State of the art CRISPR-based strategies for cancer diagnostics and treatment DOI Creative Commons
Emma Di Carlo, Carlo Sorrentino

Biomarker Research, Journal Year: 2024, Volume and Issue: 12(1)

Published: Dec. 18, 2024

Abstract Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) technology is a groundbreaking and dynamic molecular tool for DNA RNA “surgery”. CRISPR/Cas9 the most widely applied system in oncology research. It major advancement genome manipulation due to its precision, efficiency, scalability versatility compared previous gene editing methods. has shown great potential not only targeting of oncogenes or genes coding immune checkpoint molecules, engineering T cells, but also epigenomic disturbances, which contribute cancer development progression. proven useful detecting genetic mutations, enabling large-scale screening involved tumor onset, progression drug resistance, speeding up highly targeted therapies tailored immunological profiles patient’s tumor. Furthermore, recently discovered Cas12 Cas13 systems have expanded Cas9-based applications, providing new opportunities diagnosis treatment cancer. In addition traditional cis-cleavage, they exhibit trans-cleavage activity, enables their use as sensitive specific diagnostic tools. Diagnostic platforms like DETECTR, employs enzyme, that cuts single-stranded reporters, SHERLOCK, uses Cas12, Cas13, specifically target cleave RNA, can be exploited speed advance oncological diagnostics. Overall, CRISPR platform improve diagnostics functionality safety engineered cellular medicines. Here, we will emphasize potentially transformative impact field treatments, prognostic approaches, highlight challenges raised by using newly introduced CRISPR-based therapy.

Language: Английский

CRISPR-Cas Systems: A Functional Perspective and Innovations DOI Open Access
Carla Navarro, María P. Díaz, Pablo Durán

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(8), P. 3645 - 3645

Published: April 12, 2025

Adaptation is a fundamental tenet of evolutionary biology and essential for the survival all organisms, including prokaryotes. The evolution clustered regularity exemplifies this principle interspaced short palindromic repeats (CRISPR) associated proteins (Cas), an adaptive immune system that confers resistance to viral infections. By integrating segments genomes into their own, bacteria archaea develop molecular memory enables them mount rapid targeted response upon subsequent challenges. fortuitous discovery mechanism prompted many studies introduced researchers novel tools could potentially be developed from CRISPR-Cas become clinically relevant as biotechnology rapidly advances in area. Thus, deeper understanding underpinnings its possible therapeutic applications required. This review analyses action systems detail summarises developing biotechnological based on CRISPR, opening field further research.

Language: Английский

Citations

0
State of the art CRISPR-based strategies for cancer diagnostics and treatment DOI Creative Commons
Emma Di Carlo, Carlo Sorrentino

Biomarker Research, Journal Year: 2024, Volume and Issue: 12(1)

Published: Dec. 18, 2024

Abstract Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) technology is a groundbreaking and dynamic molecular tool for DNA RNA “surgery”. CRISPR/Cas9 the most widely applied system in oncology research. It major advancement genome manipulation due to its precision, efficiency, scalability versatility compared previous gene editing methods. has shown great potential not only targeting of oncogenes or genes coding immune checkpoint molecules, engineering T cells, but also epigenomic disturbances, which contribute cancer development progression. proven useful detecting genetic mutations, enabling large-scale screening involved tumor onset, progression drug resistance, speeding up highly targeted therapies tailored immunological profiles patient’s tumor. Furthermore, recently discovered Cas12 Cas13 systems have expanded Cas9-based applications, providing new opportunities diagnosis treatment cancer. In addition traditional cis-cleavage, they exhibit trans-cleavage activity, enables their use as sensitive specific diagnostic tools. Diagnostic platforms like DETECTR, employs enzyme, that cuts single-stranded reporters, SHERLOCK, uses Cas12, Cas13, specifically target cleave RNA, can be exploited speed advance oncological diagnostics. Overall, CRISPR platform improve diagnostics functionality safety engineered cellular medicines. Here, we will emphasize potentially transformative impact field treatments, prognostic approaches, highlight challenges raised by using newly introduced CRISPR-based therapy.

Language: Английский

Citations

1