VGluT3 BNST neurons transmit GABA and restrict feeding without affecting rewarding or aversive processing

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 2, 2025

The bed nucleus of the stria terminalis (BNST) is involved in feeding, reward, aversion, and anxiety-like behavior. We identify BNST neurons defined by expression vesicular glutamate transporter 3, VGluT3. VGluT3 were localized to anteromedial BNST, molecularly distinct from accumbal neurons, co-express GABA (VGaT). Cell-type specific presynaptic processes identified arcuate (ARC) paraventricular hypothalamus (PVN), regions critical for feeding homeostatic regulation. Whole-cell patch-clamp electrophysiology revealed that, while these VGaT, they functionally transmit both ARC PVN, with rare co-transmission ARC. Neuronal recordings showed greater calcium-dependent signaling response sucrose consumption sated compared fasted. When fasted, optogenetic stimulation decreased using several conditions but not when occurred prior access, suggesting that activation concurrent fasted state reduces feeding. during paradigms (novelty-suppressed open field, elevated zero maze) real-time place conditioning resulted no changes or reward/aversion interpret data such represent a unique cellular population within provides inhibitory input hypothalamic decrease without affecting

Language: Английский

GABAB receptor-mediated potentiation of ventral medial habenula glutamatergic transmission in GABAergic and glutamatergic interpeduncular nucleus neurons

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 3, 2025

Abstract The medial habenula (MHb)-interpeduncular nucleus (IPN) pathway plays an important role in information transferring between the forebrain and midbrain. MHb-IPN has been implicated regulation of fear behavior nicotine addiction. synapses ventral MHb IPN show a unique property, i.e., enhancement synaptic transmission upon activation GABA B receptors. This receptor-mediated potentiation regulating memory. Although is known to contain parvalbumin (PV) somatostatin (SST) GABAergic neurons vesicular glutamate transporter 3 (VGLUT3)-expressing neurons, it unknown how receptor affects MHb-mediated glutamatergic onto these three subtypes neurons. Our studies robust connectivity from PV SST IPN, while VGLUT3 weak. receptors produces we observed modest effect Despite diminished basal causes transient conversion non-responding into active some Thus, our results strong compared VGLUT3-expressing Furthermore, differential on PV, SST, IPN.

Language: Английский

Deciphering the Functions of Raphe–Hippocampal Serotonergic and Glutamatergic Circuits and their Deficits in Alzheimer’s Disease

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(3), P. 1234 - 1234

Published: Jan. 30, 2025

Subcortical innervation of the hippocampus by raphe nucleus is essential for emotional and cognitive control. The two major afferents from to originate serotonergic glutamatergic neurons, which control hippocampal inhibitory network, theta activity, synaptic plasticity have been extensively explored in growing body literature, whereas those circuits received little attention. Notably, both between are disrupted Alzheimer's disease (AD), may contribute initiation progression behavioral psychological symptoms dementia. Thus, deciphering mechanism underlying abnormal raphe-hippocampal AD crucial prevent dementia-associated symptoms. In this review, we summarize anatomical, neurochemical, electrophysiological diversity nuclei as well architecture circuitry. We then elucidate subcortical activity their role regulation emotion cognition. Additionally, present an overview pathogenesis analyze available therapies that can potentially be used clinically alleviate neuropsychiatric decline course.

Language: Английский