MutL Significantly Regulates the Formation of Biofilms in Bacillus amyloliquefaciens YT1

Agriculture, Journal Year: 2024, Volume and Issue: 14(7), P. 1193 - 1193

Published: July 19, 2024

The purpose of this study is to discover and excavate more key factors signaling pathways that regulate the formation intensity biofilms fully reveal possible models affecting biofilm formation. By using gene homologous recombination bioinformatics technology, a MutL protein-directed deletion mutant strain was successfully constructed. growth status observed, it confirmed that, except for change in cell morphology, there were no significant differences reproduction between wild-type strain. induced technique, decrease verified. plate confrontation test inhibitory ability against rice blast fungus not significantly different from WT colonization on stems tested, lower than In terms prevention control effect disease, showed decrease. transcriptomic big data, pathway expression during analyzed. analysis revealed correlation with previously reported spo0A tapA-sipW-tasA pathways. factor capB polyglutamic acid pathway, which affects biological model, found have expression. A mechanical hypothesis proposed: may participate regulating Bacillus by glutamic acid.

Language: Английский

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Prospection of potential cellulase producing filamentous fungi from corn silage

OBSERVATÓRIO DE LA ECONOMÍA LATINOAMERICANA, Journal Year: 2024, Volume and Issue: 22(11), P. e7939 - e7939

Published: Nov. 26, 2024

The prospection of filamentous fungi is essential for understanding fungal biodiversity and exploring its potential in biotechnological processes. Among applications, cellulase production stands out; it used clarifying juice, reducing food viscosity, producing fermentable sugars from plant residues. This study soughr to isolate corn silage samples collected Gouveia, Minas Gerais, Brazil, using three methods: direct contact, dilution, pour plate. isolation was performed on a solid culture medium containing carboxymethylcellulose at 30 °C, with monitoring the plates seven days. Eleven were isolated, all obtained by plate method. Once spot transfers performed, microorganisms maintained °C macromorphological characterization after eight results showed primarily velvety-textured mycelia smooth surface, regular edges, flat topography, white mycelium color, dry appearance. Regarding enzymatic activity, halos up 27 mm observed some samples, indicating production. Given diversity characteristics, concluded that there plurality isolated cellulolytic enzyme

Language: Английский

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The impact of surface charge on the interaction of cholesterol-free fusogenic liposomes with planktonic microbial cells and biofilms

International Journal of Pharmaceutics, Journal Year: 2024, Volume and Issue: unknown, P. 125088 - 125088

Published: Dec. 1, 2024

Language: Английский

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Mycobiology of Dental Unit Water: A Systematic Scoping Review

International Dental Journal, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 1, 2024

Language: Английский

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