SIRT5 Alleviates Apoptosis of Vascular Endothelial Cells Under Simulated Microgravity via Desuccinylation of ERO1A DOI Open Access
Yikai Pan, Qian Zhang, Chengfei Li

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(7), P. 2908 - 2908

Published: March 23, 2025

The adverse effects of weightlessness on the human cardiovascular system greatly hinder process long-term and long-distance space exploration. Succinylation is an important type protein post-translational modification. However, whether succinylation modification able to play a role in altered vascular endothelial cell function under microgravity or simulated has not been reported. This study aims investigate quantitative global proteome changes lysine related proteins, seeking facilitate better understanding deconditioning microgravity. LC-MS/MS combined with bioinformatics analysis were used quantitatively detect perspectives at level. Immunoprecipitation Western blot conducted further verify alterations proteins succinylation. A total 132 differentially expressed 164 sites identified umbilical vein cells (HUVECs). Bioinformatics indicates that may potential energy metabolism. In addition, desuccinylase SIRT5 was downregulated regulated levels HUVECs Notably, overexpression effectively protected from apoptosis induced by And Lys396 ERO1A significantly increased Mechanistically, knockdown found induce through ERO1A. These results can provide new ideas for elucidating molecular mechanism dysfunction environments, key targets scientific protective measures against space.

Language: Английский

SIRT5 Alleviates Apoptosis of Vascular Endothelial Cells Under Simulated Microgravity via Desuccinylation of ERO1A DOI Open Access
Yikai Pan, Qian Zhang, Chengfei Li

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(7), P. 2908 - 2908

Published: March 23, 2025

The adverse effects of weightlessness on the human cardiovascular system greatly hinder process long-term and long-distance space exploration. Succinylation is an important type protein post-translational modification. However, whether succinylation modification able to play a role in altered vascular endothelial cell function under microgravity or simulated has not been reported. This study aims investigate quantitative global proteome changes lysine related proteins, seeking facilitate better understanding deconditioning microgravity. LC-MS/MS combined with bioinformatics analysis were used quantitatively detect perspectives at level. Immunoprecipitation Western blot conducted further verify alterations proteins succinylation. A total 132 differentially expressed 164 sites identified umbilical vein cells (HUVECs). Bioinformatics indicates that may potential energy metabolism. In addition, desuccinylase SIRT5 was downregulated regulated levels HUVECs Notably, overexpression effectively protected from apoptosis induced by And Lys396 ERO1A significantly increased Mechanistically, knockdown found induce through ERO1A. These results can provide new ideas for elucidating molecular mechanism dysfunction environments, key targets scientific protective measures against space.

Language: Английский

Citations

0