NCAPD3-mediated ferroptosis of 2,3,5,4’-Tetrahydroxystilbene-2-O-β-D-glucoside inhibits proliferation in T47D cells

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 29, 2025

Objective Non-SMC condensin II complex subunit D3 (NCAPD3) has recently been demonstrated as a crucial oncogenic factor, nevertheless, the biological role of NCAPD3 in pathogenesis breast cancer not elucidated. Evidence suggests that targeting ferroptosis can inhibit progression cancer. Moreover, 2,3,5,4’-Tetrahydroxystilbene-2-O-β-D-glucoside (THSG) could modulate MCF-7 cell proliferation our previous study. Therefore, we aimed to investigate potential mechanism by which mediates THSG inhibition T47D full-length transcriptome sequencing. Methods Alternative splicing analysis was performed based on sequencing and overlapping genes differentially expressed transcripts (DETs) differential alternative (diAS) were obtained. Further, RT-PCR used validate type splicing. And hub (transcripts) selected using bioinformatics analysis, quantitative polymerase chain reaction (qPCR) Western blotting (WB). cycle assessed flow cytometry WB respectively. Mechanically, viability clone formation detected Biochemical kit. siRNA Ncapd3 transfected into cells detect expression levels ferroptosis-related proteins (WB) (MTT). Results 40 DETs diAS obtained consistent with sequencing, ( Ncapd3-203 ) is key gene (transcript), also highly mRNA protein expression. induce arrest G2/M stage reduce (xCT GPx4). found inhibits cells, GPx4) expression, regulated THSG-suppressing effect cells. Conclusion -dependent ferroptosis, provided novel insights targeted strategy for

Language: Английский

Runx1 activates the transformation of adipocytes into cancer-associated adipocytes by downregulating Plin1

Experimental Cell Research, Journal Year: 2025, Volume and Issue: unknown, P. 114573 - 114573

Published: April 1, 2025

Language: Английский

Targeting lipid metabolism via nanomedicine: a prospective strategy for cancer therapy

Biomaterials, Journal Year: 2024, Volume and Issue: 317, P. 123022 - 123022

Published: Dec. 15, 2024

Language: Английский