Baicalin Relieves Glaesserella parasuis-Triggered Immunosuppression Through Polarization via MIF/CD74 Signaling Pathway in Piglets

Biomolecules, Journal Year: 2025, Volume and Issue: 15(5), P. 640 - 640

Published: April 29, 2025

Glaesserella parasuis (G. parasuis) infection is responsible for Glässer’s disease in pigs. G. could trigger piglet immunosuppression, but the mechanism of inducing immunosuppression by remains unknown. Macrophage migration inhibitory factor (MIF)/CD74 axis has been shown to participate inflammation response and function MIF/CD74 during elicited not fully explored. This experiment explored efficacy baicalin on alleviation through regulating polarization via signaling pathway. Our data indicated that reduced IL-1β, IL-6, IL-8, IL-18, TNF-α, COX-2 expression, regulated expression spleen. Immunohistochemistry analysis showed enhanced CD74 protein levels spleen piglets induced parasuis. Baicalin PI3K/Akt/mTOR pathway RAF/MEK/ERK activation, modified autophagy-related proteins Beclin-1, P62, LC3B, promoted M2 M1 polarization, CD3, CD4, CD8, TIM3 study reveals important functions parasuis-induced may offer a new therapeutic method control infection.

Language: Английский

Baicalin attenuates PD-1/PD-L1 axis-induced immunosuppression in piglets challenged with Glaesserella parasuis by inhibiting the PI3K/Akt/mTOR and RAS/MEK/ERK signalling pathways

Veterinary Research, Journal Year: 2024, Volume and Issue: 55(1)

Published: July 29, 2024

Abstract Infection of piglets with Glaesserella parasuis ( G. ) induces host immunosuppression. However, the mechanism underlying immunosuppression remains unclear. Activation PD-1/PD-L1 axis has been shown to trigger Baicalin possesses anti-inflammatory and immunomodulatory functions. whether baicalin inhibits activation thus alleviates not investigated. In this study, effect on attenuation piglet induced by was evaluated. Seventy were randomly divided into control group, infection levamisole BMS-1 25 mg/kg 50 group 100 group. Following pretreatment levamisole, or baicalin, challenged 1 × 10 8 CFU . Our results showed that modified routine blood indicators biochemical parameters; downregulated IL-1β, IL-10, IL-18, TNF-α IFN-γ mRNA expression; upregulated IL-2 IL-8 expression in blood. Baicalin, increased proportions CD3 + T cells, CD4 CD8 cells – CD21 B splenocyte population, blood, inhibited TIM-3 activation. reduced p-PI3K, p-Akt, p-mTOR expression, p-MEK1/2/MEK1/2 p-ERK1/2/ERK1/2 ratios RAS expression. provided substantial protection against challenge relieved tissue histopathological damage. findings might provide new strategies for controlling other immunosuppressive diseases.

Language: Английский

LAMTOR1/mTORC1 promotes CD276 to induce immunosuppression via PI3K/Akt/MMP signaling pathway in Clostridium perfringens–induced necrotic enteritis of laying hens

Poultry Science, Journal Year: 2024, Volume and Issue: 103(12), P. 104216 - 104216

Published: Sept. 2, 2024

Clostridium perfringens (C. perfringens) causes avian necrotic enteritis, leading to huge economic losses the poultry industry. This pathogen induces host immunosuppression; however, molecular mechanism is still unclear. Thus, we established a laying hen infection model explore this mechanism. We randomly divided 20 one-old-day hens into control and groups. The group was infected intragastrically with 1 × 10

Language: Английский