Comprehensive multiomics analysis identifies PYCARD as a key pyroptosis-related gene in osteoarthritis synovial macrophages

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: March 24, 2025

Background Osteoarthritis (OA) is a chronic joint disease that significantly impairs quality of life. Synovitis plays pivotal role in OA progression, and pyroptosis, form programmed cell death associated with innate immune inflammation, may contribute to the pathogenesis synovitis. Nevertheless, precise pyroptosis remains poorly understood. Methods We performed an analysis bulk RNA sequencing data examine expression profiles pyroptosis-related genes synovium. A LASSO-Cox regression model was employed identify genes. Single-cell were used validate these specific synovial clusters. Differentially expressed (DEGs) macrophages high or low levels core subjected enrichment analysis. protein-protein interaction (PPI) network constructed hub genes, potential therapeutic compounds predicted. Consensus clustering correlations between status. After identifying PYCARD as gene macrophages, we assessed synovium validated its related M1 macrophages. Results total twenty DEGs identified, six selected through LASSO regression. identified key Furthermore, 57 targeting Validation confirmed upregulation Conclusion identified. This study offers valuable insights into treatment targets for OA.

Language: Английский

Advancing osteoarthritis research: the role of AI in clinical, imaging and omics fields

Bone Research, Journal Year: 2025, Volume and Issue: 13(1)

Published: April 22, 2025

Language: Английский

From cells to clinic: Single-cell transcriptomics shaping the future of orthopedics

Journal of Orthopaedic Translation, Journal Year: 2025, Volume and Issue: 53, P. 1 - 11

Published: May 28, 2025

Language: Английский