Cuproptosis-related gene ACAD8 inhibits the metastatic ability of colorectal cancer by inducing cuproptosis

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: April 3, 2025

Background Distant metastasis of colorectal cancer (CRC) significantly impacts patient prognosis. Cuproptosis is a new form copper ion-dependent cell death. However, whether cuproptosis-related genes (CRGs) play role in the metastatic potential CRC remains unclear. This study focuses on CRGs-ACAD8 to explore its and mechanism (mCRC). Methods Clinical sample data from TCGA, GEO, Fujian Provincial Hospital patients were integrated analyze ACAD8 expression association with diagnosis prognosis CRC. Small interfering RNA, immunohistochemistry, colony formation, wound-healing assays so used evaluate biological functions ACAD8. Bioinformatics was applied investigate relationships immune infiltration, chemotherapy sensitivity, signaling pathways. Results reduced mCRC demonstrated excellent diagnostic performance. Patients high had better survival. closely associated enhanced sensitivity. Pathway enrichment analysis suggested that might inhibit by regulating pathways such as response metal ions tight junction organization. Finally, experiments confirmed positive correlation between levels mRNA expression, CuCl 2 upregulating expression. Knockdown induced cuproptosis. inhibited proliferation, stemness, migratory abilities cells, while si attenuated these effects. Moreover, sensitivity cells oxaliplatin 5-fluorouracil, whereas diminished this chemosensitizing effect. Conclusion As novel tumor suppressor, low holds prognostic value may contribute precise treatment infiltration

Language: Английский

The Role of Proteomics and Genomics in the Development of Colorectal Cancer Diagnostic Tools and Potential New Treatments

ACS Pharmacology & Translational Science, Journal Year: 2025, Volume and Issue: unknown

Published: April 10, 2025

Language: Английский