Environmental Pollution, Journal Year: 2024, Volume and Issue: 363, P. 125118 - 125118
Published: Oct. 14, 2024
Language: Английский
Free Radical Biology and Medicine, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 1, 2025
Language: Английский
Citations
0
Journal of Biochemical and Molecular Toxicology, Journal Year: 2025, Volume and Issue: 39(3)
Published: Feb. 23, 2025
ABSTRACT This study aimed to investigate the impact of in vitro low‐dose arachidonic acid (AA) addition on enhancing sperm motility obese infertile men with asthenozoospermia. Semen samples were collected from 115 men, categorized into two BMI groups: 18.5–23.9 kg/m 2 and ≥ 28 , all subjects demonstrating a concentration 15 × 10 6 /mL. These further divided four cohorts based percentage progressive (PR): control‐normal, control‐asthenozoospermia, obese‐normal, obese‐asthenozoospermia. Normal PR was classified as 32%, while asthenozoospermia characterized by < 32%. Metabolomic analysis employed quantify seminal plasma metabolites, differential metabolites identified through statistical evaluation. Additionally, semen men—5 body mass index (BMI) 5 —underwent scrutiny. Post‐initial analysis, 1 mL stock extracted, treated 100 pg AA, incubated at 37°C for h, reanalyzed determine motility. 16 Sprague Dawley (SD) rats split control obese. The group received standard diet, subjected 45% high‐fat diet. After 3 months, euthanized via cervical dislocation, their prostate vesicles metabolite analysis. A comprehensive 4635 revealed that bile secretion emerged most significant pathway within organic systems category, accounting 0.6% total metabolites. Meanwhile, metabolic pathways overwhelmingly dominated metabolism AA contributing 4.62%. Notably, 29 associated secretion, yet no differences observed between 32% groups. In contrast, 214 linked metabolism, exhibiting predominantly downregulated trend, upregulated identified. Within network, indicators showed positive association induced acrosome reaction, Ca 2+ levels, PR, proportion grade (rapid forward motion, speed 25 μm/s). secretory phospholipase A2 (sPLA2), cyclooxygenase‐1 (COX1) levels demonstrated negative correlation anthropometric measurement parameters Control‐SP group, though this did not reach significance, evident Obesity‐SP group. concentrations sPLA2, COX1 network exhibited following trend: Control‐SP‐N > Obesity‐SP‐N Control‐SP‐A Obesity‐SP‐A. significantly enhanced B (slow‐moving, μm/s) reducing C (non‐forward‐moving) individuals ( p 0.05). those marked increase corresponding reduction noted Human elevated compared However, vesicle SD differ Control Obesity groups Distinct profiles stratified BMI, exhibit impacts quality. Low‐dose under physiological conditions, maintains integrity augments fertilization potential. administration demonstrates superior effectiveness parameters, particularly
Language: Английский
Citations
0
Biology of Reproduction, Journal Year: 2024, Volume and Issue: 111(5), P. 977 - 986
Published: Aug. 23, 2024
Abstract Environmental pollution is an inevitable ecological issue accompanying the process of socialization, with increasing attention to its impacts on individual organisms and chains. The reproductive system, responsible for transmitting genetic material in animals, one most sensitive systems environmental toxins. Research reveals that Sertoli cells are primary target action Different toxins mostly affect blood–testis barrier lead male disorders by disrupting cells. Therefore, this article provides in-depth exploration toxic mechanisms various types testes. It dynamic processes tight junctions affected their specific roles reconstruction process.
Language: Английский
Citations
2
Environmental Pollution, Journal Year: 2024, Volume and Issue: 363, P. 125118 - 125118
Published: Oct. 14, 2024
Language: Английский
Citations
1