Annexin A3 Represses Endothelial Permeability and Inflammation During Sepsis via Actin Cytoskeleton Modulation DOI Creative Commons
Manyu Xing, Shuang Liang,

Wei Cao

et al.

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: March 28, 2025

Abstract Increased endothelial permeability and a dysregulated inflammatory response play key roles in organ damage sepsis. The role of annexin A3 (ANXA3) regulating inflammation during sepsis is explored using ANXA3 knockout mice primary human umbilical vein cells (HUVECs). absence exacerbated outcomes, including increased mortality, lung injury, leukocyte infiltration, vascular permeability. highly expressed its loss results the formation cytoskeletal stress fibers decrease expression junction proteins zonula occludens (Zo)‐1, (VE)‐cadherin, claudin 5, leading to increase knockdown also upregulates E‐selectin (CD62E) through phosphorylation activating transcription factor 2 (ATF2), which increases monocyte adhesion HUVECs after LPS stimulation. Inhibiting actin polymerization reverse these effects. Thus, stabilizes cytoskeleton, playing protective dysfunction

Language: Английский

Regulating white blood cell activity through the novel Universal Receptive System DOI Open Access
Victor Tetz,

Kristina Kardava,

Maria Vecherkovskaya

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 20, 2025

Abstract The understanding of the mechanisms that control key features immune cells in various disease contexts remains limited, and few techniques are available for manipulating cells. Thus, discovering novel strategies regulating is essential gaining insight into their roles health disease. In this study, we investigated potential recently described Universal Receptive System to regulate human cell functions. This was achieved first time by specifically targeting newly discovered surface-bound DNA RNA-based receptors on leukocytes generating “Leukocyte-Tells.” approach upregulated numerous genes related signaling, migration, endocytosis, phagocytosis pathways. antimicrobial anticancer activities Leukocyte-Tells exceeded activity vitro . some settings, such as antibiofilm experiments, showed up 1,000,000-fold higher than leukocytes. Our findings reveal, time, can orchestrate fundamental properties cells, including enhanced anti-tumor activities. offers a new avenue biology regulation white blood

Language: Английский

Citations

0
Annexin A3 Represses Endothelial Permeability and Inflammation During Sepsis via Actin Cytoskeleton Modulation DOI Creative Commons
Manyu Xing, Shuang Liang,

Wei Cao

et al.

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: March 28, 2025

Abstract Increased endothelial permeability and a dysregulated inflammatory response play key roles in organ damage sepsis. The role of annexin A3 (ANXA3) regulating inflammation during sepsis is explored using ANXA3 knockout mice primary human umbilical vein cells (HUVECs). absence exacerbated outcomes, including increased mortality, lung injury, leukocyte infiltration, vascular permeability. highly expressed its loss results the formation cytoskeletal stress fibers decrease expression junction proteins zonula occludens (Zo)‐1, (VE)‐cadherin, claudin 5, leading to increase knockdown also upregulates E‐selectin (CD62E) through phosphorylation activating transcription factor 2 (ATF2), which increases monocyte adhesion HUVECs after LPS stimulation. Inhibiting actin polymerization reverse these effects. Thus, stabilizes cytoskeleton, playing protective dysfunction

Language: Английский

Citations

0