Antimicrobial Nanoparticles Composed of Zein and Arginine-Phenylalanine-Based Surfactants for Wound Related Infections: Antioxidant and Skin-Related Anti-Enzymatic Activities and Toxicity DOI Creative Commons
Francisco Fábio Oliveira de Sousa, Zakaria Hafidi, M. Teresa García

et al.

Antibiotics, Journal Year: 2024, Volume and Issue: 13(12), P. 1149 - 1149

Published: Dec. 1, 2024

Background/Objectives: Cationic surfactants are potential antimicrobial candidates. Even so, they the foremost irritative and incompatible group, which limits their usage. The incorporation of in biopolymer-based nanoparticles is a feasible strategy to improve efficacy reduce those drawbacks. Methods: Surfactants with one amino acid on polar head (lauroyl arginine methyl ester—LAM phenylalanine dodecyl amide—PNHC12) two acids heads, arginine-phenylalanine (Lauroyl esther—C12PAM phenylalanine-arginine amide—PANHC12) were loaded zein nanoparticles. Their antibiofilm activities evaluated. Also, inhibitory over skin-related enzymes accessed silico vitro, while cytotoxicity was determined comparatively immortal human keratinocytes (HaCaT) fibroblasts (3T3). Finally, Vibrio fisheri luminescence reduction test used detect its ecotoxicity. Results: obtained successfully exhibited good biocide activity against wide range pathogenic bacteria yeasts. found active assayed: elastase > tyrosinase collagenase lipoxygenase, superior when nanoencapsulated lipoxygenase. corresponding presented acceptable cytotoxic levels, except for PNHC12 both forms, ecotoxicity limited acceptable. Conclusions: Accordingly, nanoencapsulation be smart enhance selectivity representative skin connective tissues cell lines. These biological properties render surfactant as promising candidates tissue repairing applications wound treatments.

Language: Английский

Crosstalk Between Antioxidants and Adipogenesis: Mechanistic Pathways and Their Roles in Metabolic Health DOI Creative Commons
M.W. Fu,

Kyung‐Sik Yoon,

Joohun Ha

et al.

Antioxidants, Journal Year: 2025, Volume and Issue: 14(2), P. 203 - 203

Published: Feb. 10, 2025

The interplay between oxidative stress and adipogenesis is a critical factor in the development of obesity its associated metabolic disorders. Excessive reactive oxygen species (ROS) disrupt key transcription factors such as peroxisome proliferator-activated receptor gamma (PPARγ) CCAAT/enhancer-binding protein alpha (C/EBPα), impairing lipid metabolism, promoting adipocyte dysfunction, exacerbating inflammation insulin resistance. Antioxidants, classified endogenous (e.g., glutathione, superoxide dismutase, catalase) exogenous polyphenols, flavonoids, vitamins C E), are pivotal mitigating these effects by restoring redox balance preserving functionality. Endogenous antioxidants neutralize ROS safeguard cellular structures; however, under heightened stress, defenses often insufficient, necessitating dietary supplementation. Exogenous derived from plant-based sources, polyphenols vitamins, act through direct scavenging, upregulation antioxidant enzymes, modulation signaling pathways like nuclear kappa B (NF-κB) PPARγ, reducing peroxidation, inflammation, dysfunction. Furthermore, they influence epigenetic regulation transcriptional networks to restore differentiation limit accumulation. Antioxidant-rich diets, including Mediterranean diet, strongly with improved health, reduced rates, enhanced sensitivity. Advances personalized therapies, guided biomarkers supported novel delivery systems, present promising avenues for optimizing therapeutic interventions. This review, "Crosstalk Between Antioxidants Adipogenesis: Mechanistic Pathways Their Role Metabolic Health", highlights mechanistic which regulate enhance health.

Language: Английский

Citations

1
OPTIMIZATION OF THE CONDITION TO PRODUCE ZEIN-ACALYPHA INDICA. l LEAF EXTRACT NANOPARTICLES AS ANTIOXIDANTS DOI Open Access
Dini Hanifa,

Fitratul Ilahi,

Yefrida Yefrida

et al.

International Journal of Applied Pharmaceutics, Journal Year: 2025, Volume and Issue: unknown, P. 43 - 50

Published: Feb. 24, 2025

Objective: Acalypha indica L. (A. L.) is an herbal plant predominantly found in the wet tropics and known for its diverse activities. One way to increase biological activities by making it as nanoparticle form. This study aimed optimize condition produce zein-leaf A. Nanoparticles (NPZA) antioxidant. Methods: The nanoparticles optimization was developed using a central composite design, employing two solvents extraction: 50% ethanol (NPZAE50%) methanol p. (NPZAM). optimized conditions include amount of extract, zein, sonication time. antioxidant capacity evaluated Diphenyl Picryl Hydrazyl Method, while were characterized UV/Vis Spectrophotometry, Fourier Transform Infrared Spectroscopy (FTIR), Particle Size Analyzer (PSA), Transmission Electron Microscope (TEM). Results: optimum on 100 mg extract 50%, 150 time 20 min, with 0.935±0.15 mmol AAE/ml NPZAE50%. average particle size NPZAE50% 27.5 nm spherical morphology. Conclusion: In conclusion, highest achieved 117 175 23 min sonication, formula showing best results NPZAM.

Language: Английский

Citations

0
Nanoparticles based on whey and soy proteins enhance the antioxidant activity of phenolic compound extract from Cantaloupe melon pulp flour (Cucumis melo L.) DOI
Tatiana Quintanilha Soares da Silva,

Yasmim Pessoa de Oliveira,

Leticya Bianca Almeida de Carvalho

et al.

Food Chemistry, Journal Year: 2024, Volume and Issue: unknown, P. 141738 - 141738

Published: Oct. 1, 2024

Language: Английский

Citations

1
Antimicrobial Nanoparticles Composed of Zein and Arginine-Phenylalanine-Based Surfactants for Wound Related Infections: Antioxidant and Skin-Related Anti-Enzymatic Activities and Toxicity DOI Creative Commons
Francisco Fábio Oliveira de Sousa, Zakaria Hafidi, M. Teresa García

et al.

Antibiotics, Journal Year: 2024, Volume and Issue: 13(12), P. 1149 - 1149

Published: Dec. 1, 2024

Background/Objectives: Cationic surfactants are potential antimicrobial candidates. Even so, they the foremost irritative and incompatible group, which limits their usage. The incorporation of in biopolymer-based nanoparticles is a feasible strategy to improve efficacy reduce those drawbacks. Methods: Surfactants with one amino acid on polar head (lauroyl arginine methyl ester—LAM phenylalanine dodecyl amide—PNHC12) two acids heads, arginine-phenylalanine (Lauroyl esther—C12PAM phenylalanine-arginine amide—PANHC12) were loaded zein nanoparticles. Their antibiofilm activities evaluated. Also, inhibitory over skin-related enzymes accessed silico vitro, while cytotoxicity was determined comparatively immortal human keratinocytes (HaCaT) fibroblasts (3T3). Finally, Vibrio fisheri luminescence reduction test used detect its ecotoxicity. Results: obtained successfully exhibited good biocide activity against wide range pathogenic bacteria yeasts. found active assayed: elastase > tyrosinase collagenase lipoxygenase, superior when nanoencapsulated lipoxygenase. corresponding presented acceptable cytotoxic levels, except for PNHC12 both forms, ecotoxicity limited acceptable. Conclusions: Accordingly, nanoencapsulation be smart enhance selectivity representative skin connective tissues cell lines. These biological properties render surfactant as promising candidates tissue repairing applications wound treatments.

Language: Английский

Citations

0