P. 333 - 343

Published: Feb. 4, 2025

ABSTRACT This study explores the potential of sludge-based monitoring from an upflow anaerobic sludge blanket reactor for epidemiological surveillance, using SARS-CoV-2 as a model. We monitored copy numbers and mutations, compared concentrations in to wastewater samples taken on same days. From January August 2021, 32 were analyzed; 30 (93%) positive SARS-CoV-2, varied 0.147 2.314 copies ×106/L. In collected days, 31 (96%) ranged 0.058 3.014 The concentration rose along with confirmed hospitalization cases March, while 2 weeks earlier new cases. Mutations variants concern, Gamma Delta, identified months that they became dominant corresponding regions. Our results indicate that, although sewage was not effective anticipating infection numbers, it is promising way gain insight into situation city or region.

Language: Английский

Citations

0
Wastewater-based epidemiology of SARS-CoV-2 and the development of early warning system: challenges and lessons learned after two years of weekly surveillance in the Brazilian Federal District DOI Creative Commons
Rafaella Silveira, Carla Simone Vizzotto, Carla Patrícia Pereira Alves

et al.

Published: Feb. 1, 2025

Language: Английский

Citations

0
Examining the Impact of Different Scales on COVID-19 Monitoring in Sewerage Catchment Areas: Insights from Latvia DOI Creative Commons
Brigita Dejus, Dita Gudrā, Sandis Dejus

et al.

Journal of Hazardous Materials Advances, Journal Year: 2025, Volume and Issue: unknown, P. 100676 - 100676

Published: March 1, 2025

Language: Английский

Citations

0
Behind the bars: Wastewater-based surveillance of SARS-CoV-2, and its variants in prison wastewater DOI Creative Commons
Siti Aishah Rashid, Khayri Azizi Kamel, Raheel Nazakat

et al.

Heliyon, Journal Year: 2025, Volume and Issue: 11(6), P. e43063 - e43063

Published: March 1, 2025

Language: Английский

Citations

0
Wastewater-Based Epidemiology for Analysis of Human Papillomavirus Infections in a Uruguayan Urban Area DOI

C Fernandez-Sabatella,

Gustavo Corrales,

Lucas Monzón

et al.

Food and Environmental Virology, Journal Year: 2025, Volume and Issue: 17(2)

Published: April 29, 2025

Language: Английский

Citations

0
COVID-19 impact on wind and solar energy sector and cost of energy prediction based on machine learning DOI Creative Commons
Saheb Ghanbari Motlagh, Fatemeh Razi Astaraei, Mohammad Montazeri

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(17), P. e36662 - e36662

Published: Aug. 24, 2024

This study examines the impact of COVID-19 pandemic on renewable energy sectors across seven countries through techno-economic analysis and machine learning (ML). In China, fraction decreased in grid-connected systems due to 14.6 % higher diesel fuel prices. They reduced grid electricity prices, with Cost Energy (COE) reductions driven by a 2.8 inflation decrease 3 discount rate cut. The increase adoption USA during was initial operational costs components, significant rise government policy changes, despite reduction sell-back prices rising capital annual expanded capacity. Canada noted shift standalone 50 lower PV 2 WT 48 cost rise, reducing COE except grid/WT scenarios. Germany managed costs, decreasing inflation. India HRESs sevenfold capacity increase, lowering COE. Japan saw stable minimal variation. Iran faced economic challenges 104 impacting decrease. Machine forecasts suggest that may cause an China effects.

Language: Английский

Citations

1
An evaluation of a new rapid qPCR test for the detection of 2019-novel coronavirus nucleocapsid (N1) gene in wastewater in Roanoke and Salem VA sewersheds DOI Creative Commons
Lia Willow Lehrer, A Lewis, S. C. Tolliver

et al.

Journal of Water and Health, Journal Year: 2024, Volume and Issue: 22(8), P. 1419 - 1428

Published: Aug. 1, 2024

ABSTRACT The COVID-19 pandemic initiated public interest in wastewater-based epidemiology (WBE). Public and private entities responded to the need produce timely accurate data. LuminUltra Hach partnered provide a rapid, field-based quantitative polymerase chain reaction (qPCR) test for detecting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) wastewater. This study evaluates GeneCount SARS-CoV-2 Wastewater RT-qPCR Assay Kit GeneCount® Q-16 RT-PCR instrument. methods were compared Promega Wizard® Enviro Total Nucleic Acid kit Bio-Rad CFX Opus 96 Real-time PCR Detection System. Over 12-week period, wastewater samples collected weekly from seven locations Roanoke/Salem, VA sewersheds. Concentration extraction of viral RNA followed by qPCR analysis. target gene detection was nucleocapsid (N1) virus. Costs, ease use, time results, sample preparation, data comparisons considered. comparison determined that method instrument more affordable, consumed less time, required technical expertise. While new specific, it had low sensitivity. evaluation suggests should be reserved limited situations requiring onsite field analysis where accuracy is not essential.

Language: Английский

Citations

0